(Linear) (Six-base) MAPSORT of: KIEE5253.seq  Check: 6474  from: 1  to: 597



KIEE5253.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:53  ..

AatII G_ACGT'C

Cuts at:      0     380     597 
   Size:        380     217
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     597 
   Size:         52     545
 
AleI CACnn'nnGTG

Cuts at:      0     535     597 
   Size:        535      62
 
AlwNI CAG_nnn'CTG

Cuts at:      0     226     573     597 
   Size:        226     347      24

  Fragments arranged by size: 

                 347     226      24
 
ApoI r'AATT_y

Cuts at:      0     189     349     597 
   Size:        189     160     248

  Fragments arranged by size: 

                 248     189     160
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     316     404     597 
   Size:        316      88     193

  Fragments arranged by size: 

                 316     193      88
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     140     597 
   Size:        140     457
 
BglI GCCn_nnn'nGGC

Cuts at:      0      97     518     597 
   Size:         97     421      79

  Fragments arranged by size: 

                 421      97      79
 
Bme1580I G_kGCm'C

Cuts at:      0     532     597 
   Size:        532      65
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     442     597 
   Size:        343      99     155

  Fragments arranged by size: 

                 343     155      99
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     216     597 
   Size:        216     381
 
BsaHI Gr'CG_yC

Cuts at:      0     377     597 
   Size:        377     220
 
BsiHKAI G_wGCw'C

Cuts at:      0     359     597 
   Size:        359     238
 
Bsp1286I G_dGCh'C

Cuts at:      0     359     532     597 
   Size:        359     173      65

  Fragments arranged by size: 

                 359     173      65
 
BsrFI r'CCGG_y

Cuts at:      0     517     597 
   Size:        517      80
 
BssSI C'ACGA_G

Cuts at:      0     531     597 
   Size:        531      66
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     226     597 
   Size:        226     371
 
BstEII G'GTnAC_C

Cuts at:      0     261     597 
   Size:        261     336
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     273     321     597 
   Size:        273      48     276

  Fragments arranged by size: 

                 276     273      48
 
BtgI C'CryG_G

Cuts at:      0     524     597 
   Size:        524      73
 
BtsI GCAGTG_nn'

Cuts at:      0     227     597 
   Size:        227     370
 
EaeI y'GGCC_r

Cuts at:      0      41     597 
   Size:         41     556
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     157     562     597 
   Size:        157     405      35

  Fragments arranged by size: 

                 405     157      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     343     442     597 
   Size:         52     291      99     155

  Fragments arranged by size: 

                 291     155      99      52
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     303     335     547     579     597 
   Size:        303      32     212      32      18

  Fragments arranged by size: 

                 303     212      32      32      18
 
MslI CAynn'nnrTG

Cuts at:      0     535     597 
   Size:        535      62
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     465     597 
   Size:        465     132
 
PfoI T'CCnGG_A

Cuts at:      0     458     597 
   Size:        458     139
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      97     597 
   Size:         97     500
 
SmlI C'TyrA_G

Cuts at:      0     195     597 
   Size:        195     402
 
Tth111I GACn'n_nGTC

Cuts at:      0     267     597 
   Size:        267     330
 
ZraI GAC'GTC

Cuts at:      0     378     597 
   Size:        378     219
 

 Enzymes that do cut:

    AatII     AcuI     AleI    AlwNI     ApoI     BbsI    BciVI     BglI
 Bme1580I     BpmI    BpuEI    BsaHI  BsiHKAI Bsp1286I    BsrFI    BssSI
   BstAPI   BstEII    BstXI     BtgI     BtsI     EaeI     EciI  Eco57MI
    Hin4I     MslI    PflMI     PfoI     SfiI     SmlI  Tth111I     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BstBI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI