Restriction map for
pFN21AE5306
(Linear) (Six-base) MAPSORT of: KIEE5306.seq Check: 1419 from: 1 to: 936
KIEE5306.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 175 936
Size: 175 761
Acc65I G'GTAC_C
Cuts at: 0 134 936
Size: 134 802
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 936
Size: 517 419
AleI CACnn'nnGTG
Cuts at: 0 539 936
Size: 539 397
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 557 936
Size: 557 379
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 592 624 936
Size: 592 32 312
Fragments arranged by size:
592 312 32
AlwNI CAG_nnn'CTG
Cuts at: 0 185 738 906 936
Size: 185 553 168 30
Fragments arranged by size:
553 185 168 30
ApaI G_GGCC'C
Cuts at: 0 381 799 836 892 936
Size: 381 418 37 56 44
Fragments arranged by size:
418 381 56 44 37
ApaLI G'TGCA_C
Cuts at: 0 532 936
Size: 532 404
AvaI C'yCGr_G
Cuts at: 0 145 588 784 936
Size: 145 443 196 152
Fragments arranged by size:
443 196 152 145
BanI G'GyrC_C
Cuts at: 0 134 936
Size: 134 802
BanII G_rGCy'C
Cuts at: 0 381 403 718 799 836 892 936
Size: 381 22 315 81 37 56 44
Fragments arranged by size:
381 315 81 56 44 37 22
BglI GCCn_nnn'nGGC
Cuts at: 0 763 840 936
Size: 763 77 96
Fragments arranged by size:
763 96 77
BglII A'GATC_T
Cuts at: 0 438 936
Size: 438 498
Bme1580I G_kGCm'C
Cuts at: 0 317 381 536 546 799 836 892 936
Size: 317 64 155 10 253 37 56 44
Fragments arranged by size:
317 253 155 64 56 44 37 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 81 936
Size: 81 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 936
Size: 732 204
BsaWI w'CCGG_w
Cuts at: 0 38 927 936
Size: 38 889 9
Fragments arranged by size:
889 38 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 10 40 936
Size: 10 30 896
Fragments arranged by size:
896 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 836 936
Size: 836 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 657 772 936
Size: 607 50 115 164
Fragments arranged by size:
607 164 115 50
BsiEI CG_ry'CG
Cuts at: 0 607 840 936
Size: 607 233 96
Fragments arranged by size:
607 233 96
BsiHKAI G_wGCw'C
Cuts at: 0 536 936
Size: 536 400
Bsp1286I G_dGCh'C
Cuts at: 0 317 381 403 536 546 718 799 836
Size: 317 64 22 133 10 172 81 37
Cuts at: 836 892 936
Size: 56 44
Fragments arranged by size:
317 172 133 81 64 56 44 37
22 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 175 644 936
Size: 175 469 292
Fragments arranged by size:
469 292 175
BsrBI CCG'CTC
Cuts at: 0 276 504 936
Size: 276 228 432
Fragments arranged by size:
432 276 228
BsrDI GCAATG_nn'
Cuts at: 0 301 936
Size: 301 635
BsrFI r'CCGG_y
Cuts at: 0 247 602 839 936
Size: 247 355 237 97
Fragments arranged by size:
355 247 237 97
BssSI C'ACGA_G
Cuts at: 0 442 748 936
Size: 442 306 188
Fragments arranged by size:
442 306 188
BstEII G'GTnAC_C
Cuts at: 0 333 936
Size: 333 603
BstXI CCAn_nnnn'nTGG
Cuts at: 0 352 936
Size: 352 584
BstYI r'GATC_y
Cuts at: 0 438 936
Size: 438 498
BtgI C'CryG_G
Cuts at: 0 557 647 936
Size: 557 90 289
Fragments arranged by size:
557 289 90
BtsI GCAGTG_nn'
Cuts at: 0 534 546 724 907 936
Size: 534 12 178 183 29
Fragments arranged by size:
534 183 178 29 12
DraIII CAC_nnn'GTG
Cuts at: 0 389 558 659 936
Size: 389 169 101 277
Fragments arranged by size:
389 277 169 101
EaeI y'GGCC_r
Cuts at: 0 152 604 837 936
Size: 152 452 233 99
Fragments arranged by size:
452 233 152 99
EagI C'GGCC_G
Cuts at: 0 604 837 936
Size: 604 233 99
Fragments arranged by size:
604 233 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 732 936
Size: 517 215 204
Fragments arranged by size:
517 215 204
EcoNI CCTnn'n_nnAGG
Cuts at: 0 235 936
Size: 235 701
EcoO109I rG'GnC_Cy
Cuts at: 0 795 796 888 889 936
Size: 795 1 92 1 47
Fragments arranged by size:
795 92 47 1 1
FseI GG_CCGG'CC
Cuts at: 0 843 936
Size: 843 93
FspI TGC'GCA
Cuts at: 0 640 936
Size: 640 296
HaeII r_GCGC'y
Cuts at: 0 220 936
Size: 220 716
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 466 498 936
Size: 10 32 424 32 438
Fragments arranged by size:
438 424 32 32 10
HincII GTy'rAC
Cuts at: 0 63 936
Size: 63 873
KpnI G_GTAC'C
Cuts at: 0 138 936
Size: 138 798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 936
Size: 890 46
MslI CAynn'nnrTG
Cuts at: 0 539 936
Size: 539 397
MspA1I CmG'CkG
Cuts at: 0 129 195 212 525 885 936
Size: 129 66 17 313 360 51
Fragments arranged by size:
360 313 129 66 51 17
NaeI GCC'GGC
Cuts at: 0 249 841 936
Size: 249 592 95
Fragments arranged by size:
592 249 95
NgoMIV G'CCGG_C
Cuts at: 0 247 839 936
Size: 247 592 97
Fragments arranged by size:
592 247 97
PasI CC'CwG_GG
Cuts at: 0 546 936
Size: 546 390
PflMI CCAn_nnn'nTGG
Cuts at: 0 195 558 936
Size: 195 363 378
Fragments arranged by size:
378 363 195
PfoI T'CCnGG_A
Cuts at: 0 320 936
Size: 320 616
PspOMI G'GGCC_C
Cuts at: 0 377 795 832 888 936
Size: 377 418 37 56 48
Fragments arranged by size:
418 377 56 48 37
PstI C_TGCA'G
Cuts at: 0 122 541 734 902 936
Size: 122 419 193 168 34
Fragments arranged by size:
419 193 168 122 34
PvuII CAG'CTG
Cuts at: 0 129 195 525 936
Size: 129 66 330 411
Fragments arranged by size:
411 330 129 66
SfcI C'TryA_G
Cuts at: 0 118 537 730 898 936
Size: 118 419 193 168 38
Fragments arranged by size:
419 193 168 118 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 763 840 936
Size: 763 77 96
Fragments arranged by size:
763 96 77
StuI AGG'CCT
Cuts at: 0 757 936
Size: 757 179
StyI C'CwwG_G
Cuts at: 0 287 936
Size: 287 649
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 643 675 936
Size: 643 32 261
Fragments arranged by size:
643 261 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 294 936
Size: 294 642
Enzymes that do cut:
AarI Acc65I AcuI AleI AlfI AloI AlwNI ApaI
ApaLI AvaI BanI BanII BglI BglII Bme1580I BmrI
BpmI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI
BtgI BtsI DraIII EaeI EagI Eco57MI EcoNI EcoO109I
FseI FspI HaeII Hin4I HincII KpnI MmeI MslI
MspA1I NaeI NgoMIV PasI PflMI PfoI PspOMI PstI
PvuII SfcI SfiI StuI StyI TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BseYI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EarI EciI EcoICRI EcoRI EcoRV FalI FspAI
HindIII HpaI KasI MfeI MluI MscI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I
XbaI XhoI XmaI XmnI ZraI