(Linear) (Six-base) MAPSORT of: KIEE5272.seq  Check: 8588  from: 1  to: 531



KIEE5272.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:53  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     253     531 
   Size:        105     148     278

  Fragments arranged by size: 

                 278     148     105
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     480     513     531 
   Size:        480      33      18

  Fragments arranged by size: 

                 480      33      18
 
BanII G_rGCy'C

Cuts at:      0      57     170     531 
   Size:         57     113     361

  Fragments arranged by size: 

                 361     113      57
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     501     531 
   Size:        501      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     531 
   Size:        280     251
 
Bpu10I CC'TnA_GC

Cuts at:      0     161     531 
   Size:        161     370
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     531 
   Size:         32     499
 
BsiHKAI G_wGCw'C

Cuts at:      0      57     531 
   Size:         57     474
 
Bsp1286I G_dGCh'C

Cuts at:      0      57     170     531 
   Size:         57     113     361

  Fragments arranged by size: 

                 361     113      57
 
EaeI y'GGCC_r

Cuts at:      0     294     531 
   Size:        294     237
 
EcoICRI GAG'CTC

Cuts at:      0      55     531 
   Size:         55     476
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     253     280     531 
   Size:        105     148      27     251

  Fragments arranged by size: 

                 251     148     105      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     360     392     531 
   Size:        360      32     139

  Fragments arranged by size: 

                 360     139      32
 
MscI TGG'CCA

Cuts at:      0     296     531 
   Size:        296     235
 
NspI r_CATG'y

Cuts at:      0     430     531 
   Size:        430     101
 
PfoI T'CCnGG_A

Cuts at:      0      67     531 
   Size:         67     464
 
SacI G_AGCT'C

Cuts at:      0      57     531 
   Size:         57     474
 
SmlI C'TyrA_G

Cuts at:      0      47     531 
   Size:         47     484
 

 Enzymes that do cut:

     AcuI     BaeI    BanII     BmrI     BpmI   Bpu10I    BpuEI  BsiHKAI
 Bsp1286I     EaeI  EcoICRI  Eco57MI    Hin4I     MscI     NspI     PfoI
     SacI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI