Restriction map for
pFN21AE5281
(Linear) (Six-base) MAPSORT of: KIEE5281.seq Check: 4513 from: 1 to: 753
KIEE5281.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 324 753
Size: 324 429
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 297 753
Size: 129 168 456
Fragments arranged by size:
456 168 129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 674 706 753
Size: 674 32 47
Fragments arranged by size:
674 47 32
AlwNI CAG_nnn'CTG
Cuts at: 0 648 753
Size: 648 105
ApaI G_GGCC'C
Cuts at: 0 105 753
Size: 105 648
AvaI C'yCGr_G
Cuts at: 0 98 602 753
Size: 98 504 151
Fragments arranged by size:
504 151 98
BanII G_rGCy'C
Cuts at: 0 105 125 165 739 753
Size: 105 20 40 574 14
Fragments arranged by size:
574 105 40 20 14
BbvCI CC'TCA_GC
Cuts at: 0 126 350 753
Size: 126 224 403
Fragments arranged by size:
403 224 126
BglI GCCn_nnn'nGGC
Cuts at: 0 94 734 753
Size: 94 640 19
Fragments arranged by size:
640 94 19
Bme1580I G_kGCm'C
Cuts at: 0 105 753
Size: 105 648
BmgBI CAC'GTC
Cuts at: 0 32 753
Size: 32 721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 728 753
Size: 133 595 25
Fragments arranged by size:
595 133 25
Bpu10I CC'TnA_GC
Cuts at: 0 55 126 193 272 350 753
Size: 55 71 67 79 78 403
Fragments arranged by size:
403 79 78 71 67 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 674 704 753
Size: 674 30 49
Fragments arranged by size:
674 49 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 753
Size: 309 444
Bsp1286I G_dGCh'C
Cuts at: 0 105 125 165 739 753
Size: 105 20 40 574 14
Fragments arranged by size:
574 105 40 20 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 324 753
Size: 324 429
BsrFI r'CCGG_y
Cuts at: 0 8 753
Size: 8 745
Bsu36I CC'TnA_GG
Cuts at: 0 72 361 753
Size: 72 289 392
Fragments arranged by size:
392 289 72
BtgI C'CryG_G
Cuts at: 0 105 198 656 753
Size: 105 93 458 97
Fragments arranged by size:
458 105 97 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 273 753
Size: 273 480
BtsI GCAGTG_nn'
Cuts at: 0 401 753
Size: 401 352
DrdI GACnn_nn'nnGTC
Cuts at: 0 658 753
Size: 658 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 52 753
Size: 52 701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 133 297 728 753
Size: 129 4 164 431 25
Fragments arranged by size:
431 164 129 25 4
EcoO109I rG'GnC_Cy
Cuts at: 0 438 598 753
Size: 438 160 155
Fragments arranged by size:
438 160 155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 428 753
Size: 320 108 325
Fragments arranged by size:
325 320 108
NcoI C'CATG_G
Cuts at: 0 105 753
Size: 105 648
NdeI CA'TA_TG
Cuts at: 0 542 753
Size: 542 211
NspI r_CATG'y
Cuts at: 0 618 753
Size: 618 135
PflMI CCAn_nnn'nTGG
Cuts at: 0 111 753
Size: 111 642
PfoI T'CCnGG_A
Cuts at: 0 263 518 753
Size: 263 255 235
Fragments arranged by size:
263 255 235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 674 706 753
Size: 674 32 47
Fragments arranged by size:
674 47 32
PsiI TTA'TAA
Cuts at: 0 305 753
Size: 305 448
PspOMI G'GGCC_C
Cuts at: 0 101 753
Size: 101 652
PstI C_TGCA'G
Cuts at: 0 17 242 753
Size: 17 225 511
Fragments arranged by size:
511 225 17
SfcI C'TryA_G
Cuts at: 0 13 238 278 753
Size: 13 225 40 475
Fragments arranged by size:
475 225 40 13
SmaI CCC'GGG
Cuts at: 0 100 753
Size: 100 653
SphI G_CATG'C
Cuts at: 0 618 753
Size: 618 135
SrfI GCCC'GGGC
Cuts at: 0 100 753
Size: 100 653
StuI AGG'CCT
Cuts at: 0 82 526 753
Size: 82 444 227
Fragments arranged by size:
444 227 82
StyI C'CwwG_G
Cuts at: 0 92 105 441 753
Size: 92 13 336 312
Fragments arranged by size:
336 312 92 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 428 753
Size: 428 325
XmaI C'CCGG_G
Cuts at: 0 98 753
Size: 98 655
Enzymes that do cut:
AarI AcuI AloI AlwNI ApaI AvaI BanII BbvCI
BglI Bme1580I BmgBI BpmI Bpu10I BsaXI BsgI Bsp1286I
BspMI BsrFI Bsu36I BtgI BtgZI BtsI DrdI EciI
Eco57MI EcoO109I MmeI NcoI NdeI NspI PflMI PfoI
PpiI PsiI PspOMI PstI SfcI SmaI SphI SrfI
StuI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I ClaI CspCI DraI DraIII EaeI EagI EarI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpuMI PshAI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SspI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI