(Linear) (Six-base) MAPSORT of: KIEE5273.seq  Check: 672  from: 1  to: 534



KIEE5273.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:53  ..

AflIII A'CryG_T

Cuts at:      0     173     534 
   Size:        173     361
 
AlwNI CAG_nnn'CTG

Cuts at:      0     245     534 
   Size:        245     289
 
ApoI r'AATT_y

Cuts at:      0      42     534 
   Size:         42     492
 
BanI G'GyrC_C

Cuts at:      0     133     534 
   Size:        133     401
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      41     534 
   Size:         41     493
 
BbvCI CC'TCA_GC

Cuts at:      0     238     534 
   Size:        238     296
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     472     506     534 
   Size:        472      34      28

  Fragments arranged by size: 

                 472      34      28
 
BglII A'GATC_T

Cuts at:      0     290     534 
   Size:        290     244
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     399     534 
   Size:        399     135
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     534 
   Size:        418     116
 
Bpu10I CC'TnA_GC

Cuts at:      0     238     534 
   Size:        238     296
 
BsaBI GATnn'nnATC

Cuts at:      0     289     534 
   Size:        289     245
 
BsaWI w'CCGG_w

Cuts at:      0     470     534 
   Size:        470      64
 
BseYI C'CCAG_C

Cuts at:      0     166     501     534 
   Size:        166     335      33

  Fragments arranged by size: 

                 335     166      33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     512     534 
   Size:        101     411      22

  Fragments arranged by size: 

                 411     101      22
 
BsiEI CG_ry'CG

Cuts at:      0     234     534 
   Size:        234     300
 
BsrGI T'GTAC_A

Cuts at:      0     176     534 
   Size:        176     358
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     123     534 
   Size:        123     411
 
BstYI r'GATC_y

Cuts at:      0     290     377     534 
   Size:        290      87     157

  Fragments arranged by size: 

                 290     157      87
 
BtgI C'CryG_G

Cuts at:      0     435     534 
   Size:        435      99
 
ClaI AT'CG_AT

Cuts at:      0     482     534 
   Size:        482      52
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     417     452     534 
   Size:        417      35      82

  Fragments arranged by size: 

                 417      82      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     508     534 
   Size:        508      26
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     534 
   Size:        418     116
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     269     534 
   Size:        269     265
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     534 
   Size:        196     338
 
MslI CAynn'nnrTG

Cuts at:      0     121     534 
   Size:        121     413
 
MspA1I CmG'CkG

Cuts at:      0     505     534 
   Size:        505      29
 
NspI r_CATG'y

Cuts at:      0     177     372     534 
   Size:        177     195     162

  Fragments arranged by size: 

                 195     177     162
 
PciI A'CATG_T

Cuts at:      0     173     534 
   Size:        173     361
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     485     534 
   Size:        485      49
 
PfoI T'CCnGG_A

Cuts at:      0     452     534 
   Size:        452      82
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0       6      38     534 
   Size:          6      32     496

  Fragments arranged by size: 

                 496      32       6
 
PstI C_TGCA'G

Cuts at:      0     250     534 
   Size:        250     284
 
PvuII CAG'CTG

Cuts at:      0     505     534 
   Size:        505      29
 
SfcI C'TryA_G

Cuts at:      0     246     534 
   Size:        246     288
 
TatI w'GTAC_w

Cuts at:      0     176     534 
   Size:        176     358
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI     BanI     BbsI    BbvCI     BcgI    BglII
     BplI     BpmI   Bpu10I    BsaBI    BsaWI    BseYI     BsgI    BsiEI
    BsrGI    BstXI    BstYI     BtgI     ClaI    CspCI     EciI  Eco57MI
    EcoNI     MmeI     MslI   MspA1I     NspI     PciI    PflMI     PfoI
     PsrI     PstI    PvuII     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI