Restriction map for
pFN21AE5317
(Linear) (Six-base) MAPSORT of: KIEE5317.seq Check: 5541 from: 1 to: 867
KIEE5317.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AccI GT'mk_AC
Cuts at: 0 79 637 867
Size: 79 558 230
Fragments arranged by size:
558 230 79
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 867
Size: 450 417
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 197 867
Size: 197 670
ApoI r'AATT_y
Cuts at: 0 692 778 867
Size: 692 86 89
Fragments arranged by size:
692 89 86
BanII G_rGCy'C
Cuts at: 0 397 867
Size: 397 470
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 330 364 867
Size: 330 34 503
Fragments arranged by size:
503 330 34
BglI GCCn_nnn'nGGC
Cuts at: 0 117 867
Size: 117 750
Bme1580I G_kGCm'C
Cuts at: 0 186 867
Size: 186 681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 350 867
Size: 208 142 517
Fragments arranged by size:
517 208 142
Bpu10I CC'TnA_GC
Cuts at: 0 35 485 867
Size: 35 450 382
Fragments arranged by size:
450 382 35
BsaAI yAC'GTr
Cuts at: 0 700 867
Size: 700 167
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 401 431 867
Size: 401 30 436
Fragments arranged by size:
436 401 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 314 867
Size: 205 109 553
Fragments arranged by size:
553 205 109
BseYI C'CCAG_C
Cuts at: 0 474 570 867
Size: 474 96 297
Fragments arranged by size:
474 297 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 867
Size: 227 640
Bsp1286I G_dGCh'C
Cuts at: 0 186 397 867
Size: 186 211 470
Fragments arranged by size:
470 211 186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 649 867
Size: 649 218
BsrBI CCG'CTC
Cuts at: 0 680 867
Size: 680 187
BsrDI GCAATG_nn'
Cuts at: 0 231 348 867
Size: 231 117 519
Fragments arranged by size:
519 231 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 221 867
Size: 221 646
BstZ17I GTA'TAC
Cuts at: 0 638 867
Size: 638 229
BtgI C'CryG_G
Cuts at: 0 428 867
Size: 428 439
DraIII CAC_nnn'GTG
Cuts at: 0 21 867
Size: 21 846
EaeI y'GGCC_r
Cuts at: 0 68 425 707 729 867
Size: 68 357 282 22 138
Fragments arranged by size:
357 282 138 68 22
EarI CTCTTCn'nnn_
Cuts at: 0 146 764 867
Size: 146 618 103
Fragments arranged by size:
618 146 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 547 867
Size: 547 320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 350 450 867
Size: 208 142 100 417
Fragments arranged by size:
417 208 142 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 140 867
Size: 140 727
EcoO109I rG'GnC_Cy
Cuts at: 0 661 867
Size: 661 206
EcoRI G'AATT_C
Cuts at: 0 692 778 867
Size: 692 86 89
Fragments arranged by size:
692 89 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 837 867
Size: 837 30
HincII GTy'rAC
Cuts at: 0 577 867
Size: 577 290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 867
Size: 738 129
MscI TGG'CCA
Cuts at: 0 70 427 709 731 867
Size: 70 357 282 22 136
Fragments arranged by size:
357 282 136 70 22
MspA1I CmG'CkG
Cuts at: 0 478 831 867
Size: 478 353 36
Fragments arranged by size:
478 353 36
PmlI CAC'GTG
Cuts at: 0 700 867
Size: 700 167
PpuMI rG'GwC_Cy
Cuts at: 0 661 867
Size: 661 206
PstI C_TGCA'G
Cuts at: 0 360 867
Size: 360 507
PvuII CAG'CTG
Cuts at: 0 478 867
Size: 478 389
SexAI A'CCwGG_T
Cuts at: 0 73 867
Size: 73 794
SfcI C'TryA_G
Cuts at: 0 80 356 867
Size: 80 276 511
Fragments arranged by size:
511 276 80
TatI w'GTAC_w
Cuts at: 0 209 642 867
Size: 209 433 225
Fragments arranged by size:
433 225 209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 401 433 867
Size: 401 32 434
Fragments arranged by size:
434 401 32
Enzymes that do cut:
AccI AcuI AlfI ApoI BanII BcgI BglI Bme1580I
BpmI Bpu10I BsaAI BsaXI BseRI BseYI BsgI Bsp1286I
BspMI BsrBI BsrDI BstAPI BstZ17I BtgI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII
MmeI MscI MspA1I PmlI PpuMI PstI PvuII SexAI
SfcI TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstYI Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EagI EcoICRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI