Restriction map for
pFN21AE5304
(Linear) (Six-base) MAPSORT of: KIEE5304.seq Check: 5722 from: 1 to: 798
KIEE5304.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AatII G_ACGT'C
Cuts at: 0 383 576 798
Size: 383 193 222
Fragments arranged by size:
383 222 193
AflII C'TTAA_G
Cuts at: 0 286 798
Size: 286 512
AhdI GACnn_n'nnGTC
Cuts at: 0 400 577 798
Size: 400 177 221
Fragments arranged by size:
400 221 177
AleI CACnn'nnGTG
Cuts at: 0 436 616 710 798
Size: 436 180 94 88
Fragments arranged by size:
436 180 94 88
AlwNI CAG_nnn'CTG
Cuts at: 0 258 339 798
Size: 258 81 459
Fragments arranged by size:
459 258 81
ApoI r'AATT_y
Cuts at: 0 111 798
Size: 111 687
AvaI C'yCGr_G
Cuts at: 0 347 798
Size: 347 451
BamHI G'GATC_C
Cuts at: 0 483 798
Size: 483 315
BbsI GAAGACnn'nnnn_
Cuts at: 0 495 595 635 798
Size: 495 100 40 163
Fragments arranged by size:
495 163 100 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 585 619 798
Size: 585 34 179
Fragments arranged by size:
585 179 34
BlpI GC'TnA_GC
Cuts at: 0 455 647 798
Size: 455 192 151
Fragments arranged by size:
455 192 151
BmgBI CAC'GTC
Cuts at: 0 480 798
Size: 480 318
BmrI ACTGGGnnnn_n'
Cuts at: 0 577 717 798
Size: 577 140 81
Fragments arranged by size:
577 140 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 223 798
Size: 223 575
BsaI GGTCTCn'nnnn_
Cuts at: 0 140 387 798
Size: 140 247 411
Fragments arranged by size:
411 247 140
BsaHI Gr'CG_yC
Cuts at: 0 380 573 798
Size: 380 193 225
Fragments arranged by size:
380 225 193
BseYI C'CCAG_C
Cuts at: 0 45 798
Size: 45 753
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 798
Size: 579 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 601 798
Size: 601 197
BsrDI GCAATG_nn'
Cuts at: 0 723 798
Size: 723 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 438 495 792 798
Size: 438 57 297 6
Fragments arranged by size:
438 297 57 6
BstYI r'GATC_y
Cuts at: 0 483 695 798
Size: 483 212 103
Fragments arranged by size:
483 212 103
DraIII CAC_nnn'GTG
Cuts at: 0 480 798
Size: 480 318
DrdI GACnn_nn'nnGTC
Cuts at: 0 572 798
Size: 572 226
EarI CTCTTCn'nnn_
Cuts at: 0 385 798
Size: 385 413
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 688 798
Size: 688 110
EcoO109I rG'GnC_Cy
Cuts at: 0 255 534 727 798
Size: 255 279 193 71
Fragments arranged by size:
279 255 193 71
HaeII r_GCGC'y
Cuts at: 0 641 798
Size: 641 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 116 148 386 418 563 595 687 719
Size: 116 32 238 32 145 32 92 32
Cuts at: 719 798
Size: 79
Fragments arranged by size:
238 145 116 92 79 32 32 32
32
HincII GTy'rAC
Cuts at: 0 420 798
Size: 420 378
HpaI GTT'AAC
Cuts at: 0 420 798
Size: 420 378
MslI CAynn'nnrTG
Cuts at: 0 436 616 710 798
Size: 436 180 94 88
Fragments arranged by size:
436 180 94 88
MspA1I CmG'CkG
Cuts at: 0 49 192 282 459 798
Size: 49 143 90 177 339
Fragments arranged by size:
339 177 143 90 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 258 798
Size: 258 540
PpuMI rG'GwC_Cy
Cuts at: 0 534 727 798
Size: 534 193 71
Fragments arranged by size:
534 193 71
PvuII CAG'CTG
Cuts at: 0 49 282 459 798
Size: 49 233 177 339
Fragments arranged by size:
339 233 177 49
SanDI GG'GwC_CC
Cuts at: 0 534 798
Size: 534 264
SmaI CCC'GGG
Cuts at: 0 349 798
Size: 349 449
SmlI C'TyrA_G
Cuts at: 0 286 798
Size: 286 512
SrfI GCCC'GGGC
Cuts at: 0 349 798
Size: 349 449
StuI AGG'CCT
Cuts at: 0 105 798
Size: 105 693
StyI C'CwwG_G
Cuts at: 0 20 434 798
Size: 20 414 364
Fragments arranged by size:
414 364 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 798
Size: 265 533
TatI w'GTAC_w
Cuts at: 0 302 798
Size: 302 496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 798
Size: 17 32 749
Fragments arranged by size:
749 32 17
XmaI C'CCGG_G
Cuts at: 0 347 798
Size: 347 451
ZraI GAC'GTC
Cuts at: 0 381 574 798
Size: 381 193 224
Fragments arranged by size:
381 224 193
Enzymes that do cut:
AatII AflII AhdI AleI AlwNI ApoI AvaI BamHI
BbsI BcgI BlpI BmgBI BmrI BplI BsaI BsaHI
BseYI BsgI BspMI BsrDI BstXI BstYI DraIII DrdI
EarI EciI EcoO109I HaeII Hin4I HincII HpaI MslI
MspA1I PflMI PpuMI PvuII SanDI SmaI SmlI SrfI
StuI StyI TaqII TatI TstI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BanII BbeI BbvCI BciVI BclI BfrBI
BglI BglII Bme1580I BmtI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI EaeI EagI EcoICRI Eco57MI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
Tth111I XbaI XcmI XhoI XmnI