(Linear) (Six-base) MAPSORT of: KIEE5251.seq  Check: 3226  from: 1  to: 540



KIEE5251.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AflIII A'CryG_T

Cuts at:      0     492     540 
   Size:        492      48
 
ApoI r'AATT_y

Cuts at:      0     464     540 
   Size:        464      76
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     336     370     540 
   Size:        336      34     170

  Fragments arranged by size: 

                 336     170      34
 
BlpI GC'TnA_GC

Cuts at:      0      46     540 
   Size:         46     494
 
BmgBI CAC'GTC

Cuts at:      0     126     540 
   Size:        126     414
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     540 
   Size:        391     149
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     409     540 
   Size:        409     131
 
BsaAI yAC'GTr

Cuts at:      0     495     540 
   Size:        495      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     540 
   Size:        106     434
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     132     540 
   Size:        132     408
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     106     540 
   Size:        106     434
 
EarI CTCTTCn'nnn_

Cuts at:      0     526     540 
   Size:        526      14
 
EcoRI G'AATT_C

Cuts at:      0     464     540 
   Size:        464      76
 
HaeII r_GCGC'y

Cuts at:      0      64     540 
   Size:         64     476
 
NdeI CA'TA_TG

Cuts at:      0     519     540 
   Size:        519      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     460     540 
   Size:        460      80
 
SmlI C'TyrA_G

Cuts at:      0     406     540 
   Size:        406     134
 
SspI AAT'ATT

Cuts at:      0     277     379     540 
   Size:        277     102     161

  Fragments arranged by size: 

                 277     161     102
 
Tth111I GACn'n_nGTC

Cuts at:      0     435     540 
   Size:        435     105
 
XbaI T'CTAG_A

Cuts at:      0     219     540 
   Size:        219     321
 

 Enzymes that do cut:

   AflIII     ApoI     BcgI     BlpI    BmgBI    BpuEI     BsaI    BsaAI
     BsgI    BsmBI     DrdI     EarI    EcoRI    HaeII     NdeI    PflMI
     SmlI     SspI  Tth111I     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI     TstI     XcmI     XhoI     XmaI     XmnI     ZraI