Restriction map for
pFN21AE5269
(Linear) (Six-base) MAPSORT of: KIEE5269.seq Check: 9623 from: 1 to: 594
KIEE5269.orf
With 182 enzymes: SgfI *
January 29, 2014 11:53 ..
AclI AA'CG_TT
Cuts at: 0 265 594
Size: 265 329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 594
Size: 277 317
AlwNI CAG_nnn'CTG
Cuts at: 0 198 594
Size: 198 396
ApaLI G'TGCA_C
Cuts at: 0 149 594
Size: 149 445
BanI G'GyrC_C
Cuts at: 0 81 577 594
Size: 81 496 17
Fragments arranged by size:
496 81 17
BanII G_rGCy'C
Cuts at: 0 254 594
Size: 254 340
BbvCI CC'TCA_GC
Cuts at: 0 536 594
Size: 536 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 453 487 594
Size: 453 34 107
Fragments arranged by size:
453 107 34
BclI T'GATC_A
Cuts at: 0 434 594
Size: 434 160
BglI GCCn_nnn'nGGC
Cuts at: 0 343 565 594
Size: 343 222 29
Fragments arranged by size:
343 222 29
Bme1580I G_kGCm'C
Cuts at: 0 153 594
Size: 153 441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 594
Size: 583 11
Bpu10I CC'TnA_GC
Cuts at: 0 536 594
Size: 536 58
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 126 594
Size: 126 468
BsiEI CG_ry'CG
Cuts at: 0 14 594
Size: 14 580
BsiHKAI G_wGCw'C
Cuts at: 0 153 254 594
Size: 153 101 340
Fragments arranged by size:
340 153 101
Bsp1286I G_dGCh'C
Cuts at: 0 153 254 594
Size: 153 101 340
Fragments arranged by size:
340 153 101
BsrBI CCG'CTC
Cuts at: 0 16 594
Size: 16 578
BsrFI r'CCGG_y
Cuts at: 0 6 594
Size: 6 588
BstYI r'GATC_y
Cuts at: 0 272 594
Size: 272 322
BtgI C'CryG_G
Cuts at: 0 416 581 594
Size: 416 165 13
Fragments arranged by size:
416 165 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 195 594
Size: 195 399
EaeI y'GGCC_r
Cuts at: 0 11 120 594
Size: 11 109 474
Fragments arranged by size:
474 109 11
EagI C'GGCC_G
Cuts at: 0 11 594
Size: 11 583
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 52 385 556 594
Size: 52 333 171 38
Fragments arranged by size:
333 171 52 38
EcoICRI GAG'CTC
Cuts at: 0 252 594
Size: 252 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 583 594
Size: 277 306 11
Fragments arranged by size:
306 277 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 403 475 505 594
Size: 403 72 30 89
Fragments arranged by size:
403 89 72 30
EcoO109I rG'GnC_Cy
Cuts at: 0 31 138 594
Size: 31 107 456
Fragments arranged by size:
456 107 31
EcoRV GAT'ATC
Cuts at: 0 360 594
Size: 360 234
MspA1I CmG'CkG
Cuts at: 0 55 119 198 594
Size: 55 64 79 396
Fragments arranged by size:
396 79 64 55
NaeI GCC'GGC
Cuts at: 0 8 594
Size: 8 586
NdeI CA'TA_TG
Cuts at: 0 107 594
Size: 107 487
NgoMIV G'CCGG_C
Cuts at: 0 6 594
Size: 6 588
NotI GC'GGCC_GC
Cuts at: 0 11 594
Size: 11 583
PfoI T'CCnGG_A
Cuts at: 0 203 275 594
Size: 203 72 319
Fragments arranged by size:
319 203 72
PstI C_TGCA'G
Cuts at: 0 333 594
Size: 333 261
PvuII CAG'CTG
Cuts at: 0 198 594
Size: 198 396
SacI G_AGCT'C
Cuts at: 0 254 594
Size: 254 340
SfcI C'TryA_G
Cuts at: 0 329 594
Size: 329 265
StyI C'CwwG_G
Cuts at: 0 39 170 459 594
Size: 39 131 289 135
Fragments arranged by size:
289 135 131 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 289 418 594
Size: 289 129 176
Fragments arranged by size:
289 176 129
Enzymes that do cut:
AclI AcuI AlwNI ApaLI BanI BanII BbvCI BcgI
BclI BglI Bme1580I BpmI Bpu10I BseRI BsiEI BsiHKAI
Bsp1286I BsrBI BsrFI BstYI BtgI BtgZI EaeI EagI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV MspA1I NaeI
NdeI NgoMIV NotI PfoI PstI PvuII SacI SfcI
StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BciVI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EarI EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NarI NcoI NheI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI