Restriction map for
pFN21AE5301
(Linear) (Six-base) MAPSORT of: KIEE5301.seq Check: 9490 from: 1 to: 780
KIEE5301.orf
With 182 enzymes: SgfI *
December 5, 2014 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 313 780
Size: 313 467
ApaI G_GGCC'C
Cuts at: 0 594 780
Size: 594 186
ApaLI G'TGCA_C
Cuts at: 0 258 780
Size: 258 522
ApoI r'AATT_y
Cuts at: 0 352 780
Size: 352 428
AvaI C'yCGr_G
Cuts at: 0 97 780
Size: 97 683
BanI G'GyrC_C
Cuts at: 0 145 172 464 767 780
Size: 145 27 292 303 13
Fragments arranged by size:
303 292 145 27 13
BanII G_rGCy'C
Cuts at: 0 194 594 780
Size: 194 400 186
Fragments arranged by size:
400 194 186
BbeI G_GCGC'C
Cuts at: 0 149 780
Size: 149 631
BglI GCCn_nnn'nGGC
Cuts at: 0 154 542 735 780
Size: 154 388 193 45
Fragments arranged by size:
388 193 154 45
Bme1580I G_kGCm'C
Cuts at: 0 175 262 290 594 770 780
Size: 175 87 28 304 176 10
Fragments arranged by size:
304 176 175 87 28 10
BmgBI CAC'GTC
Cuts at: 0 285 780
Size: 285 495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 780
Size: 586 194
BsaHI Gr'CG_yC
Cuts at: 0 94 146 780
Size: 94 52 634
Fragments arranged by size:
634 94 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 634 664 780
Size: 634 30 116
Fragments arranged by size:
634 116 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 780
Size: 43 737
BseYI C'CCAG_C
Cuts at: 0 438 780
Size: 438 342
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 518 780
Size: 324 194 262
Fragments arranged by size:
324 262 194
BsiHKAI G_wGCw'C
Cuts at: 0 194 262 488 780
Size: 194 68 226 292
Fragments arranged by size:
292 226 194 68
BsmI GAATG_Cn'
Cuts at: 0 378 579 780
Size: 378 201 201
Fragments arranged by size:
378 201 201
Bsp1286I G_dGCh'C
Cuts at: 0 175 194 262 290 488 594 770 780
Size: 175 19 68 28 198 106 176 10
Fragments arranged by size:
198 176 175 106 68 28 19 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 71 313 780
Size: 71 242 467
Fragments arranged by size:
467 242 71
BsrFI r'CCGG_y
Cuts at: 0 35 56 679 703 780
Size: 35 21 623 24 77
Fragments arranged by size:
623 77 35 24 21
BtgI C'CryG_G
Cuts at: 0 50 140 167 290 314 362 780
Size: 50 90 27 123 24 48 418
Fragments arranged by size:
418 123 90 50 48 27 24
BtsI GCAGTG_nn'
Cuts at: 0 440 724 780
Size: 440 284 56
Fragments arranged by size:
440 284 56
DraI TTT'AAA
Cuts at: 0 351 780
Size: 351 429
EaeI y'GGCC_r
Cuts at: 0 134 780
Size: 134 646
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 295 780
Size: 295 485
EcoICRI GAG'CTC
Cuts at: 0 192 780
Size: 192 588
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 780
Size: 586 194
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 780
Size: 25 755
EcoO109I rG'GnC_Cy
Cuts at: 0 590 591 746 780
Size: 590 1 155 34
Fragments arranged by size:
590 155 34 1
FseI GG_CCGG'CC
Cuts at: 0 683 780
Size: 683 97
HaeII r_GCGC'y
Cuts at: 0 127 149 221 780
Size: 127 22 72 559
Fragments arranged by size:
559 127 72 22
KasI G'GCGC_C
Cuts at: 0 145 780
Size: 145 635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 498 780
Size: 35 463 282
Fragments arranged by size:
463 282 35
MslI CAynn'nnrTG
Cuts at: 0 481 780
Size: 481 299
MspA1I CmG'CkG
Cuts at: 0 52 316 421 780
Size: 52 264 105 359
Fragments arranged by size:
359 264 105 52
NaeI GCC'GGC
Cuts at: 0 58 681 705 780
Size: 58 623 24 75
Fragments arranged by size:
623 75 58 24
NarI GG'CG_CC
Cuts at: 0 146 780
Size: 146 634
NgoMIV G'CCGG_C
Cuts at: 0 56 679 703 780
Size: 56 623 24 77
Fragments arranged by size:
623 77 56 24
PasI CC'CwG_GG
Cuts at: 0 660 740 780
Size: 660 80 40
Fragments arranged by size:
660 80 40
PpuMI rG'GwC_Cy
Cuts at: 0 746 780
Size: 746 34
PspOMI G'GGCC_C
Cuts at: 0 590 780
Size: 590 190
PstI C_TGCA'G
Cuts at: 0 602 780
Size: 602 178
PvuII CAG'CTG
Cuts at: 0 421 780
Size: 421 359
SacI G_AGCT'C
Cuts at: 0 194 780
Size: 194 586
SacII CC_GC'GG
Cuts at: 0 53 317 780
Size: 53 264 463
Fragments arranged by size:
463 264 53
SanDI GG'GwC_CC
Cuts at: 0 746 780
Size: 746 34
ScaI AGT'ACT
Cuts at: 0 649 780
Size: 649 131
SfcI C'TryA_G
Cuts at: 0 598 780
Size: 598 182
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 542 780
Size: 542 238
SfoI GGC'GCC
Cuts at: 0 147 780
Size: 147 633
StuI AGG'CCT
Cuts at: 0 545 780
Size: 545 235
StyI C'CwwG_G
Cuts at: 0 458 780
Size: 458 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 174 780
Size: 174 606
TatI w'GTAC_w
Cuts at: 0 647 780
Size: 647 133
Enzymes that do cut:
AarI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BglI Bme1580I BmgBI BpmI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BtgI BtsI
DraI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI
HaeII KasI MmeI MslI MspA1I NaeI NarI NgoMIV
PasI PpuMI PspOMI PstI PvuII SacI SacII SanDI
ScaI SfcI SfiI SfoI StuI StyI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EarI EcoRI EcoRV FalI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MscI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI
SbfI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI