Restriction map for
pFN21AE5339
(Linear) (Six-base) MAPSORT of: KIEE5339.seq Check: 3967 from: 1 to: 792
KIEE5339.orf
With 182 enzymes: SgfI *
January 29, 2014 11:56 ..
AatII G_ACGT'C
Cuts at: 0 713 792
Size: 713 79
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 792
Size: 626 166
AlwNI CAG_nnn'CTG
Cuts at: 0 167 792
Size: 167 625
ApaLI G'TGCA_C
Cuts at: 0 109 792
Size: 109 683
ApoI r'AATT_y
Cuts at: 0 355 406 567 792
Size: 355 51 161 225
Fragments arranged by size:
355 225 161 51
AvaI C'yCGr_G
Cuts at: 0 8 792
Size: 8 784
BanI G'GyrC_C
Cuts at: 0 21 73 292 792
Size: 21 52 219 500
Fragments arranged by size:
500 219 52 21
BbeI G_GCGC'C
Cuts at: 0 25 77 296 792
Size: 25 52 219 496
Fragments arranged by size:
496 219 52 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 517 792
Size: 517 275
BglI GCCn_nnn'nGGC
Cuts at: 0 19 301 792
Size: 19 282 491
Fragments arranged by size:
491 282 19
Bme1580I G_kGCm'C
Cuts at: 0 113 268 792
Size: 113 155 524
Fragments arranged by size:
524 155 113
BmgBI CAC'GTC
Cuts at: 0 108 792
Size: 108 684
BmrI ACTGGGnnnn_n'
Cuts at: 0 352 661 792
Size: 352 309 131
Fragments arranged by size:
352 309 131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 142 792
Size: 126 16 650
Fragments arranged by size:
650 126 16
BsaHI Gr'CG_yC
Cuts at: 0 22 74 245 293 710 792
Size: 22 52 171 48 417 82
Fragments arranged by size:
417 171 82 52 48 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 382 792
Size: 28 354 410
Fragments arranged by size:
410 354 28
BsiHKAI G_wGCw'C
Cuts at: 0 113 792
Size: 113 679
Bsp1286I G_dGCh'C
Cuts at: 0 113 268 792
Size: 113 155 524
Fragments arranged by size:
524 155 113
BspHI T'CATG_A
Cuts at: 0 311 351 792
Size: 311 40 441
Fragments arranged by size:
441 311 40
BsrFI r'CCGG_y
Cuts at: 0 116 247 792
Size: 116 131 545
Fragments arranged by size:
545 131 116
BsrGI T'GTAC_A
Cuts at: 0 284 792
Size: 284 508
BstXI CCAn_nnnn'nTGG
Cuts at: 0 342 792
Size: 342 450
BstYI r'GATC_y
Cuts at: 0 209 792
Size: 209 583
BtgI C'CryG_G
Cuts at: 0 335 792
Size: 335 457
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 669 792
Size: 669 123
BtsI GCAGTG_nn'
Cuts at: 0 590 792
Size: 590 202
EarI CTCTTCn'nnn_
Cuts at: 0 196 792
Size: 196 596
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 202 792
Size: 202 590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 142 626 792
Size: 126 16 484 166
Fragments arranged by size:
484 166 126 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 792
Size: 397 395
HaeII r_GCGC'y
Cuts at: 0 25 77 296 792
Size: 25 52 219 496
Fragments arranged by size:
496 219 52 25
HincII GTy'rAC
Cuts at: 0 156 792
Size: 156 636
KasI G'GCGC_C
Cuts at: 0 21 73 292 792
Size: 21 52 219 500
Fragments arranged by size:
500 219 52 21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 755 792
Size: 501 254 37
Fragments arranged by size:
501 254 37
MspA1I CmG'CkG
Cuts at: 0 167 792
Size: 167 625
NaeI GCC'GGC
Cuts at: 0 118 249 792
Size: 118 131 543
Fragments arranged by size:
543 131 118
NarI GG'CG_CC
Cuts at: 0 22 74 293 792
Size: 22 52 219 499
Fragments arranged by size:
499 219 52 22
NcoI C'CATG_G
Cuts at: 0 335 792
Size: 335 457
NgoMIV G'CCGG_C
Cuts at: 0 116 247 792
Size: 116 131 545
Fragments arranged by size:
545 131 116
PasI CC'CwG_GG
Cuts at: 0 45 792
Size: 45 747
SapI GCTCTTCn'nnn_
Cuts at: 0 196 792
Size: 196 596
SfoI GGC'GCC
Cuts at: 0 23 75 294 792
Size: 23 52 219 498
Fragments arranged by size:
498 219 52 23
SgrAI Cr'CCGG_yG
Cuts at: 0 247 792
Size: 247 545
StyI C'CwwG_G
Cuts at: 0 197 335 792
Size: 197 138 457
Fragments arranged by size:
457 197 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 792
Size: 163 629
TatI w'GTAC_w
Cuts at: 0 284 792
Size: 284 508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 168 792
Size: 168 624
ZraI GAC'GTC
Cuts at: 0 711 792
Size: 711 81
Enzymes that do cut:
AatII AcuI AlwNI ApaLI ApoI AvaI BanI BbeI
BciVI BglI Bme1580I BmgBI BmrI BpmI BsaHI BseRI
BsiHKAI Bsp1286I BspHI BsrFI BsrGI BstXI BstYI BtgI
BtgZI BtsI EarI EciI Eco57MI EcoNI HaeII HincII
KasI MmeI MspA1I NaeI NarI NcoI NgoMIV PasI
SapI SfoI SgrAI StyI TaqII TatI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbsI BbvCI BcgI BclI
BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrDI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EaeI EagI EcoICRI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI
MfeI MluI MscI MslI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI