(Linear) (Six-base) MAPSORT of: KIEE5320.seq  Check: 1641  from: 1  to: 873



KIEE5320.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     448     873 
   Size:        448     425
 
AccI GT'mk_AC

Cuts at:      0     198     873 
   Size:        198     675
 
Acc65I G'GTAC_C

Cuts at:      0     463     873 
   Size:        463     410
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550     873 
   Size:        550     323
 
AlwNI CAG_nnn'CTG

Cuts at:      0     600     873 
   Size:        600     273
 
ApaI G_GGCC'C

Cuts at:      0     450     873 
   Size:        450     423
 
ApoI r'AATT_y

Cuts at:      0     185     477     873 
   Size:        185     292     396

  Fragments arranged by size: 

                 396     292     185
 
AvaI C'yCGr_G

Cuts at:      0     449     873 
   Size:        449     424
 
BanI G'GyrC_C

Cuts at:      0     463     873 
   Size:        463     410
 
BanII G_rGCy'C

Cuts at:      0     450     873 
   Size:        450     423
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     705     873 
   Size:        705     168
 
Bme1580I G_kGCm'C

Cuts at:      0     450     873 
   Size:        450     423
 
BmgBI CAC'GTC

Cuts at:      0      44     873 
   Size:         44     829
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     684     714     873 
   Size:        684      30     159

  Fragments arranged by size: 

                 684     159      30
 
BseYI C'CCAG_C

Cuts at:      0     561     593     873 
   Size:        561      32     280

  Fragments arranged by size: 

                 561     280      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     476     873 
   Size:        476     397
 
Bsp1286I G_dGCh'C

Cuts at:      0     450     873 
   Size:        450     423
 
BspHI T'CATG_A

Cuts at:      0     428     873 
   Size:        428     445
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     448     873 
   Size:        448     425
 
BsrFI r'CCGG_y

Cuts at:      0     819     873 
   Size:        819      54
 
BstEII G'GTnAC_C

Cuts at:      0     552     873 
   Size:        552     321
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     175     873 
   Size:        175     698
 
BtgI C'CryG_G

Cuts at:      0      10     873 
   Size:         10     863
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      51     873 
   Size:         51     822
 
EaeI y'GGCC_r

Cuts at:      0     650     873 
   Size:        650     223
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550     873 
   Size:        550     323
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     684     716     817     849     873 
   Size:        684      32     101      32      24

  Fragments arranged by size: 

                 684     101      32      32      24
 
KpnI G_GTAC'C

Cuts at:      0     467     873 
   Size:        467     406
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     703     873 
   Size:        703     170
 
MscI TGG'CCA

Cuts at:      0     652     873 
   Size:        652     221
 
MslI CAynn'nnrTG

Cuts at:      0      46     126     873 
   Size:         46      80     747

  Fragments arranged by size: 

                 747      80      46
 
MspA1I CmG'CkG

Cuts at:      0     118     873 
   Size:        118     755
 
NcoI C'CATG_G

Cuts at:      0      10     873 
   Size:         10     863
 
NspI r_CATG'y

Cuts at:      0     397     873 
   Size:        397     476
 
PasI CC'CwG_GG

Cuts at:      0     306     873 
   Size:        306     567
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     368     600     873 
   Size:        368     232     273

  Fragments arranged by size: 

                 368     273     232
 
PsiI TTA'TAA

Cuts at:      0     472     873 
   Size:        472     401
 
PspOMI G'GGCC_C

Cuts at:      0     446     873 
   Size:        446     427
 
PvuII CAG'CTG

Cuts at:      0     118     873 
   Size:        118     755
 
StyI C'CwwG_G

Cuts at:      0      10     548     873 
   Size:         10     538     325

  Fragments arranged by size: 

                 538     325      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     607     873 
   Size:        607     266
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     175     645     873 
   Size:        175     470     228

  Fragments arranged by size: 

                 470     228     175
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII    BciVI Bme1580I    BmgBI    BsaXI    BseYI     BsgI
 Bsp1286I    BspHI    BspMI    BsrFI   BstEII    BstXI     BtgI    BtgZI
     EaeI  Eco57MI    Hin4I     KpnI     MmeI     MscI     MslI   MspA1I
     NcoI     NspI     PasI    PflMI     PsiI   PspOMI    PvuII     StyI
    TaqII     XcmI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI