Restriction map for
pFN21AE5275
(Linear) (Six-base) MAPSORT of: KIEE5275.seq Check: 6567 from: 1 to: 651
KIEE5275.orf
With 182 enzymes: SgfI *
December 5, 2014 13:00 ..
AatII G_ACGT'C
Cuts at: 0 22 651
Size: 22 629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 439 558 651
Size: 208 231 119 93
Fragments arranged by size:
231 208 119 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 331 651
Size: 331 320
BanI G'GyrC_C
Cuts at: 0 388 651
Size: 388 263
BanII G_rGCy'C
Cuts at: 0 344 651
Size: 344 307
BbeI G_GCGC'C
Cuts at: 0 392 651
Size: 392 259
BbsI GAAGACnn'nnnn_
Cuts at: 0 357 651
Size: 357 294
BglII A'GATC_T
Cuts at: 0 488 651
Size: 488 163
BmrI ACTGGGnnnn_n'
Cuts at: 0 491 651
Size: 491 160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 651
Size: 358 293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 651
Size: 532 119
BsaHI Gr'CG_yC
Cuts at: 0 19 389 651
Size: 19 370 262
Fragments arranged by size:
370 262 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 651
Size: 184 30 437
Fragments arranged by size:
437 184 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 651
Size: 343 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 637 651
Size: 367 270 14
Fragments arranged by size:
367 270 14
Bsp1286I G_dGCh'C
Cuts at: 0 344 651
Size: 344 307
BsrBI CCG'CTC
Cuts at: 0 626 651
Size: 626 25
BstXI CCAn_nnnn'nTGG
Cuts at: 0 378 651
Size: 378 273
BstYI r'GATC_y
Cuts at: 0 488 651
Size: 488 163
Bsu36I CC'TnA_GG
Cuts at: 0 355 651
Size: 355 296
BtsI GCAGTG_nn'
Cuts at: 0 78 541 625 651
Size: 78 463 84 26
Fragments arranged by size:
463 84 78 26
DrdI GACnn_nn'nnGTC
Cuts at: 0 403 651
Size: 403 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 358 439 558 651
Size: 208 150 81 119 93
Fragments arranged by size:
208 150 119 93 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 96 651
Size: 96 555
EcoO109I rG'GnC_Cy
Cuts at: 0 423 651
Size: 423 228
FspI TGC'GCA
Cuts at: 0 610 651
Size: 610 41
HaeII r_GCGC'y
Cuts at: 0 392 651
Size: 392 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 480 508 512 540 651
Size: 480 28 4 28 111
Fragments arranged by size:
480 111 28 28 4
KasI G'GCGC_C
Cuts at: 0 388 651
Size: 388 263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 287 651
Size: 277 10 364
Fragments arranged by size:
364 277 10
MslI CAynn'nnrTG
Cuts at: 0 533 651
Size: 533 118
MspA1I CmG'CkG
Cuts at: 0 144 641 651
Size: 144 497 10
Fragments arranged by size:
497 144 10
NarI GG'CG_CC
Cuts at: 0 389 651
Size: 389 262
NdeI CA'TA_TG
Cuts at: 0 150 651
Size: 150 501
PfoI T'CCnGG_A
Cuts at: 0 13 651
Size: 13 638
PpuMI rG'GwC_Cy
Cuts at: 0 423 651
Size: 423 228
PstI C_TGCA'G
Cuts at: 0 9 215 651
Size: 9 206 436
Fragments arranged by size:
436 206 9
PvuII CAG'CTG
Cuts at: 0 144 641 651
Size: 144 497 10
Fragments arranged by size:
497 144 10
SfcI C'TryA_G
Cuts at: 0 5 211 504 651
Size: 5 206 293 147
Fragments arranged by size:
293 206 147 5
SfoI GGC'GCC
Cuts at: 0 390 651
Size: 390 261
SmlI C'TyrA_G
Cuts at: 0 547 651
Size: 547 104
Tth111I GACn'n_nGTC
Cuts at: 0 592 651
Size: 592 59
ZraI GAC'GTC
Cuts at: 0 20 651
Size: 20 631
Enzymes that do cut:
AatII AcuI AlfI BanI BanII BbeI BbsI BglII
BmrI BpmI BpuEI BsaHI BsaXI BseRI BsgI Bsp1286I
BsrBI BstXI BstYI Bsu36I BtsI DrdI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KasI MmeI MslI MspA1I
NarI NdeI PfoI PpuMI PstI PvuII SfcI SfoI
SmlI Tth111I ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaWI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII EaeI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI NaeI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI TstI XbaI XcmI XhoI XmaI
XmnI