Restriction map for
pFN21AE5312
(Linear) (Six-base) MAPSORT of: KIEE5312.seq Check: 9750 from: 1 to: 873
KIEE5312.orf
With 182 enzymes: SgfI *
December 5, 2014 13:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 797 873
Size: 487 310 76
Fragments arranged by size:
487 310 76
AhdI GACnn_n'nnGTC
Cuts at: 0 48 873
Size: 48 825
AleI CACnn'nnGTG
Cuts at: 0 431 873
Size: 431 442
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 140 172 873
Size: 140 32 701
Fragments arranged by size:
701 140 32
AlwNI CAG_nnn'CTG
Cuts at: 0 217 873
Size: 217 656
BanI G'GyrC_C
Cuts at: 0 644 873
Size: 644 229
BanII G_rGCy'C
Cuts at: 0 278 873
Size: 278 595
BbeI G_GCGC'C
Cuts at: 0 648 873
Size: 648 225
BfrBI ATG'CAT
Cuts at: 0 172 873
Size: 172 701
BglII A'GATC_T
Cuts at: 0 538 873
Size: 538 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 670 873
Size: 239 431 203
Fragments arranged by size:
431 239 203
BsaBI GATnn'nnATC
Cuts at: 0 537 873
Size: 537 336
BsaHI Gr'CG_yC
Cuts at: 0 645 873
Size: 645 228
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 781 838 873
Size: 781 57 35
Fragments arranged by size:
781 57 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 473 873
Size: 305 168 400
Fragments arranged by size:
400 305 168
Bsp1286I G_dGCh'C
Cuts at: 0 278 873
Size: 278 595
BspHI T'CATG_A
Cuts at: 0 849 873
Size: 849 24
BsrFI r'CCGG_y
Cuts at: 0 241 873
Size: 241 632
BsrGI T'GTAC_A
Cuts at: 0 177 873
Size: 177 696
BstAPI GCAn_nnn'nTGC
Cuts at: 0 208 873
Size: 208 665
BstYI r'GATC_y
Cuts at: 0 538 873
Size: 538 335
BtgI C'CryG_G
Cuts at: 0 426 873
Size: 426 447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 222 873
Size: 222 651
BtsI GCAGTG_nn'
Cuts at: 0 218 873
Size: 218 655
EaeI y'GGCC_r
Cuts at: 0 243 873
Size: 243 630
EarI CTCTTCn'nnn_
Cuts at: 0 701 797 873
Size: 701 96 76
Fragments arranged by size:
701 96 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 691 873
Size: 691 182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 487 670 797 873
Size: 239 248 183 127 76
Fragments arranged by size:
248 239 183 127 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 776 873
Size: 776 97
EcoO109I rG'GnC_Cy
Cuts at: 0 311 873
Size: 311 562
EcoRV GAT'ATC
Cuts at: 0 258 873
Size: 258 615
HaeII r_GCGC'y
Cuts at: 0 648 873
Size: 648 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 470 502 738 770 873
Size: 34 32 404 32 236 32 103
Fragments arranged by size:
404 236 103 34 32 32 32
HincII GTy'rAC
Cuts at: 0 108 557 873
Size: 108 449 316
Fragments arranged by size:
449 316 108
KasI G'GCGC_C
Cuts at: 0 644 873
Size: 644 229
MfeI C'AATT_G
Cuts at: 0 92 700 873
Size: 92 608 173
Fragments arranged by size:
608 173 92
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 873
Size: 126 747
MslI CAynn'nnrTG
Cuts at: 0 431 873
Size: 431 442
MspA1I CmG'CkG
Cuts at: 0 465 873
Size: 465 408
NaeI GCC'GGC
Cuts at: 0 243 873
Size: 243 630
NarI GG'CG_CC
Cuts at: 0 645 873
Size: 645 228
NgoMIV G'CCGG_C
Cuts at: 0 241 873
Size: 241 632
NsiI A_TGCA'T
Cuts at: 0 174 873
Size: 174 699
NspI r_CATG'y
Cuts at: 0 202 873
Size: 202 671
PflMI CCAn_nnn'nTGG
Cuts at: 0 298 873
Size: 298 575
PstI C_TGCA'G
Cuts at: 0 213 873
Size: 213 660
SfcI C'TryA_G
Cuts at: 0 209 390 761 862 873
Size: 209 181 371 101 11
Fragments arranged by size:
371 209 181 101 11
SfoI GGC'GCC
Cuts at: 0 646 873
Size: 646 227
TatI w'GTAC_w
Cuts at: 0 177 182 873
Size: 177 5 691
Fragments arranged by size:
691 177 5
Enzymes that do cut:
AcuI AhdI AleI AloI AlwNI BanI BanII BbeI
BfrBI BglII BpmI BsaBI BsaHI BseRI BsgI Bsp1286I
BspHI BsrFI BsrGI BstAPI BstYI BtgI BtgZI BtsI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV HaeII
Hin4I HincII KasI MfeI MmeI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PflMI PstI SfcI SfoI
TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoRI FalI
FseI FspI FspAI HindIII HpaI KpnI MluI MscI
NcoI NdeI NheI NotI NruI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI