Restriction map for
pFN21AE5336
(Linear) (Six-base) MAPSORT of: KIEE5336.seq Check: 3576 from: 1 to: 804
KIEE5336.orf
With 182 enzymes: SgfI *
January 29, 2014 11:56 ..
AccI GT'mk_AC
Cuts at: 0 752 804
Size: 752 52
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 804
Size: 564 240
AflIII A'CryG_T
Cuts at: 0 158 172 804
Size: 158 14 632
Fragments arranged by size:
632 158 14
AleI CACnn'nnGTG
Cuts at: 0 760 804
Size: 760 44
AlwNI CAG_nnn'CTG
Cuts at: 0 528 804
Size: 528 276
AvaI C'yCGr_G
Cuts at: 0 83 758 804
Size: 83 675 46
Fragments arranged by size:
675 83 46
BamHI G'GATC_C
Cuts at: 0 292 804
Size: 292 512
BanI G'GyrC_C
Cuts at: 0 54 804
Size: 54 750
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 804
Size: 287 517
BglII A'GATC_T
Cuts at: 0 131 804
Size: 131 673
Bme1580I G_kGCm'C
Cuts at: 0 59 804
Size: 59 745
BmgBI CAC'GTC
Cuts at: 0 324 804
Size: 324 480
BmtI G_CTAG'C
Cuts at: 0 197 804
Size: 197 607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 453 804
Size: 453 351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 124 292 543 804
Size: 27 97 168 251 261
Fragments arranged by size:
261 251 168 97 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 804
Size: 133 671
BsaAI yAC'GTr
Cuts at: 0 161 173 554 804
Size: 161 12 381 250
Fragments arranged by size:
381 250 161 12
BsaHI Gr'CG_yC
Cuts at: 0 620 804
Size: 620 184
BsaWI w'CCGG_w
Cuts at: 0 646 804
Size: 646 158
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 110 804
Size: 110 694
BseYI C'CCAG_C
Cuts at: 0 58 476 607 804
Size: 58 418 131 197
Fragments arranged by size:
418 197 131 58
BsiHKAI G_wGCw'C
Cuts at: 0 559 804
Size: 559 245
BsmI GAATG_Cn'
Cuts at: 0 345 804
Size: 345 459
Bsp1286I G_dGCh'C
Cuts at: 0 59 559 804
Size: 59 500 245
Fragments arranged by size:
500 245 59
BsrDI GCAATG_nn'
Cuts at: 0 363 503 642 804
Size: 363 140 139 162
Fragments arranged by size:
363 162 140 139
BsrFI r'CCGG_y
Cuts at: 0 472 744 804
Size: 472 272 60
Fragments arranged by size:
472 272 60
BstAPI GCAn_nnn'nTGC
Cuts at: 0 528 804
Size: 528 276
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 804
Size: 414 390
BstYI r'GATC_y
Cuts at: 0 131 292 804
Size: 131 161 512
Fragments arranged by size:
512 161 131
BtgI C'CryG_G
Cuts at: 0 254 536 596 804
Size: 254 282 60 208
Fragments arranged by size:
282 254 208 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 629 804
Size: 629 175
BtsI GCAGTG_nn'
Cuts at: 0 209 219 804
Size: 209 10 585
Fragments arranged by size:
585 209 10
EaeI y'GGCC_r
Cuts at: 0 251 804
Size: 251 553
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 69 756 804
Size: 69 687 48
Fragments arranged by size:
687 69 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 124 292 543 564 804
Size: 27 97 168 251 21 240
Fragments arranged by size:
251 240 168 97 27 21
EcoO109I rG'GnC_Cy
Cuts at: 0 525 804
Size: 525 279
FspI TGC'GCA
Cuts at: 0 355 804
Size: 355 449
HindIII A'AGCT_T
Cuts at: 0 376 804
Size: 376 428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 804
Size: 650 154
MslI CAynn'nnrTG
Cuts at: 0 173 412 760 804
Size: 173 239 348 44
Fragments arranged by size:
348 239 173 44
MspA1I CmG'CkG
Cuts at: 0 47 119 801 804
Size: 47 72 682 3
Fragments arranged by size:
682 72 47 3
NcoI C'CATG_G
Cuts at: 0 596 804
Size: 596 208
NheI G'CTAG_C
Cuts at: 0 193 804
Size: 193 611
NspI r_CATG'y
Cuts at: 0 391 804
Size: 391 413
PfoI T'CCnGG_A
Cuts at: 0 559 804
Size: 559 245
PmlI CAC'GTG
Cuts at: 0 161 554 804
Size: 161 393 250
Fragments arranged by size:
393 250 161
PstI C_TGCA'G
Cuts at: 0 521 533 804
Size: 521 12 271
Fragments arranged by size:
521 271 12
PvuII CAG'CTG
Cuts at: 0 47 119 801 804
Size: 47 72 682 3
Fragments arranged by size:
682 72 47 3
SfcI C'TryA_G
Cuts at: 0 517 529 804
Size: 517 12 275
Fragments arranged by size:
517 275 12
SmaI CCC'GGG
Cuts at: 0 760 804
Size: 760 44
SmlI C'TyrA_G
Cuts at: 0 148 804
Size: 148 656
StyI C'CwwG_G
Cuts at: 0 449 596 804
Size: 449 147 208
Fragments arranged by size:
449 208 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 295 804
Size: 295 509
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 231 263 804
Size: 231 32 541
Fragments arranged by size:
541 231 32
XmaI C'CCGG_G
Cuts at: 0 758 804
Size: 758 46
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI AvaI BamHI BanI
BbsI BglII Bme1580I BmgBI BmtI BplI BpmI BpuEI
BsaAI BsaHI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I
BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI
EaeI EciI Eco57MI EcoO109I FspI HindIII MmeI MslI
MspA1I NcoI NheI NspI PfoI PmlI PstI PvuII
SfcI SmaI SmlI StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmrI Bpu10I BsaI BsaBI BsaXI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NotI NruI NsiI PacI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XcmI XhoI XmnI ZraI