Restriction map for
pFN21AE5313
(Linear) (Six-base) MAPSORT of: KIEE5313.seq Check: 622 from: 1 to: 882
KIEE5313.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AccI GT'mk_AC
Cuts at: 0 131 882
Size: 131 751
AflIII A'CryG_T
Cuts at: 0 117 814 882
Size: 117 697 68
Fragments arranged by size:
697 117 68
AlwNI CAG_nnn'CTG
Cuts at: 0 585 882
Size: 585 297
ApaLI G'TGCA_C
Cuts at: 0 527 882
Size: 527 355
ApoI r'AATT_y
Cuts at: 0 373 882
Size: 373 509
BciVI GTATCCnnnnn_n'
Cuts at: 0 226 882
Size: 226 656
Bme1580I G_kGCm'C
Cuts at: 0 531 882
Size: 531 351
BmgBI CAC'GTC
Cuts at: 0 815 882
Size: 815 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 672 882
Size: 672 210
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 72 882
Size: 72 810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 882
Size: 186 696
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 882
Size: 5 877
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 828 858 882
Size: 184 30 614 30 24
Fragments arranged by size:
614 184 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 635 882
Size: 635 247
BseYI C'CCAG_C
Cuts at: 0 267 343 882
Size: 267 76 539
Fragments arranged by size:
539 267 76
BsiHKAI G_wGCw'C
Cuts at: 0 531 882
Size: 531 351
BsmI GAATG_Cn'
Cuts at: 0 73 882
Size: 73 809
Bsp1286I G_dGCh'C
Cuts at: 0 531 882
Size: 531 351
BspHI T'CATG_A
Cuts at: 0 351 629 776 882
Size: 351 278 147 106
Fragments arranged by size:
351 278 147 106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 323 882
Size: 323 559
BsrGI T'GTAC_A
Cuts at: 0 120 817 882
Size: 120 697 65
Fragments arranged by size:
697 120 65
BstXI CCAn_nnnn'nTGG
Cuts at: 0 42 748 882
Size: 42 706 134
Fragments arranged by size:
706 134 42
Bsu36I CC'TnA_GG
Cuts at: 0 611 882
Size: 611 271
BtgI C'CryG_G
Cuts at: 0 541 882
Size: 541 341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 882
Size: 186 696
EcoNI CCTnn'n_nnAGG
Cuts at: 0 20 647 882
Size: 20 627 235
Fragments arranged by size:
627 235 20
EcoRI G'AATT_C
Cuts at: 0 373 882
Size: 373 509
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 184 216 828 860 882
Size: 3 32 149 32 612 32 22
Fragments arranged by size:
612 149 32 32 32 22 3
HincII GTy'rAC
Cuts at: 0 132 882
Size: 132 750
MslI CAynn'nnrTG
Cuts at: 0 40 628 882
Size: 40 588 254
Fragments arranged by size:
588 254 40
MspA1I CmG'CkG
Cuts at: 0 163 882
Size: 163 719
NdeI CA'TA_TG
Cuts at: 0 125 144 321 882
Size: 125 19 177 561
Fragments arranged by size:
561 177 125 19
NspI r_CATG'y
Cuts at: 0 121 882
Size: 121 761
PciI A'CATG_T
Cuts at: 0 117 882
Size: 117 765
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 650 682 882
Size: 650 32 200
Fragments arranged by size:
650 200 32
SalI G'TCGA_C
Cuts at: 0 130 882
Size: 130 752
SmlI C'TyrA_G
Cuts at: 0 20 882
Size: 20 862
StyI C'CwwG_G
Cuts at: 0 532 882
Size: 532 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 796 882
Size: 82 714 86
Fragments arranged by size:
714 86 82
TatI w'GTAC_w
Cuts at: 0 50 120 817 882
Size: 50 70 697 65
Fragments arranged by size:
697 70 65 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 510 542 882
Size: 510 32 340
Fragments arranged by size:
510 340 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 226 748 766 882
Size: 226 522 18 116
Fragments arranged by size:
522 226 116 18
XmnI GAAnn'nnTTC
Cuts at: 0 511 882
Size: 511 371
Enzymes that do cut:
AccI AflIII AlwNI ApaLI ApoI BciVI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrGI BstXI Bsu36I BtgI
Eco57MI EcoNI EcoRI Hin4I HincII MslI MspA1I NdeI
NspI PciI PpiI SalI SmlI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI BmtI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI
BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
ZraI