Restriction map for
pFN21AE5332
(Linear) (Six-base) MAPSORT of: KIEE5332.seq Check: 6997 from: 1 to: 870
KIEE5332.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 280 870
Size: 241 39 590
Fragments arranged by size:
590 241 39
AflIII A'CryG_T
Cuts at: 0 460 870
Size: 460 410
AleI CACnn'nnGTG
Cuts at: 0 307 870
Size: 307 563
ApaI G_GGCC'C
Cuts at: 0 255 870
Size: 255 615
ApoI r'AATT_y
Cuts at: 0 121 419 870
Size: 121 298 451
Fragments arranged by size:
451 298 121
BamHI G'GATC_C
Cuts at: 0 432 870
Size: 432 438
BanI G'GyrC_C
Cuts at: 0 358 870
Size: 358 512
BanII G_rGCy'C
Cuts at: 0 255 870
Size: 255 615
BbsI GAAGACnn'nnnn_
Cuts at: 0 221 870
Size: 221 649
BbvCI CC'TCA_GC
Cuts at: 0 470 870
Size: 470 400
BclI T'GATC_A
Cuts at: 0 344 870
Size: 344 526
Bme1580I G_kGCm'C
Cuts at: 0 255 870
Size: 255 615
BmrI ACTGGGnnnn_n'
Cuts at: 0 121 870
Size: 121 749
Bpu10I CC'TnA_GC
Cuts at: 0 470 870
Size: 470 400
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 870
Size: 259 30 581
Fragments arranged by size:
581 259 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 574 870
Size: 574 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 275 609 870
Size: 137 138 334 261
Fragments arranged by size:
334 261 138 137
BsmI GAATG_Cn'
Cuts at: 0 121 870
Size: 121 749
BsmBI CGTCTCn'nnnn_
Cuts at: 0 859 870
Size: 859 11
Bsp1286I G_dGCh'C
Cuts at: 0 255 870
Size: 255 615
BspHI T'CATG_A
Cuts at: 0 26 870
Size: 26 844
BstYI r'GATC_y
Cuts at: 0 432 642 870
Size: 432 210 228
Fragments arranged by size:
432 228 210
Bsu36I CC'TnA_GG
Cuts at: 0 647 870
Size: 647 223
BtsI GCAGTG_nn'
Cuts at: 0 513 870
Size: 513 357
DrdI GACnn_nn'nnGTC
Cuts at: 0 517 870
Size: 517 353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 280 870
Size: 241 39 590
Fragments arranged by size:
590 241 39
EcoO109I rG'GnC_Cy
Cuts at: 0 75 251 870
Size: 75 176 619
Fragments arranged by size:
619 176 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 627 659 817 849 870
Size: 259 32 336 32 158 32 21
Fragments arranged by size:
336 259 158 32 32 32 21
HindIII A'AGCT_T
Cuts at: 0 565 870
Size: 565 305
MfeI C'AATT_G
Cuts at: 0 110 870
Size: 110 760
MslI CAynn'nnrTG
Cuts at: 0 307 870
Size: 307 563
MspA1I CmG'CkG
Cuts at: 0 338 664 870
Size: 338 326 206
Fragments arranged by size:
338 326 206
NspI r_CATG'y
Cuts at: 0 464 870
Size: 464 406
PasI CC'CwG_GG
Cuts at: 0 279 870
Size: 279 591
PciI A'CATG_T
Cuts at: 0 460 870
Size: 460 410
PpuMI rG'GwC_Cy
Cuts at: 0 75 870
Size: 75 795
PspOMI G'GGCC_C
Cuts at: 0 251 870
Size: 251 619
PvuII CAG'CTG
Cuts at: 0 664 870
Size: 664 206
SfcI C'TryA_G
Cuts at: 0 240 870
Size: 240 630
StyI C'CwwG_G
Cuts at: 0 71 482 750 870
Size: 71 411 268 120
Fragments arranged by size:
411 268 120 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 492 524 870
Size: 492 32 346
Fragments arranged by size:
492 346 32
XmnI GAAnn'nnTTC
Cuts at: 0 568 870
Size: 568 302
Enzymes that do cut:
AcuI AflIII AleI ApaI ApoI BamHI BanI BanII
BbsI BbvCI BclI Bme1580I BmrI Bpu10I BsaXI BseRI
BsgI BsmI BsmBI Bsp1286I BspHI BstYI Bsu36I BtsI
DrdI Eco57MI EcoO109I Hin4I HindIII MfeI MslI MspA1I
NspI PasI PciI PpuMI PspOMI PvuII SfcI StyI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI
CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI ZraI