(Linear) (Six-base) MAPSORT of: KIEE5279.seq  Check: 662  from: 1  to: 714



KIEE5279.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:53  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     714 
   Size:        362     352
 
AhdI GACnn_n'nnGTC

Cuts at:      0     147     714 
   Size:        147     567
 
BanI G'GyrC_C

Cuts at:      0     304     714 
   Size:        304     410
 
BlpI GC'TnA_GC

Cuts at:      0      41     714 
   Size:         41     673
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     267     714 
   Size:         31     236     447

  Fragments arranged by size: 

                 447     236      31
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     520     714 
   Size:        349     171     194

  Fragments arranged by size: 

                 349     194     171
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     298     600     714 
   Size:        298     302     114

  Fragments arranged by size: 

                 302     298     114
 
BseYI C'CCAG_C

Cuts at:      0     440     714 
   Size:        440     274
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     160     714 
   Size:        160     554
 
BsrDI GCAATG_nn'

Cuts at:      0     342     714 
   Size:        342     372
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     705     714 
   Size:        705       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     267     362     714 
   Size:         31     236      95     352

  Fragments arranged by size: 

                 352     236      95      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     133     165     654     686     714 
   Size:        133      32     489      32      28

  Fragments arranged by size: 

                 489     133      32      32      28
 
HincII GTy'rAC

Cuts at:      0     152     714 
   Size:        152     562
 
HindIII A'AGCT_T

Cuts at:      0     467     714 
   Size:        467     247
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     606     714 
   Size:        606     108
 
MspA1I CmG'CkG

Cuts at:      0      24     310     714 
   Size:         24     286     404

  Fragments arranged by size: 

                 404     286      24
 
PvuII CAG'CTG

Cuts at:      0      24     714 
   Size:         24     690
 
SfcI C'TryA_G

Cuts at:      0     371     714 
   Size:        371     343
 
SmlI C'TyrA_G

Cuts at:      0     364     499     714 
   Size:        364     135     215

  Fragments arranged by size: 

                 364     215     135
 

 Enzymes that do cut:

     AcuI     AhdI     BanI     BlpI     BpmI    BpuEI    BseRI    BseYI
    BsmBI    BsrDI   BstAPI  Eco57MI    Hin4I   HincII  HindIII     MmeI
   MspA1I    PvuII     SfcI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI