Restriction map for
pFN21AE5278
(Linear) (Six-base) MAPSORT of: KIEE5278.seq Check: 6560 from: 1 to: 705
KIEE5278.orf
With 182 enzymes: SgfI *
January 29, 2014 11:53 ..
Acc65I G'GTAC_C
Cuts at: 0 180 705
Size: 180 525
AfeI AGC'GCT
Cuts at: 0 457 520 705
Size: 457 63 185
Fragments arranged by size:
457 185 63
AleI CACnn'nnGTG
Cuts at: 0 292 705
Size: 292 413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 281 705
Size: 281 424
AlwNI CAG_nnn'CTG
Cuts at: 0 129 597 705
Size: 129 468 108
Fragments arranged by size:
468 129 108
ApaLI G'TGCA_C
Cuts at: 0 256 705
Size: 256 449
AvaI C'yCGr_G
Cuts at: 0 614 705
Size: 614 91
BanI G'GyrC_C
Cuts at: 0 180 600 705
Size: 180 420 105
Fragments arranged by size:
420 180 105
BanII G_rGCy'C
Cuts at: 0 472 535 705
Size: 472 63 170
Fragments arranged by size:
472 170 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 144 178 705
Size: 144 34 527
Fragments arranged by size:
527 144 34
BglI GCCn_nnn'nGGC
Cuts at: 0 355 530 705
Size: 355 175 175
Fragments arranged by size:
355 175 175
Bme1580I G_kGCm'C
Cuts at: 0 260 705
Size: 260 445
BmgBI CAC'GTC
Cuts at: 0 339 705
Size: 339 366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 705
Size: 469 236
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 499 502 540 705
Size: 466 33 3 38 165
Fragments arranged by size:
466 165 38 33 3
BseYI C'CCAG_C
Cuts at: 0 75 122 416 705
Size: 75 47 294 289
Fragments arranged by size:
294 289 75 47
BsiEI CG_ry'CG
Cuts at: 0 164 631 705
Size: 164 467 74
Fragments arranged by size:
467 164 74
BsiHKAI G_wGCw'C
Cuts at: 0 260 705
Size: 260 445
Bsp1286I G_dGCh'C
Cuts at: 0 260 472 535 705
Size: 260 212 63 170
Fragments arranged by size:
260 212 170 63
BsrDI GCAATG_nn'
Cuts at: 0 12 705
Size: 12 693
BsrFI r'CCGG_y
Cuts at: 0 440 524 626 705
Size: 440 84 102 79
Fragments arranged by size:
440 102 84 79
BssHII G'CGCG_C
Cuts at: 0 495 497 646 705
Size: 495 2 149 59
Fragments arranged by size:
495 149 59 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 521 705
Size: 521 184
BstXI CCAn_nnnn'nTGG
Cuts at: 0 66 705
Size: 66 639
Bsu36I CC'TnA_GG
Cuts at: 0 176 705
Size: 176 529
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 354 705
Size: 354 351
BtsI GCAGTG_nn'
Cuts at: 0 222 258 705
Size: 222 36 447
Fragments arranged by size:
447 222 36
EaeI y'GGCC_r
Cuts at: 0 628 705
Size: 628 77
EagI C'GGCC_G
Cuts at: 0 628 705
Size: 628 77
EarI CTCTTCn'nnn_
Cuts at: 0 401 705
Size: 401 304
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 543 705
Size: 543 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 705
Size: 469 236
EcoRV GAT'ATC
Cuts at: 0 141 705
Size: 141 564
HaeII r_GCGC'y
Cuts at: 0 459 522 597 705
Size: 459 63 75 108
Fragments arranged by size:
459 108 75 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 705
Size: 23 32 650
Fragments arranged by size:
650 32 23
HindIII A'AGCT_T
Cuts at: 0 433 705
Size: 433 272
KpnI G_GTAC'C
Cuts at: 0 184 705
Size: 184 521
MslI CAynn'nnrTG
Cuts at: 0 292 705
Size: 292 413
MspA1I CmG'CkG
Cuts at: 0 75 447 510 633 639 705
Size: 75 372 63 123 6 66
Fragments arranged by size:
372 123 75 66 63 6
NaeI GCC'GGC
Cuts at: 0 442 526 705
Size: 442 84 179
Fragments arranged by size:
442 179 84
NgoMIV G'CCGG_C
Cuts at: 0 440 524 705
Size: 440 84 181
Fragments arranged by size:
440 181 84
PfoI T'CCnGG_A
Cuts at: 0 27 705
Size: 27 678
PstI C_TGCA'G
Cuts at: 0 137 593 705
Size: 137 456 112
Fragments arranged by size:
456 137 112
PvuII CAG'CTG
Cuts at: 0 75 447 705
Size: 75 372 258
Fragments arranged by size:
372 258 75
SfcI C'TryA_G
Cuts at: 0 133 589 705
Size: 133 456 116
Fragments arranged by size:
456 133 116
SmaI CCC'GGG
Cuts at: 0 616 705
Size: 616 89
StyI C'CwwG_G
Cuts at: 0 17 705
Size: 17 688
XmaI C'CCGG_G
Cuts at: 0 614 705
Size: 614 91
Enzymes that do cut:
Acc65I AfeI AleI AlfI AlwNI ApaLI AvaI BanI
BanII BcgI BglI Bme1580I BmgBI BpmI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BsrDI BsrFI BssHII BstAPI BstXI
Bsu36I BtgZI BtsI EaeI EagI EarI EciI Eco57MI
EcoRV HaeII Hin4I HindIII KpnI MslI MspA1I NaeI
NgoMIV PfoI PstI PvuII SfcI SmaI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AflIII AgeI
AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrGI BssSI BstBI
BstEII BstYI BstZ17I BtgI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HincII HpaI KasI MfeI MluI MmeI MscI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI