(Linear) (Six-base) MAPSORT of: KIEE5249.seq  Check: 4499  from: 1  to: 546



KIEE5249.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     462     546 
   Size:        462      84
 
AhdI GACnn_n'nnGTC

Cuts at:      0      38     546 
   Size:         38     508
 
AvaI C'yCGr_G

Cuts at:      0     109     546 
   Size:        109     437
 
BanI G'GyrC_C

Cuts at:      0      52     397     546 
   Size:         52     345     149

  Fragments arranged by size: 

                 345     149      52
 
BbeI G_GCGC'C

Cuts at:      0      56     546 
   Size:         56     490
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     213     546 
   Size:        213     333
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     197     546 
   Size:        197     349
 
Bme1580I G_kGCm'C

Cuts at:      0     400     546 
   Size:        400     146
 
BsaHI Gr'CG_yC

Cuts at:      0      53     546 
   Size:         53     493
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     512     546 
   Size:        512      34
 
BseYI C'CCAG_C

Cuts at:      0     138     200     546 
   Size:        138      62     346

  Fragments arranged by size: 

                 346     138      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     546 
   Size:        256     290
 
Bsp1286I G_dGCh'C

Cuts at:      0     400     546 
   Size:        400     146
 
BsrDI GCAATG_nn'

Cuts at:      0     516     546 
   Size:        516      30
 
BsrGI T'GTAC_A

Cuts at:      0     284     546 
   Size:        284     262
 
BssHII G'CGCG_C

Cuts at:      0     417     546 
   Size:        417     129
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     135     546 
   Size:        135     411
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     528     546 
   Size:        528      18
 
Bsu36I CC'TnA_GG

Cuts at:      0     391     546 
   Size:        391     155
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     342     546 
   Size:        342     204
 
EaeI y'GGCC_r

Cuts at:      0       2     298     515     546 
   Size:          2     296     217      31

  Fragments arranged by size: 

                 296     217      31       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     253     546 
   Size:        253     293
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     462     546 
   Size:        462      84
 
FspI TGC'GCA

Cuts at:      0      47     546 
   Size:         47     499
 
HaeII r_GCGC'y

Cuts at:      0      56      76     118     160     332     546 
   Size:         56      20      42      42     172     214

  Fragments arranged by size: 

                 214     172      56      42      42      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      24      30      56     370     402     546 
   Size:         24       6      26     314      32     144

  Fragments arranged by size: 

                 314     144      32      26      24       6
 
HincII GTy'rAC

Cuts at:      0     378     546 
   Size:        378     168
 
KasI G'GCGC_C

Cuts at:      0      52     546 
   Size:         52     494
 
MscI TGG'CCA

Cuts at:      0     300     546 
   Size:        300     246
 
MspA1I CmG'CkG

Cuts at:      0     132     270     546 
   Size:        132     138     276

  Fragments arranged by size: 

                 276     138     132
 
NarI GG'CG_CC

Cuts at:      0      53     546 
   Size:         53     493
 
PshAI GACnn'nnGTC

Cuts at:      0     241     546 
   Size:        241     305
 
PsiI TTA'TAA

Cuts at:      0     410     546 
   Size:        410     136
 
PstI C_TGCA'G

Cuts at:      0     131     546 
   Size:        131     415
 
PvuII CAG'CTG

Cuts at:      0     132     270     546 
   Size:        132     138     276

  Fragments arranged by size: 

                 276     138     132
 
SfcI C'TryA_G

Cuts at:      0     127     546 
   Size:        127     419
 
SfoI GGC'GCC

Cuts at:      0      54     546 
   Size:         54     492
 
StuI AGG'CCT

Cuts at:      0     490     546 
   Size:        490      56
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     192     546 
   Size:        192     354
 
TatI w'GTAC_w

Cuts at:      0     284     546 
   Size:        284     262
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     525     546 
   Size:        525      21
 

 Enzymes that do cut:

     AcuI     AhdI     AvaI     BanI     BbeI     BbsI    BciVI Bme1580I
    BsaHI    BseRI    BseYI     BsgI Bsp1286I    BsrDI    BsrGI   BssHII
   BstAPI    BstXI   Bsu36I    BtgZI     EaeI     EarI  Eco57MI     FspI
    HaeII    Hin4I   HincII     KasI     MscI   MspA1I     NarI    PshAI
     PsiI     PstI    PvuII     SfcI     SfoI     StuI    TaqII     TatI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BssSI    BstBI   BstEII    BstYI
  BstZ17I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI