(Linear) (Six-base) MAPSORT of: KIEE5265.seq  Check: 2560  from: 1  to: 570



KIEE5265.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:53  ..

ApoI r'AATT_y

Cuts at:      0     469     570 
   Size:        469     101
 
BanI G'GyrC_C

Cuts at:      0     210     570 
   Size:        210     360
 
BclI T'GATC_A

Cuts at:      0     503     570 
   Size:        503      67
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     570 
   Size:        344     226
 
BsaAI yAC'GTr

Cuts at:      0     553     570 
   Size:        553      17
 
BsaHI Gr'CG_yC

Cuts at:      0     392     570 
   Size:        392     178
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      49      79     570 
   Size:         49      30     491

  Fragments arranged by size: 

                 491      49      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     570 
   Size:        295     275
 
BsiWI C'GTAC_G

Cuts at:      0     301     570 
   Size:        301     269
 
BsmI GAATG_Cn'

Cuts at:      0     194     570 
   Size:        194     376
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     244     570 
   Size:        244     326
 
BspHI T'CATG_A

Cuts at:      0     362     570 
   Size:        362     208
 
BsrDI GCAATG_nn'

Cuts at:      0     102     260     570 
   Size:        102     158     310

  Fragments arranged by size: 

                 310     158     102
 
BsrFI r'CCGG_y

Cuts at:      0     513     570 
   Size:        513      57
 
BssSI C'ACGA_G

Cuts at:      0     227     570 
   Size:        227     343
 
BtsI GCAGTG_nn'

Cuts at:      0     312     570 
   Size:        312     258
 
ClaI AT'CG_AT

Cuts at:      0     284     570 
   Size:        284     286
 
EaeI y'GGCC_r

Cuts at:      0     104     570 
   Size:        104     466
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     327     570 
   Size:        327     243
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     570 
   Size:        344     226
 
EcoO109I rG'GnC_Cy

Cuts at:      0     546     570 
   Size:        546      24
 
EcoRI G'AATT_C

Cuts at:      0     469     570 
   Size:        469     101
 
EcoRV GAT'ATC

Cuts at:      0     563     570 
   Size:        563       7
 
FspI TGC'GCA

Cuts at:      0     499     570 
   Size:        499      71
 
FspAI rTGC'GCAy

Cuts at:      0     499     570 
   Size:        499      71
 
HincII GTy'rAC

Cuts at:      0     177     570 
   Size:        177     393
 
HindIII A'AGCT_T

Cuts at:      0      81     570 
   Size:         81     489
 
MslI CAynn'nnrTG

Cuts at:      0     208     286     570 
   Size:        208      78     284

  Fragments arranged by size: 

                 284     208      78
 
PpuMI rG'GwC_Cy

Cuts at:      0     546     570 
   Size:        546      24
 
PshAI GACnn'nnGTC

Cuts at:      0     236     570 
   Size:        236     334
 
SfcI C'TryA_G

Cuts at:      0      57     570 
   Size:         57     513
 
SnaBI TAC'GTA

Cuts at:      0     553     570 
   Size:        553      17
 
TatI w'GTAC_w

Cuts at:      0     200     570 
   Size:        200     370
 
Tth111I GACn'n_nGTC

Cuts at:      0     408     570 
   Size:        408     162
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     384     570 
   Size:        384     186
 

 Enzymes that do cut:

     ApoI     BanI     BclI     BpmI    BsaAI    BsaHI    BsaXI     BsgI
    BsiWI     BsmI    BsmBI    BspHI    BsrDI    BsrFI    BssSI     BtsI
     ClaI     EaeI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FspI
    FspAI   HincII  HindIII     MslI    PpuMI    PshAI     SfcI    SnaBI
     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI
     DraI   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI     FalI
     FseI    HaeII    Hin4I     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI     XbaI     XhoI     XmaI     XmnI
     ZraI