(Linear) (Six-base) MAPSORT of: KIEE5267.seq  Check: 1197  from: 1  to: 564



KIEE5267.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:53  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     564 
   Size:        178     386
 
AflII C'TTAA_G

Cuts at:      0     550     564 
   Size:        550      14
 
AlwNI CAG_nnn'CTG

Cuts at:      0     298     564 
   Size:        298     266
 
ApoI r'AATT_y

Cuts at:      0     172     192     564 
   Size:        172      20     372

  Fragments arranged by size: 

                 372     172      20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     146     564 
   Size:        146     418
 
BclI T'GATC_A

Cuts at:      0     234     564 
   Size:        234     330
 
BglII A'GATC_T

Cuts at:      0      81     322     564 
   Size:         81     241     242

  Fragments arranged by size: 

                 242     241      81
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     360     564 
   Size:        360     204
 
BsaBI GATnn'nnATC

Cuts at:      0     233     564 
   Size:        233     331
 
BsaWI w'CCGG_w

Cuts at:      0     109     564 
   Size:        109     455
 
BspEI T'CCGG_A

Cuts at:      0     109     564 
   Size:        109     455
 
BssSI C'ACGA_G

Cuts at:      0     185     564 
   Size:        185     379
 
BstYI r'GATC_y

Cuts at:      0      81     204     322     564 
   Size:         81     123     118     242

  Fragments arranged by size: 

                 242     123     118      81
 
BtgI C'CryG_G

Cuts at:      0     332     564 
   Size:        332     232
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     564 
   Size:        178     386
 
EcoRI G'AATT_C

Cuts at:      0     172     564 
   Size:        172     392
 
EcoRV GAT'ATC

Cuts at:      0     218     564 
   Size:        218     346
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     357     389     564 
   Size:        357      32     175

  Fragments arranged by size: 

                 357     175      32
 
HincII GTy'rAC

Cuts at:      0     287     564 
   Size:        287     277
 
NcoI C'CATG_G

Cuts at:      0     332     564 
   Size:        332     232
 
SfcI C'TryA_G

Cuts at:      0      35     134     290     564 
   Size:         35      99     156     274

  Fragments arranged by size: 

                 274     156      99      35
 
SmlI C'TyrA_G

Cuts at:      0     550     564 
   Size:        550      14
 
StyI C'CwwG_G

Cuts at:      0     332     564 
   Size:        332     232
 
Tth111I GACn'n_nGTC

Cuts at:      0     371     564 
   Size:        371     193
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI     ApoI     BbsI     BclI    BglII     BsaI
    BsaBI    BsaWI    BspEI    BssSI    BstYI     BtgI  Eco57MI    EcoRI
    EcoRV    Hin4I   HincII     NcoI     SfcI     SmlI     StyI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI