Restriction map for
pFN21AE5328
(Linear) (Six-base) MAPSORT of: KIEE5328.seq Check: 194 from: 1 to: 816
KIEE5328.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 816
Size: 272 544
AhdI GACnn_n'nnGTC
Cuts at: 0 24 816
Size: 24 792
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 383 415 816
Size: 383 32 401
Fragments arranged by size:
401 383 32
AlwNI CAG_nnn'CTG
Cuts at: 0 418 816
Size: 418 398
AvaI C'yCGr_G
Cuts at: 0 84 816
Size: 84 732
BanI G'GyrC_C
Cuts at: 0 43 816
Size: 43 773
BanII G_rGCy'C
Cuts at: 0 698 816
Size: 698 118
BbeI G_GCGC'C
Cuts at: 0 47 816
Size: 47 769
BbsI GAAGACnn'nnnn_
Cuts at: 0 261 816
Size: 261 555
BfrBI ATG'CAT
Cuts at: 0 727 816
Size: 727 89
Bpu10I CC'TnA_GC
Cuts at: 0 692 757 816
Size: 692 65 59
Fragments arranged by size:
692 65 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 530 816
Size: 530 286
BsaBI GATnn'nnATC
Cuts at: 0 606 816
Size: 606 210
BsaHI Gr'CG_yC
Cuts at: 0 44 816
Size: 44 772
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 617 816
Size: 617 199
BseYI C'CCAG_C
Cuts at: 0 127 678 816
Size: 127 551 138
Fragments arranged by size:
551 138 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 816
Size: 38 778
BsiEI CG_ry'CG
Cuts at: 0 21 816
Size: 21 795
BsiHKAI G_wGCw'C
Cuts at: 0 98 301 368 816
Size: 98 203 67 448
Fragments arranged by size:
448 203 98 67
Bsp1286I G_dGCh'C
Cuts at: 0 98 301 368 698 816
Size: 98 203 67 330 118
Fragments arranged by size:
330 203 118 98 67
BspHI T'CATG_A
Cuts at: 0 402 816
Size: 402 414
BstEII G'GTnAC_C
Cuts at: 0 423 816
Size: 423 393
Bsu36I CC'TnA_GG
Cuts at: 0 356 816
Size: 356 460
BtgI C'CryG_G
Cuts at: 0 39 816
Size: 39 777
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 754 816
Size: 754 62
DraI TTT'AAA
Cuts at: 0 497 816
Size: 497 319
EaeI y'GGCC_r
Cuts at: 0 75 816
Size: 75 741
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 816
Size: 272 544
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 816
Size: 355 461
EcoO109I rG'GnC_Cy
Cuts at: 0 281 559 816
Size: 281 278 257
Fragments arranged by size:
281 278 257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 285 664 816
Size: 285 379 152
Fragments arranged by size:
379 285 152
HaeII r_GCGC'y
Cuts at: 0 39 47 816
Size: 39 8 769
Fragments arranged by size:
769 39 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 163 195 816
Size: 10 32 121 32 621
Fragments arranged by size:
621 121 32 32 10
KasI G'GCGC_C
Cuts at: 0 43 816
Size: 43 773
MfeI C'AATT_G
Cuts at: 0 291 816
Size: 291 525
MslI CAynn'nnrTG
Cuts at: 0 722 816
Size: 722 94
MspA1I CmG'CkG
Cuts at: 0 245 816
Size: 245 571
NarI GG'CG_CC
Cuts at: 0 44 816
Size: 44 772
NsiI A_TGCA'T
Cuts at: 0 729 816
Size: 729 87
PasI CC'CwG_GG
Cuts at: 0 160 556 816
Size: 160 396 260
Fragments arranged by size:
396 260 160
PfoI T'CCnGG_A
Cuts at: 0 344 816
Size: 344 472
PpuMI rG'GwC_Cy
Cuts at: 0 281 816
Size: 281 535
SfoI GGC'GCC
Cuts at: 0 45 816
Size: 45 771
SmaI CCC'GGG
Cuts at: 0 86 816
Size: 86 730
SspI AAT'ATT
Cuts at: 0 666 816
Size: 666 150
StyI C'CwwG_G
Cuts at: 0 47 816
Size: 47 769
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 630 816
Size: 630 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 291 323 379 411 816
Size: 291 32 56 32 405
Fragments arranged by size:
405 291 56 32 32
XmaI C'CCGG_G
Cuts at: 0 84 816
Size: 84 732
Enzymes that do cut:
AcuI AhdI AloI AlwNI AvaI BanI BanII BbeI
BbsI BfrBI Bpu10I BsaI BsaBI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BstEII Bsu36I BtgI
BtgZI DraI EaeI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI MfeI MslI MspA1I NarI NsiI PasI
PfoI PpuMI SfoI SmaI SspI StyI TaqII TstI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaAI BsaWI BsaXI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtsI ClaI CspCI
DraIII DrdI EagI EarI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MluI
MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TatI Tth111I XbaI XcmI
XhoI XmnI ZraI