Restriction map for
pFN21AE5348
(Linear) (Six-base) MAPSORT of: KIEE5348.seq Check: 9227 from: 1 to: 855
KIEE5348.orf
With 182 enzymes: SgfI *
January 29, 2014 11:56 ..
AclI AA'CG_TT
Cuts at: 0 659 855
Size: 659 196
AfeI AGC'GCT
Cuts at: 0 145 504 855
Size: 145 359 351
Fragments arranged by size:
359 351 145
AhdI GACnn_n'nnGTC
Cuts at: 0 296 855
Size: 296 559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 855
Size: 208 647
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 260 292 855
Size: 260 32 563
Fragments arranged by size:
563 260 32
AlwNI CAG_nnn'CTG
Cuts at: 0 457 855
Size: 457 398
AvaI C'yCGr_G
Cuts at: 0 409 855
Size: 409 446
BanII G_rGCy'C
Cuts at: 0 750 855
Size: 750 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 824 855
Size: 824 31
BciVI GTATCCnnnnn_n'
Cuts at: 0 95 855
Size: 95 760
BglII A'GATC_T
Cuts at: 0 615 855
Size: 615 240
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 855
Size: 186 669
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 509 855
Size: 509 346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 287 855
Size: 168 119 568
Fragments arranged by size:
568 168 119
Bpu10I CC'TnA_GC
Cuts at: 0 419 855
Size: 419 436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 855
Size: 223 632
BsaI GGTCTCn'nnnn_
Cuts at: 0 201 855
Size: 201 654
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 141 171 260 290 855
Size: 141 30 89 30 565
Fragments arranged by size:
565 141 89 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 855
Size: 130 725
BsiHKAI G_wGCw'C
Cuts at: 0 51 694 750 855
Size: 51 643 56 105
Fragments arranged by size:
643 105 56 51
BsmI GAATG_Cn'
Cuts at: 0 395 855
Size: 395 460
Bsp1286I G_dGCh'C
Cuts at: 0 51 694 750 855
Size: 51 643 56 105
Fragments arranged by size:
643 105 56 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 840 855
Size: 840 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 457 855
Size: 457 398
BstYI r'GATC_y
Cuts at: 0 615 855
Size: 615 240
DraI TTT'AAA
Cuts at: 0 261 383 549 855
Size: 261 122 166 306
Fragments arranged by size:
306 261 166 122
EcoICRI GAG'CTC
Cuts at: 0 748 855
Size: 748 107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 287 855
Size: 168 119 568
Fragments arranged by size:
568 168 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 690 855
Size: 690 165
HaeII r_GCGC'y
Cuts at: 0 147 506 855
Size: 147 359 349
Fragments arranged by size:
359 349 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 33 65 282 314 855
Size: 33 32 217 32 541
Fragments arranged by size:
541 217 33 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 855
Size: 268 587
MslI CAynn'nnrTG
Cuts at: 0 158 855
Size: 158 697
MspA1I CmG'CkG
Cuts at: 0 454 463 855
Size: 454 9 392
Fragments arranged by size:
454 392 9
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 260 292 855
Size: 260 32 563
Fragments arranged by size:
563 260 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 644 676 855
Size: 644 32 179
Fragments arranged by size:
644 179 32
PstI C_TGCA'G
Cuts at: 0 462 468 855
Size: 462 6 387
Fragments arranged by size:
462 387 6
PvuII CAG'CTG
Cuts at: 0 454 463 855
Size: 454 9 392
Fragments arranged by size:
454 392 9
SacI G_AGCT'C
Cuts at: 0 750 855
Size: 750 105
SfcI C'TryA_G
Cuts at: 0 458 464 855
Size: 458 6 391
Fragments arranged by size:
458 391 6
SmlI C'TyrA_G
Cuts at: 0 202 855
Size: 202 653
XmnI GAAnn'nnTTC
Cuts at: 0 275 855
Size: 275 580
Enzymes that do cut:
AclI AfeI AhdI AlfI AloI AlwNI AvaI BanII
BbsI BciVI BglII BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspMI BstAPI
BstYI DraI EcoICRI Eco57MI FalI HaeII Hin4I MmeI
MslI MspA1I PpiI PsrI PstI PvuII SacI SfcI
SmlI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AgeI
AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EarI EciI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI