(Linear) (Six-base) MAPSORT of: KIEE5305.seq  Check: 4097  from: 1  to: 855



KIEE5305.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:54  ..

AccI GT'mk_AC

Cuts at:      0     250     855 
   Size:        250     605
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312     809     855 
   Size:        312     497      46

  Fragments arranged by size: 

                 497     312      46
 
AflII C'TTAA_G

Cuts at:      0      66     855 
   Size:         66     789
 
AvaI C'yCGr_G

Cuts at:      0     833     855 
   Size:        833      22
 
BamHI G'GATC_C

Cuts at:      0     194     855 
   Size:        194     661
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     797     855 
   Size:        797      58
 
BglII A'GATC_T

Cuts at:      0     590     825     855 
   Size:        590     235      30

  Fragments arranged by size: 

                 590     235      30
 
Bme1580I G_kGCm'C

Cuts at:      0     209     855 
   Size:        209     646
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     855 
   Size:        628     227
 
BsaAI yAC'GTr

Cuts at:      0      79     855 
   Size:         79     776
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     279     538     568     855 
   Size:        279     259      30     287

  Fragments arranged by size: 

                 287     279     259      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     209     855 
   Size:        209     646
 
BspHI T'CATG_A

Cuts at:      0     356     855 
   Size:        356     499
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     434     502     855 
   Size:        434      68     353

  Fragments arranged by size: 

                 434     353      68
 
BstYI r'GATC_y

Cuts at:      0      55     194     590     825     855 
   Size:         55     139     396     235      30

  Fragments arranged by size: 

                 396     235     139      55      30
 
BstZ17I GTA'TAC

Cuts at:      0     251     855 
   Size:        251     604
 
BtgI C'CryG_G

Cuts at:      0     847     855 
   Size:        847       8
 
DraIII CAC_nnn'GTG

Cuts at:      0     262     855 
   Size:        262     593
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312     809     855 
   Size:        312     497      46

  Fragments arranged by size: 

                 497     312      46
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     342     748     855 
   Size:        342     406     107

  Fragments arranged by size: 

                 406     342     107
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     810     842     855 
   Size:        810      32      13

  Fragments arranged by size: 

                 810      32      13
 
MslI CAynn'nnrTG

Cuts at:      0     500     855 
   Size:        500     355
 
NcoI C'CATG_G

Cuts at:      0     847     855 
   Size:        847       8
 
PasI CC'CwG_GG

Cuts at:      0     227     855 
   Size:        227     628
 
SmlI C'TyrA_G

Cuts at:      0      66     643     833     855 
   Size:         66     577     190      22

  Fragments arranged by size: 

                 577     190      66      22
 
SspI AAT'ATT

Cuts at:      0     116     445     855 
   Size:        116     329     410

  Fragments arranged by size: 

                 410     329     116
 
StyI C'CwwG_G

Cuts at:      0     176     335     341     847     855 
   Size:        176     159       6     506       8

  Fragments arranged by size: 

                 506     176     159       8       6
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     175     207     855 
   Size:        175      32     648

  Fragments arranged by size: 

                 648     175      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     375     855 
   Size:        375     480
 
XhoI C'TCGA_G

Cuts at:      0     833     855 
   Size:        833      22
 
XmnI GAAnn'nnTTC

Cuts at:      0     532     855 
   Size:        532     323
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AvaI    BamHI     BbsI    BglII Bme1580I
    BpuEI    BsaAI    BseRI Bsp1286I    BspHI    BstXI    BstYI  BstZ17I
     BtgI   DraIII  Eco57MI     FalI    Hin4I     MslI     NcoI     PasI
     SmlI     SspI     StyI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XmaI     ZraI