Restriction map for
pFN21AE5263
(Linear) (Six-base) MAPSORT of: KIEE5263.seq Check: 5309 from: 1 to: 516
KIEE5263.orf
With 182 enzymes: SgfI *
December 5, 2014 13:00 ..
Acc65I G'GTAC_C
Cuts at: 0 77 516
Size: 77 439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 138 516
Size: 138 378
AvaI C'yCGr_G
Cuts at: 0 22 516
Size: 22 494
BamHI G'GATC_C
Cuts at: 0 452 516
Size: 452 64
BanI G'GyrC_C
Cuts at: 0 18 77 225 516
Size: 18 59 148 291
Fragments arranged by size:
291 148 59 18
BbeI G_GCGC'C
Cuts at: 0 22 229 516
Size: 22 207 287
Fragments arranged by size:
287 207 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 300 516
Size: 300 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 360 394 516
Size: 360 34 122
Fragments arranged by size:
360 122 34
BclI T'GATC_A
Cuts at: 0 191 516
Size: 191 325
BmgBI CAC'GTC
Cuts at: 0 71 516
Size: 71 445
Bpu10I CC'TnA_GC
Cuts at: 0 9 516
Size: 9 507
BsaI GGTCTCn'nnnn_
Cuts at: 0 490 516
Size: 490 26
BsaHI Gr'CG_yC
Cuts at: 0 19 28 226 516
Size: 19 9 198 290
Fragments arranged by size:
290 198 19 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 496 516
Size: 496 20
BseYI C'CCAG_C
Cuts at: 0 108 366 516
Size: 108 258 150
Fragments arranged by size:
258 150 108
BsiEI CG_ry'CG
Cuts at: 0 179 329 516
Size: 179 150 187
Fragments arranged by size:
187 179 150
BsmI GAATG_Cn'
Cuts at: 0 204 516
Size: 204 312
BspHI T'CATG_A
Cuts at: 0 194 516
Size: 194 322
BsrFI r'CCGG_y
Cuts at: 0 178 281 516
Size: 178 103 235
Fragments arranged by size:
235 178 103
BstXI CCAn_nnnn'nTGG
Cuts at: 0 366 516
Size: 366 150
BstYI r'GATC_y
Cuts at: 0 452 516
Size: 452 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 156 363 446 516
Size: 156 207 83 70
Fragments arranged by size:
207 156 83 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 245 280 516
Size: 245 35 236
Fragments arranged by size:
245 236 35
EaeI y'GGCC_r
Cuts at: 0 176 516
Size: 176 340
EagI C'GGCC_G
Cuts at: 0 176 516
Size: 176 340
EarI CTCTTCn'nnn_
Cuts at: 0 53 145 516
Size: 53 92 371
Fragments arranged by size:
371 92 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 21 283 490 516
Size: 21 262 207 26
Fragments arranged by size:
262 207 26 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 447 516
Size: 447 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 324 516
Size: 324 192
HaeII r_GCGC'y
Cuts at: 0 22 229 516
Size: 22 207 287
Fragments arranged by size:
287 207 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 516
Size: 424 32 60
Fragments arranged by size:
424 60 32
KasI G'GCGC_C
Cuts at: 0 18 225 516
Size: 18 207 291
Fragments arranged by size:
291 207 18
KpnI G_GTAC'C
Cuts at: 0 81 516
Size: 81 435
MspA1I CmG'CkG
Cuts at: 0 83 516
Size: 83 433
NaeI GCC'GGC
Cuts at: 0 180 516
Size: 180 336
NarI GG'CG_CC
Cuts at: 0 19 226 516
Size: 19 207 290
Fragments arranged by size:
290 207 19
NgoMIV G'CCGG_C
Cuts at: 0 178 516
Size: 178 338
SfoI GGC'GCC
Cuts at: 0 20 227 516
Size: 20 207 289
Fragments arranged by size:
289 207 20
SmaI CCC'GGG
Cuts at: 0 24 516
Size: 24 492
XmaI C'CCGG_G
Cuts at: 0 22 516
Size: 22 494
XmnI GAAnn'nnTTC
Cuts at: 0 202 516
Size: 202 314
Enzymes that do cut:
Acc65I AlfI AvaI BamHI BanI BbeI BbsI BcgI
BclI BmgBI Bpu10I BsaI BsaHI BseRI BseYI BsiEI
BsmI BspHI BsrFI BstXI BstYI BtgZI CspCI EaeI
EagI EarI EciI EcoNI FalI HaeII Hin4I KasI
KpnI MspA1I NaeI NarI NgoMIV SfoI SmaI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BanII BbvCI BciVI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtsI ClaI DraI DraIII DrdI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
MfeI MluI MmeI MscI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI ZraI