(Linear) (Six-base) MAPSORT of: KIEE5334.seq  Check: 1384  from: 1  to: 783



KIEE5334.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     333     783 
   Size:        333     450
 
AhdI GACnn_n'nnGTC

Cuts at:      0     118     783 
   Size:        118     665
 
ApoI r'AATT_y

Cuts at:      0     125     431     783 
   Size:        125     306     352

  Fragments arranged by size: 

                 352     306     125
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     512     545     783 
   Size:        512      33     238

  Fragments arranged by size: 

                 512     238      33
 
BanII G_rGCy'C

Cuts at:      0     668     675     783 
   Size:        668       7     108

  Fragments arranged by size: 

                 668     108       7
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     397     783 
   Size:        397     386
 
BlpI GC'TnA_GC

Cuts at:      0     674     783 
   Size:        674     109
 
Bpu10I CC'TnA_GC

Cuts at:      0     688     783 
   Size:        688      95
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     439     783 
   Size:        439     344
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     225     783 
   Size:        225     558
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     356     386     783 
   Size:        356      30     397

  Fragments arranged by size: 

                 397     356      30
 
BseYI C'CCAG_C

Cuts at:      0     568     783 
   Size:        568     215
 
BsiHKAI G_wGCw'C

Cuts at:      0     668     783 
   Size:        668     115
 
Bsp1286I G_dGCh'C

Cuts at:      0     668     675     783 
   Size:        668       7     108

  Fragments arranged by size: 

                 668     108       7
 
BspHI T'CATG_A

Cuts at:      0     627     783 
   Size:        627     156
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     333     783 
   Size:        333     450
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      94     783 
   Size:         94     689
 
Bsu36I CC'TnA_GG

Cuts at:      0     116     783 
   Size:        116     667
 
BtgI C'CryG_G

Cuts at:      0     401     783 
   Size:        401     382
 
BtsI GCAGTG_nn'

Cuts at:      0      69     783 
   Size:         69     714
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     354     691     783 
   Size:        354     337      92

  Fragments arranged by size: 

                 354     337      92
 
EarI CTCTTCn'nnn_

Cuts at:      0     646     783 
   Size:        646     137
 
EcoICRI GAG'CTC

Cuts at:      0     666     783 
   Size:        666     117
 
EcoRI G'AATT_C

Cuts at:      0     125     783 
   Size:        125     658
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     320     352     356     388     705     737 
   Size:        104      32     184      32       4      32     317      32

Cuts at:    737     783 
   Size:         46

  Fragments arranged by size: 

                 317     184     104      46      32      32      32      32
                   4
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     342     783 
   Size:        342     441
 
PfoI T'CCnGG_A

Cuts at:      0     602     783 
   Size:        602     181
 
SacI G_AGCT'C

Cuts at:      0     668     783 
   Size:        668     115
 
SapI GCTCTTCn'nnn_

Cuts at:      0     646     783 
   Size:        646     137
 
SfcI C'TryA_G

Cuts at:      0     527     783 
   Size:        527     256
 
SmlI C'TyrA_G

Cuts at:      0     418     783 
   Size:        418     365
 
XmnI GAAnn'nnTTC

Cuts at:      0     457     783 
   Size:        457     326
 

 Enzymes that do cut:

     AarI     AhdI     ApoI     BaeI    BanII    BciVI     BlpI   Bpu10I
    BpuEI     BsaI    BsaXI    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI
   BstAPI   Bsu36I     BtgI     BtsI     DrdI     EarI  EcoICRI    EcoRI
    Hin4I     MmeI     PfoI     SacI     SapI     SfcI     SmlI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI
     EagI     EciI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI