Restriction map for
pFN21AE5293
(Linear) (Six-base) MAPSORT of: KIEE5293.seq Check: 6954 from: 1 to: 534
KIEE5293.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AatII G_ACGT'C
Cuts at: 0 419 534
Size: 419 115
AleI CACnn'nnGTG
Cuts at: 0 354 534
Size: 354 180
AlwNI CAG_nnn'CTG
Cuts at: 0 76 114 198 231 258 534
Size: 76 38 84 33 27 276
Fragments arranged by size:
276 84 76 38 33 27
ApaI G_GGCC'C
Cuts at: 0 307 534
Size: 307 227
AvaI C'yCGr_G
Cuts at: 0 54 393 534
Size: 54 339 141
Fragments arranged by size:
339 141 54
BanII G_rGCy'C
Cuts at: 0 307 534
Size: 307 227
BbvCI CC'TCA_GC
Cuts at: 0 199 534
Size: 199 335
Bme1580I G_kGCm'C
Cuts at: 0 307 393 534
Size: 307 86 141
Fragments arranged by size:
307 141 86
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 231 534
Size: 231 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 268 534
Size: 253 15 266
Fragments arranged by size:
266 253 15
Bpu10I CC'TnA_GC
Cuts at: 0 199 534
Size: 199 335
BsaAI yAC'GTr
Cuts at: 0 141 534
Size: 141 393
BsaHI Gr'CG_yC
Cuts at: 0 36 416 534
Size: 36 380 118
Fragments arranged by size:
380 118 36
BseYI C'CCAG_C
Cuts at: 0 107 165 271 534
Size: 107 58 106 263
Fragments arranged by size:
263 107 106 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 534
Size: 313 221
BsmBI CGTCTCn'nnnn_
Cuts at: 0 423 534
Size: 423 111
Bsp1286I G_dGCh'C
Cuts at: 0 307 393 534
Size: 307 86 141
Fragments arranged by size:
307 141 86
BsrBI CCG'CTC
Cuts at: 0 149 534
Size: 149 385
BssHII G'CGCG_C
Cuts at: 0 211 263 534
Size: 211 52 271
Fragments arranged by size:
271 211 52
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 534
Size: 336 198
BtgI C'CryG_G
Cuts at: 0 7 504 534
Size: 7 497 30
Fragments arranged by size:
497 30 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 438 534
Size: 438 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 268 534
Size: 253 15 266
Fragments arranged by size:
266 253 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 534
Size: 337 197
EcoO109I rG'GnC_Cy
Cuts at: 0 303 304 342 409 534
Size: 303 1 38 67 125
Fragments arranged by size:
303 125 67 38 1
HaeII r_GCGC'y
Cuts at: 0 166 534
Size: 166 368
MslI CAynn'nnrTG
Cuts at: 0 352 354 534
Size: 352 2 180
Fragments arranged by size:
352 180 2
MspA1I CmG'CkG
Cuts at: 0 506 534
Size: 506 28
PasI CC'CwG_GG
Cuts at: 0 76 92 534
Size: 76 16 442
Fragments arranged by size:
442 76 16
PmlI CAC'GTG
Cuts at: 0 141 534
Size: 141 393
PpuMI rG'GwC_Cy
Cuts at: 0 342 534
Size: 342 192
PspOMI G'GGCC_C
Cuts at: 0 303 534
Size: 303 231
PstI C_TGCA'G
Cuts at: 0 319 534
Size: 319 215
SacII CC_GC'GG
Cuts at: 0 507 534
Size: 507 27
SfcI C'TryA_G
Cuts at: 0 315 534
Size: 315 219
SmaI CCC'GGG
Cuts at: 0 395 534
Size: 395 139
StyI C'CwwG_G
Cuts at: 0 218 534
Size: 218 316
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 279 443 534
Size: 279 164 91
Fragments arranged by size:
279 164 91
XmaI C'CCGG_G
Cuts at: 0 393 534
Size: 393 141
ZraI GAC'GTC
Cuts at: 0 417 534
Size: 417 117
Enzymes that do cut:
AatII AleI AlwNI ApaI AvaI BanII BbvCI Bme1580I
BplI BpmI Bpu10I BsaAI BsaHI BseYI BsgI BsmBI
Bsp1286I BsrBI BssHII BstXI BtgI BtgZI Eco57MI EcoNI
EcoO109I HaeII MslI MspA1I PasI PmlI PpuMI PspOMI
PstI SacII SfcI SmaI StyI TaqII XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiEI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmnI