Restriction map for
pFN21AE5246
(Linear) (Six-base) MAPSORT of: KIEE5246.seq Check: 9221 from: 1 to: 729
KIEE5246.orf
With 182 enzymes: SgfI *
January 29, 2014 11:52 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 729
Size: 448 281
ApoI r'AATT_y
Cuts at: 0 448 729
Size: 448 281
BanII G_rGCy'C
Cuts at: 0 333 729
Size: 333 396
BciVI GTATCCnnnnn_n'
Cuts at: 0 560 729
Size: 560 169
BglI GCCn_nnn'nGGC
Cuts at: 0 48 729
Size: 48 681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 729
Size: 244 485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 493 729
Size: 109 384 236
Fragments arranged by size:
384 236 109
BsaI GGTCTCn'nnnn_
Cuts at: 0 220 729
Size: 220 509
BsaBI GATnn'nnATC
Cuts at: 0 73 287 729
Size: 73 214 442
Fragments arranged by size:
442 214 73
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 520 729
Size: 520 209
BsiHKAI G_wGCw'C
Cuts at: 0 333 645 729
Size: 333 312 84
Fragments arranged by size:
333 312 84
BsmI GAATG_Cn'
Cuts at: 0 421 729
Size: 421 308
Bsp1286I G_dGCh'C
Cuts at: 0 333 645 729
Size: 333 312 84
Fragments arranged by size:
333 312 84
BsrBI CCG'CTC
Cuts at: 0 549 729
Size: 549 180
BstXI CCAn_nnnn'nTGG
Cuts at: 0 24 729
Size: 24 705
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 477 512 729
Size: 477 35 217
Fragments arranged by size:
477 217 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 634 729
Size: 634 95
EaeI y'GGCC_r
Cuts at: 0 207 729
Size: 207 522
EarI CTCTTCn'nnn_
Cuts at: 0 458 650 729
Size: 458 192 79
Fragments arranged by size:
458 192 79
EcoICRI GAG'CTC
Cuts at: 0 331 729
Size: 331 398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 448 729
Size: 244 204 281
Fragments arranged by size:
281 244 204
EcoNI CCTnn'n_nnAGG
Cuts at: 0 442 729
Size: 442 287
EcoO109I rG'GnC_Cy
Cuts at: 0 20 110 366 627 729
Size: 20 90 256 261 102
Fragments arranged by size:
261 256 102 90 20
EcoRI G'AATT_C
Cuts at: 0 448 729
Size: 448 281
EcoRV GAT'ATC
Cuts at: 0 611 729
Size: 611 118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 487 729
Size: 487 242
HincII GTy'rAC
Cuts at: 0 156 241 729
Size: 156 85 488
Fragments arranged by size:
488 156 85
MscI TGG'CCA
Cuts at: 0 209 729
Size: 209 520
NdeI CA'TA_TG
Cuts at: 0 312 729
Size: 312 417
PasI CC'CwG_GG
Cuts at: 0 24 46 729
Size: 24 22 683
Fragments arranged by size:
683 24 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 623 729
Size: 623 106
PfoI T'CCnGG_A
Cuts at: 0 56 221 291 729
Size: 56 165 70 438
Fragments arranged by size:
438 165 70 56
PpuMI rG'GwC_Cy
Cuts at: 0 20 110 366 627 729
Size: 20 90 256 261 102
Fragments arranged by size:
261 256 102 90 20
SacI G_AGCT'C
Cuts at: 0 333 729
Size: 333 396
SanDI GG'GwC_CC
Cuts at: 0 627 729
Size: 627 102
SapI GCTCTTCn'nnn_
Cuts at: 0 650 729
Size: 650 79
SfcI C'TryA_G
Cuts at: 0 78 729
Size: 78 651
SmlI C'TyrA_G
Cuts at: 0 124 472 729
Size: 124 348 257
Fragments arranged by size:
348 257 124
StyI C'CwwG_G
Cuts at: 0 380 729
Size: 380 349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 387 729
Size: 387 342
XmnI GAAnn'nnTTC
Cuts at: 0 467 729
Size: 467 262
Enzymes that do cut:
AcuI ApoI BanII BciVI BglI BpmI BpuEI BsaI
BsaBI BseRI BsiHKAI BsmI Bsp1286I BsrBI BstXI CspCI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HincII MscI NdeI PasI PflMI PfoI
PpuMI SacI SanDI SapI SfcI SmlI StyI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII EagI EciI FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI ZraI