Restriction map for
pFN21AE5343
(Linear) (Six-base) MAPSORT of: KIEE5343.seq Check: 8007 from: 1 to: 810
KIEE5343.orf
With 182 enzymes: SgfI *
January 29, 2014 11:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 282 810
Size: 282 528
AflIII A'CryG_T
Cuts at: 0 167 169 810
Size: 167 2 641
Fragments arranged by size:
641 167 2
AleI CACnn'nnGTG
Cuts at: 0 170 810
Size: 170 640
ApaI G_GGCC'C
Cuts at: 0 286 810
Size: 286 524
AvaI C'yCGr_G
Cuts at: 0 225 286 761 810
Size: 225 61 475 49
Fragments arranged by size:
475 225 61 49
BanII G_rGCy'C
Cuts at: 0 232 286 810
Size: 232 54 524
Fragments arranged by size:
524 232 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 604 810
Size: 604 206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 351 385 810
Size: 351 34 425
Fragments arranged by size:
425 351 34
Bme1580I G_kGCm'C
Cuts at: 0 286 810
Size: 286 524
BmrI ACTGGGnnnn_n'
Cuts at: 0 207 810
Size: 207 603
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 676 810
Size: 676 134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 635 671 810
Size: 549 86 36 139
Fragments arranged by size:
549 139 86 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 787 810
Size: 787 23
BsaAI yAC'GTr
Cuts at: 0 170 810
Size: 170 640
BsaBI GATnn'nnATC
Cuts at: 0 301 810
Size: 301 509
BsaHI Gr'CG_yC
Cuts at: 0 410 810
Size: 410 400
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 398 428 613 643 810
Size: 398 30 185 30 167
Fragments arranged by size:
398 185 167 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 805 810
Size: 16 789 5
Fragments arranged by size:
789 16 5
BseYI C'CCAG_C
Cuts at: 0 398 402 482 810
Size: 398 4 80 328
Fragments arranged by size:
398 328 80 4
BsiEI CG_ry'CG
Cuts at: 0 267 810
Size: 267 543
BsiHKAI G_wGCw'C
Cuts at: 0 232 810
Size: 232 578
BsmBI CGTCTCn'nnnn_
Cuts at: 0 401 496 810
Size: 401 95 314
Fragments arranged by size:
401 314 95
Bsp1286I G_dGCh'C
Cuts at: 0 232 286 810
Size: 232 54 524
Fragments arranged by size:
524 232 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 282 681 810
Size: 117 165 399 129
Fragments arranged by size:
399 165 129 117
BsrBI CCG'CTC
Cuts at: 0 344 629 810
Size: 344 285 181
Fragments arranged by size:
344 285 181
BsrDI GCAATG_nn'
Cuts at: 0 369 810
Size: 369 441
BsrFI r'CCGG_y
Cuts at: 0 211 478 810
Size: 211 267 332
Fragments arranged by size:
332 267 211
BssSI C'ACGA_G
Cuts at: 0 345 810
Size: 345 465
BstXI CCAn_nnnn'nTGG
Cuts at: 0 172 810
Size: 172 638
Bsu36I CC'TnA_GG
Cuts at: 0 45 810
Size: 45 765
BtgI C'CryG_G
Cuts at: 0 542 602 696 749 810
Size: 542 60 94 53 61
Fragments arranged by size:
542 94 61 60 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 171 354 810
Size: 171 183 456
Fragments arranged by size:
456 183 171
BtsI GCAGTG_nn'
Cuts at: 0 216 558 810
Size: 216 342 252
Fragments arranged by size:
342 252 216
EaeI y'GGCC_r
Cuts at: 0 35 213 810
Size: 35 178 597
Fragments arranged by size:
597 178 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 759 810
Size: 759 51
EcoICRI GAG'CTC
Cuts at: 0 230 810
Size: 230 580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 635 671 810
Size: 549 86 36 139
Fragments arranged by size:
549 139 86 36
EcoO109I rG'GnC_Cy
Cuts at: 0 282 283 315 531 655 810
Size: 282 1 32 216 124 155
Fragments arranged by size:
282 216 155 124 32 1
HaeII r_GCGC'y
Cuts at: 0 398 810
Size: 398 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 398 430 608 613 640 645 810
Size: 398 32 178 5 27 5 165
Fragments arranged by size:
398 178 165 32 27 5 5
HindIII A'AGCT_T
Cuts at: 0 156 810
Size: 156 654
MslI CAynn'nnrTG
Cuts at: 0 170 810
Size: 170 640
MspA1I CmG'CkG
Cuts at: 0 116 402 439 810
Size: 116 286 37 371
Fragments arranged by size:
371 286 116 37
NaeI GCC'GGC
Cuts at: 0 213 810
Size: 213 597
NcoI C'CATG_G
Cuts at: 0 602 696 810
Size: 602 94 114
Fragments arranged by size:
602 114 94
NgoMIV G'CCGG_C
Cuts at: 0 211 810
Size: 211 599
PfoI T'CCnGG_A
Cuts at: 0 232 810
Size: 232 578
PmlI CAC'GTG
Cuts at: 0 170 810
Size: 170 640
PpuMI rG'GwC_Cy
Cuts at: 0 315 655 810
Size: 315 340 155
Fragments arranged by size:
340 315 155
PspOMI G'GGCC_C
Cuts at: 0 282 810
Size: 282 528
PspXI vC'TCGA_Gb
Cuts at: 0 761 810
Size: 761 49
PstI C_TGCA'G
Cuts at: 0 140 527 810
Size: 140 387 283
Fragments arranged by size:
387 283 140
PvuII CAG'CTG
Cuts at: 0 116 402 810
Size: 116 286 408
Fragments arranged by size:
408 286 116
SacI G_AGCT'C
Cuts at: 0 232 810
Size: 232 578
SexAI A'CCwGG_T
Cuts at: 0 242 810
Size: 242 568
SfcI C'TryA_G
Cuts at: 0 111 136 523 810
Size: 111 25 387 287
Fragments arranged by size:
387 287 111 25
SmlI C'TyrA_G
Cuts at: 0 225 761 810
Size: 225 536 49
Fragments arranged by size:
536 225 49
StyI C'CwwG_G
Cuts at: 0 602 696 810
Size: 602 94 114
Fragments arranged by size:
602 114 94
Tth111I GACn'n_nGTC
Cuts at: 0 412 810
Size: 412 398
XhoI C'TCGA_G
Cuts at: 0 225 761 810
Size: 225 536 49
Fragments arranged by size:
536 225 49
Enzymes that do cut:
AarI AflIII AleI ApaI AvaI BanII BbsI BcgI
Bme1580I BmrI BplI BpmI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstXI Bsu36I BtgI BtgZI
BtsI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HindIII MslI MspA1I NaeI NcoI NgoMIV PfoI PmlI
PpuMI PspOMI PspXI PstI PvuII SacI SexAI SfcI
SmlI StyI Tth111I XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I
BpuEI BsaWI BsgI BsiWI BsmI BspEI BspHI BsrGI
BssHII BstAPI BstBI BstEII BstYI BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EarI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MmeI MscI NarI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI XbaI XcmI XmaI XmnI ZraI