(Linear) (Six-base) MAPSORT of: KIEE5349.seq  Check: 3923  from: 1  to: 891



KIEE5349.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     866     891 
   Size:        866      25
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     437     891 
   Size:        437     454
 
ApoI r'AATT_y

Cuts at:      0     838     891 
   Size:        838      53
 
AvaI C'yCGr_G

Cuts at:      0     648     891 
   Size:        648     243
 
BanI G'GyrC_C

Cuts at:      0     463     891 
   Size:        463     428
 
BanII G_rGCy'C

Cuts at:      0      31     891 
   Size:         31     860
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     410     891 
   Size:        410     481
 
BglI GCCn_nnn'nGGC

Cuts at:      0     117     430     891 
   Size:        117     313     461

  Fragments arranged by size: 

                 461     313     117
 
BglII A'GATC_T

Cuts at:      0     337     681     891 
   Size:        337     344     210

  Fragments arranged by size: 

                 344     337     210
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646     891 
   Size:        646     245
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     891 
   Size:         19     872
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     762     891 
   Size:        762     129
 
BsiHKAI G_wGCw'C

Cuts at:      0     543     891 
   Size:        543     348
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     543     891 
   Size:         31     512     348

  Fragments arranged by size: 

                 512     348      31
 
BspHI T'CATG_A

Cuts at:      0      69     132     657     891 
   Size:         69      63     525     234

  Fragments arranged by size: 

                 525     234      69      63
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     366     784     891 
   Size:        366     418     107

  Fragments arranged by size: 

                 418     366     107
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     298     463     891 
   Size:        298     165     428

  Fragments arranged by size: 

                 428     298     165
 
BstYI r'GATC_y

Cuts at:      0     337     681     891 
   Size:        337     344     210

  Fragments arranged by size: 

                 344     337     210
 
BtsI GCAGTG_nn'

Cuts at:      0     749     818     891 
   Size:        749      69      73

  Fragments arranged by size: 

                 749      73      69
 
DraI TTT'AAA

Cuts at:      0     450     891 
   Size:        450     441
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646     866     891 
   Size:        646     220      25

  Fragments arranged by size: 

                 646     220      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     155     891 
   Size:        155     736
 
FspI TGC'GCA

Cuts at:      0     577     891 
   Size:        577     314
 
HaeII r_GCGC'y

Cuts at:      0      41     891 
   Size:         41     850
 
HindIII A'AGCT_T

Cuts at:      0     828     891 
   Size:        828      63
 
MfeI C'AATT_G

Cuts at:      0     323     891 
   Size:        323     568
 
MslI CAynn'nnrTG

Cuts at:      0     791     891 
   Size:        791     100
 
MspA1I CmG'CkG

Cuts at:      0     624     891 
   Size:        624     267
 
NdeI CA'TA_TG

Cuts at:      0     545     891 
   Size:        545     346
 
NspI r_CATG'y

Cuts at:      0     296     561     891 
   Size:        296     265     330

  Fragments arranged by size: 

                 330     296     265
 
PsiI TTA'TAA

Cuts at:      0     164     891 
   Size:        164     727
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      50      82     845     877     891 
   Size:         50      32     763      32      14

  Fragments arranged by size: 

                 763      50      32      32      14
 
PvuII CAG'CTG

Cuts at:      0     624     891 
   Size:        624     267
 
SfcI C'TryA_G

Cuts at:      0     155     891 
   Size:        155     736
 
SmlI C'TyrA_G

Cuts at:      0     648     891 
   Size:        648     243
 
SspI AAT'ATT

Cuts at:      0     720     891 
   Size:        720     171
 
StyI C'CwwG_G

Cuts at:      0     865     891 
   Size:        865      26
 
XbaI T'CTAG_A

Cuts at:      0     762     891 
   Size:        762     129
 
XhoI C'TCGA_G

Cuts at:      0     648     891 
   Size:        648     243
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     AvaI     BanI    BanII     BbsI     BglI
    BglII     BpmI    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
   BstAPI    BstYI     BtsI     DraI  Eco57MI    EcoNI     FspI    HaeII
  HindIII     MfeI     MslI   MspA1I     NdeI     NspI     PsiI     PsrI
    PvuII     SfcI     SmlI     SspI     StyI     XbaI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI    FspAI    Hin4I   HincII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI     XmnI     ZraI