Restriction map for
pFN21AE5244
(Linear) (Six-base) MAPSORT of: KIEE5244.seq Check: 9927 from: 1 to: 708
KIEE5244.orf
With 182 enzymes: SgfI *
January 29, 2014 11:52 ..
AccI GT'mk_AC
Cuts at: 0 548 708
Size: 548 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 708
Size: 178 530
AflIII A'CryG_T
Cuts at: 0 650 652 708
Size: 650 2 56
Fragments arranged by size:
650 56 2
AleI CACnn'nnGTG
Cuts at: 0 127 708
Size: 127 581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 544 708
Size: 544 164
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 625 657 708
Size: 625 32 51
Fragments arranged by size:
625 51 32
AlwNI CAG_nnn'CTG
Cuts at: 0 390 393 708
Size: 390 3 315
Fragments arranged by size:
390 315 3
ApaLI G'TGCA_C
Cuts at: 0 697 708
Size: 697 11
BanI G'GyrC_C
Cuts at: 0 282 357 576 708
Size: 282 75 219 132
Fragments arranged by size:
282 219 132 75
BbeI G_GCGC'C
Cuts at: 0 286 580 708
Size: 286 294 128
Fragments arranged by size:
294 286 128
Bme1580I G_kGCm'C
Cuts at: 0 362 701 708
Size: 362 339 7
Fragments arranged by size:
362 339 7
BmgBI CAC'GTC
Cuts at: 0 153 708
Size: 153 555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 265 457 708
Size: 1 264 192 251
Fragments arranged by size:
264 251 192 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 708
Size: 85 623
BsaI GGTCTCn'nnnn_
Cuts at: 0 1 708
Size: 1 707
BsaAI yAC'GTr
Cuts at: 0 653 708
Size: 653 55
BsaHI Gr'CG_yC
Cuts at: 0 209 283 577 708
Size: 209 74 294 131
Fragments arranged by size:
294 209 131 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 52 82 319 349 625 655 708
Size: 52 30 237 30 276 30 53
Fragments arranged by size:
276 237 53 52 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 262 708
Size: 88 174 446
Fragments arranged by size:
446 174 88
BseYI C'CCAG_C
Cuts at: 0 255 274 708
Size: 255 19 434
Fragments arranged by size:
434 255 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 708
Size: 523 185
BsiHKAI G_wGCw'C
Cuts at: 0 443 701 708
Size: 443 258 7
Fragments arranged by size:
443 258 7
BsiWI C'GTAC_G
Cuts at: 0 603 708
Size: 603 105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 244 708
Size: 244 464
Bsp1286I G_dGCh'C
Cuts at: 0 362 443 701 708
Size: 362 81 258 7
Fragments arranged by size:
362 258 81 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 431 708
Size: 131 300 277
Fragments arranged by size:
300 277 131
BsrFI r'CCGG_y
Cuts at: 0 310 686 708
Size: 310 376 22
Fragments arranged by size:
376 310 22
BssHII G'CGCG_C
Cuts at: 0 396 426 428 560 708
Size: 396 30 2 132 148
Fragments arranged by size:
396 148 132 30 2
BssSI C'ACGA_G
Cuts at: 0 442 708
Size: 442 266
BstAPI GCAn_nnn'nTGC
Cuts at: 0 393 708
Size: 393 315
BstYI r'GATC_y
Cuts at: 0 671 708
Size: 671 37
BtgI C'CryG_G
Cuts at: 0 125 587 708
Size: 125 462 121
Fragments arranged by size:
462 125 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 640 708
Size: 640 68
BtsI GCAGTG_nn'
Cuts at: 0 60 708
Size: 60 648
DraIII CAC_nnn'GTG
Cuts at: 0 557 708
Size: 557 151
EaeI y'GGCC_r
Cuts at: 0 512 708
Size: 512 196
EarI CTCTTCn'nnn_
Cuts at: 0 88 708
Size: 88 620
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 559 708
Size: 559 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 178 265 457 708
Size: 1 177 87 192 251
Fragments arranged by size:
251 192 177 87 1
EcoO109I rG'GnC_Cy
Cuts at: 0 143 708
Size: 143 565
HaeII r_GCGC'y
Cuts at: 0 286 580 668 708
Size: 286 294 88 40
Fragments arranged by size:
294 286 88 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 42 74 708
Size: 42 32 634
Fragments arranged by size:
634 42 32
HincII GTy'rAC
Cuts at: 0 549 708
Size: 549 159
KasI G'GCGC_C
Cuts at: 0 282 576 708
Size: 282 294 132
Fragments arranged by size:
294 282 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 708
Size: 326 382
MslI CAynn'nnrTG
Cuts at: 0 127 708
Size: 127 581
MspA1I CmG'CkG
Cuts at: 0 21 78 183 390 465 589 708
Size: 21 57 105 207 75 124 119
Fragments arranged by size:
207 124 119 105 75 57 21
NaeI GCC'GGC
Cuts at: 0 312 708
Size: 312 396
NarI GG'CG_CC
Cuts at: 0 283 577 708
Size: 283 294 131
Fragments arranged by size:
294 283 131
NgoMIV G'CCGG_C
Cuts at: 0 310 708
Size: 310 398
PfoI T'CCnGG_A
Cuts at: 0 488 708
Size: 488 220
PmlI CAC'GTG
Cuts at: 0 653 708
Size: 653 55
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 625 657 708
Size: 625 32 51
Fragments arranged by size:
625 51 32
PpuMI rG'GwC_Cy
Cuts at: 0 143 708
Size: 143 565
PstI C_TGCA'G
Cuts at: 0 230 523 708
Size: 230 293 185
Fragments arranged by size:
293 230 185
PvuII CAG'CTG
Cuts at: 0 21 183 390 465 708
Size: 21 162 207 75 243
Fragments arranged by size:
243 207 162 75 21
SacII CC_GC'GG
Cuts at: 0 590 708
Size: 590 118
SalI G'TCGA_C
Cuts at: 0 547 708
Size: 547 161
SapI GCTCTTCn'nnn_
Cuts at: 0 88 708
Size: 88 620
SfcI C'TryA_G
Cuts at: 0 226 519 708
Size: 226 293 189
Fragments arranged by size:
293 226 189
SfoI GGC'GCC
Cuts at: 0 284 578 708
Size: 284 294 130
Fragments arranged by size:
294 284 130
SmlI C'TyrA_G
Cuts at: 0 100 708
Size: 100 608
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 52 84 319 351 708
Size: 52 32 235 32 357
Fragments arranged by size:
357 235 52 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 163 640 708
Size: 163 477 68
Fragments arranged by size:
477 163 68
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AloI AlwNI ApaLI
BanI BbeI Bme1580I BmgBI BpmI BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI
Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstYI BtgI
BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I
HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI
NarI NgoMIV PfoI PmlI PpiI PpuMI PstI PvuII
SacII SalI SapI SfcI SfoI SmlI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI Bpu10I BsaBI
BsaWI BsiEI BsmI BspEI BspHI BsrBI BsrDI BsrGI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MscI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI ZraI