(Linear) (Six-base) MAPSORT of: KIEE5250.seq  Check: 1378  from: 1  to: 582



KIEE5250.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     246     421     582 
   Size:        246     175     161

  Fragments arranged by size: 

                 246     175     161
 
AlwNI CAG_nnn'CTG

Cuts at:      0      82     582 
   Size:         82     500
 
AvaI C'yCGr_G

Cuts at:      0     231     582 
   Size:        231     351
 
BanI G'GyrC_C

Cuts at:      0      91     184     582 
   Size:         91      93     398

  Fragments arranged by size: 

                 398      93      91
 
BbeI G_GCGC'C

Cuts at:      0     188     582 
   Size:        188     394
 
BglI GCCn_nnn'nGGC

Cuts at:      0      85     582 
   Size:         85     497
 
BlpI GC'TnA_GC

Cuts at:      0     432     582 
   Size:        432     150
 
BmgBI CAC'GTC

Cuts at:      0     423     582 
   Size:        423     159
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     264     582 
   Size:        264     318
 
Bpu10I CC'TnA_GC

Cuts at:      0      16     582 
   Size:         16     566
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     539     582 
   Size:        539      43
 
BsaHI Gr'CG_yC

Cuts at:      0     185     582 
   Size:        185     397
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     582 
   Size:        405     177
 
BsmI GAATG_Cn'

Cuts at:      0     152     582 
   Size:        152     430
 
BsrBI CCG'CTC

Cuts at:      0     302     582 
   Size:        302     280
 
BstYI r'GATC_y

Cuts at:      0     278     582 
   Size:        278     304
 
BtsI GCAGTG_nn'

Cuts at:      0      51     414     582 
   Size:         51     363     168

  Fragments arranged by size: 

                 363     168      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     246     421     582 
   Size:        246     175     161

  Fragments arranged by size: 

                 246     175     161
 
HaeII r_GCGC'y

Cuts at:      0     188     582 
   Size:        188     394
 
KasI G'GCGC_C

Cuts at:      0     184     582 
   Size:        184     398
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     582 
   Size:        578       4
 
NarI GG'CG_CC

Cuts at:      0     185     582 
   Size:        185     397
 
PfoI T'CCnGG_A

Cuts at:      0     329     582 
   Size:        329     253
 
SfoI GGC'GCC

Cuts at:      0     186     582 
   Size:        186     396
 
SmlI C'TyrA_G

Cuts at:      0     231     582 
   Size:        231     351
 
SspI AAT'ATT

Cuts at:      0     227     582 
   Size:        227     355
 
StyI C'CwwG_G

Cuts at:      0     267     437     582 
   Size:        267     170     145

  Fragments arranged by size: 

                 267     170     145
 
XhoI C'TCGA_G

Cuts at:      0     231     582 
   Size:        231     351
 

 Enzymes that do cut:

     AcuI    AlwNI     AvaI     BanI     BbeI     BglI     BlpI    BmgBI
     BmrI   Bpu10I     BsaI    BsaHI     BsgI     BsmI    BsrBI    BstYI
     BtsI  Eco57MI    HaeII     KasI     MmeI     NarI     PfoI     SfoI
     SmlI     SspI     StyI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII Bme1580I     BmtI
     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI