(Linear) (Six-base) MAPSORT of: KIEE5351.seq  Check: 1539  from: 1  to: 918



KIEE5351.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:56  ..

AclI AA'CG_TT

Cuts at:      0     374     918 
   Size:        374     544
 
AfeI AGC'GCT

Cuts at:      0     511     918 
   Size:        511     407
 
AleI CACnn'nnGTG

Cuts at:      0     679     918 
   Size:        679     239
 
ApoI r'AATT_y

Cuts at:      0     239     598     692     918 
   Size:        239     359      94     226

  Fragments arranged by size: 

                 359     239     226      94
 
AseI AT'TA_AT

Cuts at:      0     866     918 
   Size:        866      52
 
BbsI GAAGACnn'nnnn_

Cuts at:      0       2     918 
   Size:          2     916
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     421     918 
   Size:        421     497
 
BsaWI w'CCGG_w

Cuts at:      0      51     837     918 
   Size:         51     786      81

  Fragments arranged by size: 

                 786      81      51
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      75     918 
   Size:         75     843
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     828     918 
   Size:        828      90
 
BsmI GAATG_Cn'

Cuts at:      0     292     918 
   Size:        292     626
 
BsrGI T'GTAC_A

Cuts at:      0     829     918 
   Size:        829      89
 
EcoRI G'AATT_C

Cuts at:      0     692     918 
   Size:        692     226
 
EcoRV GAT'ATC

Cuts at:      0     147     918 
   Size:        147     771
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     104     214     918 
   Size:        104     110     704

  Fragments arranged by size: 

                 704     110     104
 
HaeII r_GCGC'y

Cuts at:      0     513     918 
   Size:        513     405
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342     404     436     771     803     888     918 
   Size:        310      32      62      32     335      32      85      30

  Fragments arranged by size: 

                 335     310      85      62      32      32      32      30
            
 
MfeI C'AATT_G

Cuts at:      0     591     918 
   Size:        591     327
 
MslI CAynn'nnrTG

Cuts at:      0     285     679     918 
   Size:        285     394     239

  Fragments arranged by size: 

                 394     285     239
 
MspA1I CmG'CkG

Cuts at:      0     142     918 
   Size:        142     776
 
NdeI CA'TA_TG

Cuts at:      0     282     918 
   Size:        282     636
 
NspI r_CATG'y

Cuts at:      0     886     918 
   Size:        886      32
 
PstI C_TGCA'G

Cuts at:      0     449     918 
   Size:        449     469
 
PvuII CAG'CTG

Cuts at:      0     142     918 
   Size:        142     776
 
ScaI AGT'ACT

Cuts at:      0     520     701     918 
   Size:        520     181     217

  Fragments arranged by size: 

                 520     217     181
 
SfcI C'TryA_G

Cuts at:      0     445     918 
   Size:        445     473
 
SspI AAT'ATT

Cuts at:      0     114     271     918 
   Size:        114     157     647

  Fragments arranged by size: 

                 647     157     114
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     641     918 
   Size:        641     277
 
TatI w'GTAC_w

Cuts at:      0     518     652     699     829     918 
   Size:        518     134      47     130      89

  Fragments arranged by size: 

                 518     134     130      89      47
 
XmnI GAAnn'nnTTC

Cuts at:      0       9     918 
   Size:          9     909
 

 Enzymes that do cut:

     AclI     AfeI     AleI     ApoI     AseI     BbsI     BmrI    BsaWI
    BseRI     BsgI     BsmI    BsrGI    EcoRI    EcoRV     FalI    HaeII
    Hin4I     MfeI     MslI   MspA1I     NdeI     NspI     PstI    PvuII
     ScaI     SfcI     SspI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI