(Linear) (Six-base) MAPSORT of: KIEE5282.seq  Check: 780  from: 1  to: 756



KIEE5282.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:54  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     756 
   Size:        298     458
 
ApoI r'AATT_y

Cuts at:      0     230     571     756 
   Size:        230     341     185

  Fragments arranged by size: 

                 341     230     185
 
BanII G_rGCy'C

Cuts at:      0     203     756 
   Size:        203     553
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     168     756 
   Size:        168     588
 
BglI GCCn_nnn'nGGC

Cuts at:      0     160     756 
   Size:        160     596
 
BmtI G_CTAG'C

Cuts at:      0     737     756 
   Size:        737      19
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     512     756 
   Size:        512     244
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52     163     756 
   Size:         52     111     593

  Fragments arranged by size: 

                 593     111      52
 
BseYI C'CCAG_C

Cuts at:      0     240     756 
   Size:        240     516
 
BsiHKAI G_wGCw'C

Cuts at:      0     387     756 
   Size:        387     369
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     387     756 
   Size:        203     184     369

  Fragments arranged by size: 

                 369     203     184
 
BsrFI r'CCGG_y

Cuts at:      0     159     756 
   Size:        159     597
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     667     756 
   Size:        667      89
 
BstEII G'GTnAC_C

Cuts at:      0      54     756 
   Size:         54     702
 
ClaI AT'CG_AT

Cuts at:      0     750     756 
   Size:        750       6
 
EaeI y'GGCC_r

Cuts at:      0      69     756 
   Size:         69     687
 
EarI CTCTTCn'nnn_

Cuts at:      0     160     478     756 
   Size:        160     318     278

  Fragments arranged by size: 

                 318     278     160
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     756 
   Size:        298     458
 
HaeII r_GCGC'y

Cuts at:      0      29     756 
   Size:         29     727
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     273     305     511     543     756 
   Size:        273      32     206      32     213

  Fragments arranged by size: 

                 273     213     206      32      32
 
HindIII A'AGCT_T

Cuts at:      0     413     756 
   Size:        413     343
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     443     756 
   Size:        443     313
 
MspA1I CmG'CkG

Cuts at:      0     102     400     756 
   Size:        102     298     356

  Fragments arranged by size: 

                 356     298     102
 
NaeI GCC'GGC

Cuts at:      0     161     756 
   Size:        161     595
 
NgoMIV G'CCGG_C

Cuts at:      0     159     756 
   Size:        159     597
 
NheI G'CTAG_C

Cuts at:      0     733     756 
   Size:        733      23
 
PvuII CAG'CTG

Cuts at:      0     102     400     756 
   Size:        102     298     356

  Fragments arranged by size: 

                 356     298     102
 
SspI AAT'ATT

Cuts at:      0     720     756 
   Size:        720      36
 
StyI C'CwwG_G

Cuts at:      0     371     633     704     756 
   Size:        371     262      71      52

  Fragments arranged by size: 

                 371     262      71      52
 

 Enzymes that do cut:

     AcuI     ApoI    BanII     BbsI     BglI     BmtI     BsaI    BseRI
    BseYI  BsiHKAI Bsp1286I    BsrFI   BstAPI   BstEII     ClaI     EaeI
     EarI  Eco57MI    HaeII    Hin4I  HindIII     MmeI   MspA1I     NaeI
   NgoMIV     NheI    PvuII     SspI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NarI     NcoI     NdeI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI