Restriction map for
pFN21AE5303
(Linear) (Six-base) MAPSORT of: KIEE5303.seq Check: 9327 from: 1 to: 789
KIEE5303.orf
With 182 enzymes: SgfI *
December 5, 2014 13:00 ..
AclI AA'CG_TT
Cuts at: 0 201 789
Size: 201 588
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 789
Size: 767 22
AlwNI CAG_nnn'CTG
Cuts at: 0 454 789
Size: 454 335
ApaI G_GGCC'C
Cuts at: 0 13 789
Size: 13 776
ApoI r'AATT_y
Cuts at: 0 520 789
Size: 520 269
AvaI C'yCGr_G
Cuts at: 0 5 789
Size: 5 784
BanII G_rGCy'C
Cuts at: 0 13 789
Size: 13 776
BbsI GAAGACnn'nnnn_
Cuts at: 0 115 129 286 789
Size: 115 14 157 503
Fragments arranged by size:
503 157 115 14
BbvCI CC'TCA_GC
Cuts at: 0 112 789
Size: 112 677
BclI T'GATC_A
Cuts at: 0 581 789
Size: 581 208
Bme1580I G_kGCm'C
Cuts at: 0 13 789
Size: 13 776
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 580 789
Size: 580 209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 789
Size: 191 598
Bpu10I CC'TnA_GC
Cuts at: 0 112 789
Size: 112 677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 789
Size: 139 650
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 164 194 789
Size: 164 30 595
Fragments arranged by size:
595 164 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 789
Size: 499 290
BsiEI CG_ry'CG
Cuts at: 0 570 789
Size: 570 219
BsmI GAATG_Cn'
Cuts at: 0 728 789
Size: 728 61
Bsp1286I G_dGCh'C
Cuts at: 0 13 789
Size: 13 776
BspHI T'CATG_A
Cuts at: 0 423 789
Size: 423 366
BsrDI GCAATG_nn'
Cuts at: 0 693 789
Size: 693 96
BssSI C'ACGA_G
Cuts at: 0 657 789
Size: 657 132
BstAPI GCAn_nnn'nTGC
Cuts at: 0 54 789
Size: 54 735
BstYI r'GATC_y
Cuts at: 0 450 789
Size: 450 339
Bsu36I CC'TnA_GG
Cuts at: 0 142 789
Size: 142 647
BtsI GCAGTG_nn'
Cuts at: 0 789
Size: 789
DraIII CAC_nnn'GTG
Cuts at: 0 417 789
Size: 417 372
EaeI y'GGCC_r
Cuts at: 0 767 789
Size: 767 22
EarI CTCTTCn'nnn_
Cuts at: 0 344 743 789
Size: 344 399 46
Fragments arranged by size:
399 344 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 286 789
Size: 286 503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 767 789
Size: 191 576 22
Fragments arranged by size:
576 191 22
EcoO109I rG'GnC_Cy
Cuts at: 0 9 10 100 789
Size: 9 1 90 689
Fragments arranged by size:
689 90 9 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 39 789
Size: 39 750
HaeII r_GCGC'y
Cuts at: 0 43 789
Size: 43 746
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 221 253 789
Size: 105 32 84 32 536
Fragments arranged by size:
536 105 84 32 32
HincII GTy'rAC
Cuts at: 0 167 789
Size: 167 622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 789
Size: 692 97
MscI TGG'CCA
Cuts at: 0 769 789
Size: 769 20
MslI CAynn'nnrTG
Cuts at: 0 343 463 789
Size: 343 120 326
Fragments arranged by size:
343 326 120
NspI r_CATG'y
Cuts at: 0 628 789
Size: 628 161
PasI CC'CwG_GG
Cuts at: 0 716 789
Size: 716 73
PpuMI rG'GwC_Cy
Cuts at: 0 100 789
Size: 100 689
PspOMI G'GGCC_C
Cuts at: 0 9 789
Size: 9 780
SexAI A'CCwGG_T
Cuts at: 0 413 789
Size: 413 376
SmaI CCC'GGG
Cuts at: 0 7 789
Size: 7 782
SmlI C'TyrA_G
Cuts at: 0 154 789
Size: 154 635
StyI C'CwwG_G
Cuts at: 0 314 700 789
Size: 314 386 89
Fragments arranged by size:
386 314 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 556 789
Size: 556 233
TatI w'GTAC_w
Cuts at: 0 359 789
Size: 359 430
XmaI C'CCGG_G
Cuts at: 0 5 789
Size: 5 784
XmnI GAAnn'nnTTC
Cuts at: 0 122 789
Size: 122 667
Enzymes that do cut:
AclI AcuI AlwNI ApaI ApoI AvaI BanII BbsI
BbvCI BclI Bme1580I BplI BpmI Bpu10I BpuEI BsaXI
BsgI BsiEI BsmI Bsp1286I BspHI BsrDI BssSI BstAPI
BstYI Bsu36I BtsI DraIII EaeI EarI EciI Eco57MI
EcoO109I FalI HaeII Hin4I HincII MmeI MscI MslI
NspI PasI PpuMI PspOMI SexAI SmaI SmlI StyI
TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiHKAI BsiWI BsmBI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI ZraI