Restriction map for
pFN21AE5330
(Linear) (Six-base) MAPSORT of: KIEE5330.seq Check: 3194 from: 1 to: 807
KIEE5330.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 178 562 640 807
Size: 178 384 78 167
Fragments arranged by size:
384 178 167 78
Acc65I G'GTAC_C
Cuts at: 0 161 807
Size: 161 646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 807
Size: 538 269
AleI CACnn'nnGTG
Cuts at: 0 28 807
Size: 28 779
ApaLI G'TGCA_C
Cuts at: 0 319 807
Size: 319 488
BanI G'GyrC_C
Cuts at: 0 161 436 807
Size: 161 275 371
Fragments arranged by size:
371 275 161
BanII G_rGCy'C
Cuts at: 0 42 504 807
Size: 42 462 303
Fragments arranged by size:
462 303 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 595 807
Size: 595 212
Bme1580I G_kGCm'C
Cuts at: 0 323 807
Size: 323 484
BmgBI CAC'GTC
Cuts at: 0 713 807
Size: 713 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 303 807
Size: 303 504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 216 543 807
Size: 56 160 327 264
Fragments arranged by size:
327 264 160 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 60 135 351 768 807
Size: 60 75 216 417 39
Fragments arranged by size:
417 216 75 60 39
BsaWI w'CCGG_w
Cuts at: 0 646 807
Size: 646 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 319 331 349 361 376 406 628 658
Size: 319 12 18 12 15 30 222 30
Cuts at: 658 730 760 807
Size: 72 30 47
Fragments arranged by size:
319 222 72 47 30 30 30 18
15 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 219 807
Size: 219 588
BseYI C'CCAG_C
Cuts at: 0 197 252 476 607 807
Size: 197 55 224 131 200
Fragments arranged by size:
224 200 197 131 55
BsiEI CG_ry'CG
Cuts at: 0 681 807
Size: 681 126
BsiHKAI G_wGCw'C
Cuts at: 0 323 807
Size: 323 484
BsmBI CGTCTCn'nnnn_
Cuts at: 0 396 807
Size: 396 411
Bsp1286I G_dGCh'C
Cuts at: 0 42 323 504 807
Size: 42 281 181 303
Fragments arranged by size:
303 281 181 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 178 562 640 807
Size: 117 61 384 78 167
Fragments arranged by size:
384 167 117 78 61
BsrDI GCAATG_nn'
Cuts at: 0 362 807
Size: 362 445
BsrFI r'CCGG_y
Cuts at: 0 433 527 680 807
Size: 433 94 153 127
Fragments arranged by size:
433 153 127 94
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 807
Size: 30 777
BstYI r'GATC_y
Cuts at: 0 236 407 807
Size: 236 171 400
Fragments arranged by size:
400 236 171
BtsI GCAGTG_nn'
Cuts at: 0 216 807
Size: 216 591
EaeI y'GGCC_r
Cuts at: 0 153 293 678 807
Size: 153 140 385 129
Fragments arranged by size:
385 153 140 129
EagI C'GGCC_G
Cuts at: 0 678 807
Size: 678 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 216 538 543 807
Size: 56 160 322 5 264
Fragments arranged by size:
322 264 160 56 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 520 807
Size: 520 287
EcoO109I rG'GnC_Cy
Cuts at: 0 8 179 201 807
Size: 8 171 22 606
Fragments arranged by size:
606 171 22 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 807
Size: 319 32 456
Fragments arranged by size:
456 319 32
KpnI G_GTAC'C
Cuts at: 0 165 807
Size: 165 642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 791 807
Size: 362 429 16
Fragments arranged by size:
429 362 16
MscI TGG'CCA
Cuts at: 0 155 807
Size: 155 652
MslI CAynn'nnrTG
Cuts at: 0 28 807
Size: 28 779
MspA1I CmG'CkG
Cuts at: 0 116 197 414 657 807
Size: 116 81 217 243 150
Fragments arranged by size:
243 217 150 116 81
NaeI GCC'GGC
Cuts at: 0 435 807
Size: 435 372
NgoMIV G'CCGG_C
Cuts at: 0 433 807
Size: 433 374
PflMI CCAn_nnn'nTGG
Cuts at: 0 116 197 807
Size: 116 81 610
Fragments arranged by size:
610 116 81
PfoI T'CCnGG_A
Cuts at: 0 232 807
Size: 232 575
PpuMI rG'GwC_Cy
Cuts at: 0 8 179 201 807
Size: 8 171 22 606
Fragments arranged by size:
606 171 22 8
PstI C_TGCA'G
Cuts at: 0 140 491 807
Size: 140 351 316
Fragments arranged by size:
351 316 140
PvuII CAG'CTG
Cuts at: 0 116 197 414 807
Size: 116 81 217 393
Fragments arranged by size:
393 217 116 81
SanDI GG'GwC_CC
Cuts at: 0 179 807
Size: 179 628
SexAI A'CCwGG_T
Cuts at: 0 341 807
Size: 341 466
SfcI C'TryA_G
Cuts at: 0 136 487 807
Size: 136 351 320
Fragments arranged by size:
351 320 136
StyI C'CwwG_G
Cuts at: 0 720 807
Size: 720 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 705 807
Size: 705 102
TatI w'GTAC_w
Cuts at: 0 140 807
Size: 140 667
Enzymes that do cut:
AarI Acc65I AcuI AleI ApaLI BanI BanII BbsI
Bme1580I BmgBI BplI BpmI BsaI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI
BstXI BstYI BtsI EaeI EagI Eco57MI EcoNI EcoO109I
Hin4I KpnI MmeI MscI MslI MspA1I NaeI NgoMIV
PflMI PfoI PpuMI PstI PvuII SanDI SexAI SfcI
StyI TaqII TatI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsgI BsiWI BsmI BspEI
BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI