Restriction map for
pFN21AE5262
(Linear) (Six-base) MAPSORT of: KIEE5262.seq Check: 1489 from: 1 to: 537
KIEE5262.orf
With 182 enzymes: SgfI *
January 29, 2014 11:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 537
Size: 436 101
AflIII A'CryG_T
Cuts at: 0 413 537
Size: 413 124
AlwNI CAG_nnn'CTG
Cuts at: 0 434 537
Size: 434 103
ApoI r'AATT_y
Cuts at: 0 463 537
Size: 463 74
BanI G'GyrC_C
Cuts at: 0 73 537
Size: 73 464
BanII G_rGCy'C
Cuts at: 0 308 537
Size: 308 229
BbeI G_GCGC'C
Cuts at: 0 77 537
Size: 77 460
BbsI GAAGACnn'nnnn_
Cuts at: 0 315 537
Size: 315 222
BbvCI CC'TCA_GC
Cuts at: 0 17 113 537
Size: 17 96 424
Fragments arranged by size:
424 96 17
BmgBI CAC'GTC
Cuts at: 0 315 537
Size: 315 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 537
Size: 63 474
Bpu10I CC'TnA_GC
Cuts at: 0 17 113 302 537
Size: 17 96 189 235
Fragments arranged by size:
235 189 96 17
BsaI GGTCTCn'nnnn_
Cuts at: 0 190 537
Size: 190 347
BsaHI Gr'CG_yC
Cuts at: 0 74 254 537
Size: 74 180 283
Fragments arranged by size:
283 180 74
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 537
Size: 247 290
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 312 537
Size: 244 68 225
Fragments arranged by size:
244 225 68
BsiEI CG_ry'CG
Cuts at: 0 159 537
Size: 159 378
BsiHKAI G_wGCw'C
Cuts at: 0 308 537
Size: 308 229
Bsp1286I G_dGCh'C
Cuts at: 0 308 537
Size: 308 229
BsrBI CCG'CTC
Cuts at: 0 239 537
Size: 239 298
BsrFI r'CCGG_y
Cuts at: 0 154 158 537
Size: 154 4 379
Fragments arranged by size:
379 154 4
BstYI r'GATC_y
Cuts at: 0 398 537
Size: 398 139
BtgI C'CryG_G
Cuts at: 0 68 353 468 537
Size: 68 285 115 69
Fragments arranged by size:
285 115 69 68
DraIII CAC_nnn'GTG
Cuts at: 0 267 537
Size: 267 270
DrdI GACnn_nn'nnGTC
Cuts at: 0 319 537
Size: 319 218
EaeI y'GGCC_r
Cuts at: 0 156 537
Size: 156 381
EagI C'GGCC_G
Cuts at: 0 156 537
Size: 156 381
EcoICRI GAG'CTC
Cuts at: 0 306 537
Size: 306 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 436 537
Size: 63 373 101
Fragments arranged by size:
373 101 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 537
Size: 262 275
EcoO109I rG'GnC_Cy
Cuts at: 0 347 537
Size: 347 190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 162 537
Size: 162 375
FseI GG_CCGG'CC
Cuts at: 0 158 537
Size: 158 379
HaeII r_GCGC'y
Cuts at: 0 77 537
Size: 77 460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 185 217 537
Size: 185 32 320
Fragments arranged by size:
320 185 32
KasI G'GCGC_C
Cuts at: 0 73 537
Size: 73 464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 537
Size: 25 512
MslI CAynn'nnrTG
Cuts at: 0 313 537
Size: 313 224
MspA1I CmG'CkG
Cuts at: 0 390 537
Size: 390 147
NaeI GCC'GGC
Cuts at: 0 156 537
Size: 156 381
NarI GG'CG_CC
Cuts at: 0 74 537
Size: 74 463
NcoI C'CATG_G
Cuts at: 0 68 537
Size: 68 469
NgoMIV G'CCGG_C
Cuts at: 0 154 537
Size: 154 383
NspI r_CATG'y
Cuts at: 0 322 417 537
Size: 322 95 120
Fragments arranged by size:
322 120 95
PasI CC'CwG_GG
Cuts at: 0 61 537
Size: 61 476
PciI A'CATG_T
Cuts at: 0 413 537
Size: 413 124
PfoI T'CCnGG_A
Cuts at: 0 503 537
Size: 503 34
PpuMI rG'GwC_Cy
Cuts at: 0 347 537
Size: 347 190
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 466 498 537
Size: 466 32 39
Fragments arranged by size:
466 39 32
PstI C_TGCA'G
Cuts at: 0 110 230 537
Size: 110 120 307
Fragments arranged by size:
307 120 110
PvuII CAG'CTG
Cuts at: 0 390 537
Size: 390 147
SacI G_AGCT'C
Cuts at: 0 308 537
Size: 308 229
SanDI GG'GwC_CC
Cuts at: 0 347 537
Size: 347 190
SfcI C'TryA_G
Cuts at: 0 106 226 537
Size: 106 120 311
Fragments arranged by size:
311 120 106
SfoI GGC'GCC
Cuts at: 0 75 537
Size: 75 462
StyI C'CwwG_G
Cuts at: 0 68 537
Size: 68 469
Tth111I GACn'n_nGTC
Cuts at: 0 532 537
Size: 532 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 41 537
Size: 41 496
XmnI GAAnn'nnTTC
Cuts at: 0 463 537
Size: 463 74
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BanI BanII BbeI BbsI
BbvCI BmgBI BpmI Bpu10I BsaI BsaHI BseRI BsgI
BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BstYI BtgI DraIII
DrdI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI HaeII Hin4I KasI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PpuMI
PsrI PstI PvuII SacI SanDI SfcI SfoI StyI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaWI BsaXI BseYI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI EarI EciI EcoRI EcoRV FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NdeI NheI NotI NruI NsiI PacI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI XbaI XhoI XmaI
ZraI