Restriction map for
pFN21AE5307
(Linear) (Six-base) MAPSORT of: KIEE5307.seq Check: 5810 from: 1 to: 810
KIEE5307.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AccI GT'mk_AC
Cuts at: 0 364 537 638 810
Size: 364 173 101 172
Fragments arranged by size:
364 173 172 101
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 810
Size: 209 601
AleI CACnn'nnGTG
Cuts at: 0 721 810
Size: 721 89
AlwNI CAG_nnn'CTG
Cuts at: 0 116 810
Size: 116 694
ApoI r'AATT_y
Cuts at: 0 520 810
Size: 520 290
AvaI C'yCGr_G
Cuts at: 0 169 810
Size: 169 641
BamHI G'GATC_C
Cuts at: 0 591 810
Size: 591 219
BanI G'GyrC_C
Cuts at: 0 154 810
Size: 154 656
BanII G_rGCy'C
Cuts at: 0 671 810
Size: 671 139
BbeI G_GCGC'C
Cuts at: 0 158 810
Size: 158 652
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 810
Size: 134 676
BglI GCCn_nnn'nGGC
Cuts at: 0 139 810
Size: 139 671
BmrI ACTGGGnnnn_n'
Cuts at: 0 231 810
Size: 231 579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 581 810
Size: 138 443 229
Fragments arranged by size:
443 229 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 810
Size: 807 3
BsaHI Gr'CG_yC
Cuts at: 0 53 131 155 810
Size: 53 78 24 655
Fragments arranged by size:
655 78 53 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 346 376 739 769 810
Size: 346 30 363 30 41
Fragments arranged by size:
363 346 41 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 286 423 810
Size: 151 135 137 387
Fragments arranged by size:
387 151 137 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 810
Size: 251 559
BsiEI CG_ry'CG
Cuts at: 0 456 810
Size: 456 354
BsiHKAI G_wGCw'C
Cuts at: 0 149 810
Size: 149 661
BsmI GAATG_Cn'
Cuts at: 0 497 810
Size: 497 313
Bsp1286I G_dGCh'C
Cuts at: 0 149 671 810
Size: 149 522 139
Fragments arranged by size:
522 149 139
BsrFI r'CCGG_y
Cuts at: 0 372 810
Size: 372 438
BstYI r'GATC_y
Cuts at: 0 591 810
Size: 591 219
BtgI C'CryG_G
Cuts at: 0 150 810
Size: 150 660
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 90 810
Size: 90 720
EarI CTCTTCn'nnn_
Cuts at: 0 309 810
Size: 309 501
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 209 581 810
Size: 138 71 372 229
Fragments arranged by size:
372 229 138 71
EcoO109I rG'GnC_Cy
Cuts at: 0 193 810
Size: 193 617
HaeII r_GCGC'y
Cuts at: 0 117 158 810
Size: 117 41 652
Fragments arranged by size:
652 117 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 346 378 631 663 666 688
Size: 310 32 4 32 253 32 3 22
Cuts at: 688 698 720 810
Size: 10 22 90
Fragments arranged by size:
310 253 90 32 32 32 22 22
10 4 3
HincII GTy'rAC
Cuts at: 0 264 538 810
Size: 264 274 272
Fragments arranged by size:
274 272 264
HindIII A'AGCT_T
Cuts at: 0 767 810
Size: 767 43
HpaI GTT'AAC
Cuts at: 0 264 810
Size: 264 546
KasI G'GCGC_C
Cuts at: 0 154 810
Size: 154 656
MfeI C'AATT_G
Cuts at: 0 443 810
Size: 443 367
MslI CAynn'nnrTG
Cuts at: 0 721 810
Size: 721 89
MspA1I CmG'CkG
Cuts at: 0 41 152 601 810
Size: 41 111 449 209
Fragments arranged by size:
449 209 111 41
NaeI GCC'GGC
Cuts at: 0 374 810
Size: 374 436
NarI GG'CG_CC
Cuts at: 0 155 810
Size: 155 655
NgoMIV G'CCGG_C
Cuts at: 0 372 810
Size: 372 438
PstI C_TGCA'G
Cuts at: 0 654 810
Size: 654 156
PvuI CG_AT'CG
Cuts at: 0 456 810
Size: 456 354
SacII CC_GC'GG
Cuts at: 0 153 810
Size: 153 657
SalI G'TCGA_C
Cuts at: 0 536 810
Size: 536 274
SfcI C'TryA_G
Cuts at: 0 635 650 810
Size: 635 15 160
Fragments arranged by size:
635 160 15
SfoI GGC'GCC
Cuts at: 0 156 810
Size: 156 654
SmlI C'TyrA_G
Cuts at: 0 786 810
Size: 786 24
StyI C'CwwG_G
Cuts at: 0 719 810
Size: 719 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 729 810
Size: 729 81
TatI w'GTAC_w
Cuts at: 0 701 810
Size: 701 109
Enzymes that do cut:
AccI AcuI AleI AlwNI ApoI AvaI BamHI BanI
BanII BbeI BbsI BglI BmrI BpmI BpuEI BsaHI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrFI
BstYI BtgI BtgZI EarI Eco57MI EcoO109I HaeII Hin4I
HincII HindIII HpaI KasI MfeI MslI MspA1I NaeI
NarI NgoMIV PstI PvuI SacII SalI SfcI SfoI
SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaWI BseYI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI KpnI MluI MmeI MscI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI