(Linear) (Six-base) MAPSORT of: KIEE5248.seq  Check: 3023  from: 1  to: 612



KIEE5248.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     612 
   Size:        514      98
 
ApoI r'AATT_y

Cuts at:      0     181     248     314     612 
   Size:        181      67      66     298

  Fragments arranged by size: 

                 298     181      67      66
 
AseI AT'TA_AT

Cuts at:      0      80     612 
   Size:         80     532
 
BlpI GC'TnA_GC

Cuts at:      0     160     612 
   Size:        160     452
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     299     612 
   Size:         83     216     313

  Fragments arranged by size: 

                 313     216      83
 
Bpu10I CC'TnA_GC

Cuts at:      0     578     605     612 
   Size:        578      27       7

  Fragments arranged by size: 

                 578      27       7
 
BsaBI GATnn'nnATC

Cuts at:      0     218     612 
   Size:        218     394
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31     612 
   Size:         31     581
 
BsiHKAI G_wGCw'C

Cuts at:      0     166     285     612 
   Size:        166     119     327

  Fragments arranged by size: 

                 327     166     119
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      43     480     612 
   Size:         43     437     132

  Fragments arranged by size: 

                 437     132      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     166     285     612 
   Size:        166     119     327

  Fragments arranged by size: 

                 327     166     119
 
BstYI r'GATC_y

Cuts at:      0     600     612 
   Size:        600      12
 
EarI CTCTTCn'nnn_

Cuts at:      0      88     125     612 
   Size:         88      37     487

  Fragments arranged by size: 

                 487      88      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     299     514     612 
   Size:         83     216     215      98

  Fragments arranged by size: 

                 216     215      98      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0     122     612 
   Size:        122     490
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      66      98     612 
   Size:         66      32     514

  Fragments arranged by size: 

                 514      66      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     122     612 
   Size:        122     490
 
SanDI GG'GwC_CC

Cuts at:      0     122     612 
   Size:        122     490
 
SpeI A'CTAG_T

Cuts at:      0     204     612 
   Size:        204     408
 
XmnI GAAnn'nnTTC

Cuts at:      0      97     612 
   Size:         97     515
 

 Enzymes that do cut:

     AcuI     ApoI     AseI     BlpI     BpmI   Bpu10I    BsaBI    BseRI
  BsiHKAI    BsmBI Bsp1286I    BstYI     EarI  Eco57MI EcoO109I    Hin4I
    PpuMI    SanDI     SpeI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI