Restriction map for
pFN21AE5314
(Linear) (Six-base) MAPSORT of: KIEE5314.seq Check: 7825 from: 1 to: 864
KIEE5314.orf
With 182 enzymes: SgfI *
December 5, 2014 13:00 ..
ApoI r'AATT_y
Cuts at: 0 346 864
Size: 346 518
AvaI C'yCGr_G
Cuts at: 0 41 864
Size: 41 823
BanI G'GyrC_C
Cuts at: 0 5 139 151 197 864
Size: 5 134 12 46 667
Fragments arranged by size:
667 134 46 12 5
BbeI G_GCGC'C
Cuts at: 0 9 155 201 864
Size: 9 146 46 663
Fragments arranged by size:
663 146 46 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 343 864
Size: 343 521
BglI GCCn_nnn'nGGC
Cuts at: 0 118 160 864
Size: 118 42 704
Fragments arranged by size:
704 118 42
BmgBI CAC'GTC
Cuts at: 0 95 864
Size: 95 769
BmrI ACTGGGnnnn_n'
Cuts at: 0 431 864
Size: 431 433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 864
Size: 391 473
BsaHI Gr'CG_yC
Cuts at: 0 6 121 152 198 864
Size: 6 115 31 46 666
Fragments arranged by size:
666 115 46 31 6
BseYI C'CCAG_C
Cuts at: 0 53 864
Size: 53 811
BsrFI r'CCGG_y
Cuts at: 0 163 864
Size: 163 701
BtgI C'CryG_G
Cuts at: 0 116 251 278 764 864
Size: 116 135 27 486 100
Fragments arranged by size:
486 135 116 100 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 225 864
Size: 225 639
EaeI y'GGCC_r
Cuts at: 0 110 212 864
Size: 110 102 652
Fragments arranged by size:
652 110 102
EarI CTCTTCn'nnn_
Cuts at: 0 488 864
Size: 488 376
EcoO109I rG'GnC_Cy
Cuts at: 0 62 864
Size: 62 802
HaeII r_GCGC'y
Cuts at: 0 9 155 201 864
Size: 9 146 46 663
Fragments arranged by size:
663 146 46 9
KasI G'GCGC_C
Cuts at: 0 5 151 197 864
Size: 5 146 46 667
Fragments arranged by size:
667 146 46 5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 864
Size: 615 249
MscI TGG'CCA
Cuts at: 0 214 864
Size: 214 650
MslI CAynn'nnrTG
Cuts at: 0 244 864
Size: 244 620
MspA1I CmG'CkG
Cuts at: 0 28 35 84 253 864
Size: 28 7 49 169 611
Fragments arranged by size:
611 169 49 28 7
NaeI GCC'GGC
Cuts at: 0 165 864
Size: 165 699
NarI GG'CG_CC
Cuts at: 0 6 152 198 864
Size: 6 146 46 666
Fragments arranged by size:
666 146 46 6
NcoI C'CATG_G
Cuts at: 0 116 278 764 864
Size: 116 162 486 100
Fragments arranged by size:
486 162 116 100
NdeI CA'TA_TG
Cuts at: 0 806 864
Size: 806 58
NgoMIV G'CCGG_C
Cuts at: 0 163 864
Size: 163 701
NspI r_CATG'y
Cuts at: 0 682 864
Size: 682 182
PflMI CCAn_nnn'nTGG
Cuts at: 0 221 864
Size: 221 643
PpuMI rG'GwC_Cy
Cuts at: 0 62 864
Size: 62 802
PshAI GACnn'nnGTC
Cuts at: 0 424 864
Size: 424 440
PsiI TTA'TAA
Cuts at: 0 760 864
Size: 760 104
PstI C_TGCA'G
Cuts at: 0 51 864
Size: 51 813
SacII CC_GC'GG
Cuts at: 0 254 864
Size: 254 610
SanDI GG'GwC_CC
Cuts at: 0 62 864
Size: 62 802
SfcI C'TryA_G
Cuts at: 0 47 864
Size: 47 817
SfoI GGC'GCC
Cuts at: 0 7 153 199 864
Size: 7 146 46 665
Fragments arranged by size:
665 146 46 7
SmlI C'TyrA_G
Cuts at: 0 370 864
Size: 370 494
StyI C'CwwG_G
Cuts at: 0 116 278 764 864
Size: 116 162 486 100
Fragments arranged by size:
486 162 116 100
XmnI GAAnn'nnTTC
Cuts at: 0 699 864
Size: 699 165
Enzymes that do cut:
ApoI AvaI BanI BbeI BbsI BglI BmgBI BmrI
BpuEI BsaHI BseYI BsrFI BtgI BtgZI EaeI EarI
EcoO109I HaeII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PflMI PpuMI PshAI
PsiI PstI SacII SanDI SfcI SfoI SmlI StyI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI