Restriction map for
pFN21AE5352
(Linear) (Six-base) MAPSORT of: KIEE5352.seq Check: 5447 from: 1 to: 945
KIEE5352.orf
With 182 enzymes: SgfI *
December 5, 2014 13:01 ..
Acc65I G'GTAC_C
Cuts at: 0 21 945
Size: 21 924
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 522 737 945
Size: 290 232 215 208
Fragments arranged by size:
290 232 215 208
AhdI GACnn_n'nnGTC
Cuts at: 0 901 945
Size: 901 44
ApoI r'AATT_y
Cuts at: 0 286 406 830 945
Size: 286 120 424 115
Fragments arranged by size:
424 286 120 115
AvaI C'yCGr_G
Cuts at: 0 44 545 945
Size: 44 501 400
Fragments arranged by size:
501 400 44
BanI G'GyrC_C
Cuts at: 0 21 945
Size: 21 924
BbsI GAAGACnn'nnnn_
Cuts at: 0 341 945
Size: 341 604
BmrI ACTGGGnnnn_n'
Cuts at: 0 549 945
Size: 549 396
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 101 945
Size: 101 844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 945
Size: 52 893
Bpu10I CC'TnA_GC
Cuts at: 0 145 945
Size: 145 800
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 719 749 945
Size: 719 30 196
Fragments arranged by size:
719 196 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 945
Size: 809 136
BsmI GAATG_Cn'
Cuts at: 0 772 945
Size: 772 173
BsrGI T'GTAC_A
Cuts at: 0 118 945
Size: 118 827
BstAPI GCAn_nnn'nTGC
Cuts at: 0 57 945
Size: 57 888
BstBI TT'CG_AA
Cuts at: 0 444 945
Size: 444 501
BstYI r'GATC_y
Cuts at: 0 801 945
Size: 801 144
DraI TTT'AAA
Cuts at: 0 569 945
Size: 569 376
DrdI GACnn_nn'nnGTC
Cuts at: 0 253 945
Size: 253 692
EaeI y'GGCC_r
Cuts at: 0 2 857 945
Size: 2 855 88
Fragments arranged by size:
855 88 2
EarI CTCTTCn'nnn_
Cuts at: 0 266 541 713 945
Size: 266 275 172 232
Fragments arranged by size:
275 266 232 172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 290 522 737 945
Size: 52 238 232 215 208
Fragments arranged by size:
238 232 215 208 52
EcoRI G'AATT_C
Cuts at: 0 286 406 945
Size: 286 120 539
Fragments arranged by size:
539 286 120
EcoRV GAT'ATC
Cuts at: 0 360 945
Size: 360 585
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 725 757 887 919 945
Size: 725 32 130 32 26
Fragments arranged by size:
725 130 32 32 26
KpnI G_GTAC'C
Cuts at: 0 25 945
Size: 25 920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 135 945
Size: 74 61 810
Fragments arranged by size:
810 74 61
MslI CAynn'nnrTG
Cuts at: 0 482 765 945
Size: 482 283 180
Fragments arranged by size:
482 283 180
MspA1I CmG'CkG
Cuts at: 0 268 945
Size: 268 677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 945
Size: 409 32 504
Fragments arranged by size:
504 409 32
PstI C_TGCA'G
Cuts at: 0 192 945
Size: 192 753
PvuII CAG'CTG
Cuts at: 0 268 945
Size: 268 677
ScaI AGT'ACT
Cuts at: 0 590 945
Size: 590 355
SfcI C'TryA_G
Cuts at: 0 188 806 927 945
Size: 188 618 121 18
Fragments arranged by size:
618 188 121 18
SmlI C'TyrA_G
Cuts at: 0 545 945
Size: 545 400
StyI C'CwwG_G
Cuts at: 0 635 945
Size: 635 310
TatI w'GTAC_w
Cuts at: 0 118 531 588 945
Size: 118 413 57 357
Fragments arranged by size:
413 357 118 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 719 751 945
Size: 719 32 194
Fragments arranged by size:
719 194 32
XhoI C'TCGA_G
Cuts at: 0 545 945
Size: 545 400
XmnI GAAnn'nnTTC
Cuts at: 0 450 945
Size: 450 495
Enzymes that do cut:
Acc65I AcuI AhdI ApoI AvaI BanI BbsI BmrI
BplI BpmI Bpu10I BsaXI BsgI BsmI BsrGI BstAPI
BstBI BstYI DraI DrdI EaeI EarI Eco57MI EcoRI
EcoRV Hin4I KpnI MmeI MslI MspA1I PpiI PstI
PvuII ScaI SfcI SmlI StyI TatI TstI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII
EagI EciI EcoICRI EcoNI EcoO109I FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XcmI XmaI ZraI