(Linear) (Six-base) MAPSORT of: KIEE5243.seq  Check: 9576  from: 1  to: 612



KIEE5243.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:00  ..

AflIII A'CryG_T

Cuts at:      0      93     612 
   Size:         93     519
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     437     469     612 
   Size:        437      32     143

  Fragments arranged by size: 

                 437     143      32
 
ApoI r'AATT_y

Cuts at:      0     301     330     612 
   Size:        301      29     282

  Fragments arranged by size: 

                 301     282      29
 
BfrBI ATG'CAT

Cuts at:      0     235     612 
   Size:        235     377
 
Bme1580I G_kGCm'C

Cuts at:      0     381     612 
   Size:        381     231
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     612 
   Size:        103     509
 
Bsp1286I G_dGCh'C

Cuts at:      0     381     612 
   Size:        381     231
 
BsrDI GCAATG_nn'

Cuts at:      0     513     612 
   Size:        513      99
 
DraIII CAC_nnn'GTG

Cuts at:      0     385     612 
   Size:        385     227
 
EaeI y'GGCC_r

Cuts at:      0     114     510     612 
   Size:        114     396     102

  Fragments arranged by size: 

                 396     114     102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     612 
   Size:        103     509
 
EcoO109I rG'GnC_Cy

Cuts at:      0     588     612 
   Size:        588      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     174     206     612 
   Size:        174      32     406

  Fragments arranged by size: 

                 406     174      32
 
HincII GTy'rAC

Cuts at:      0      12     221     612 
   Size:         12     209     391

  Fragments arranged by size: 

                 391     209      12
 
MluI A'CGCG_T

Cuts at:      0      93     612 
   Size:         93     519
 
MslI CAynn'nnrTG

Cuts at:      0     240     612 
   Size:        240     372
 
NsiI A_TGCA'T

Cuts at:      0     237     612 
   Size:        237     375
 
NspI r_CATG'y

Cuts at:      0     496     612 
   Size:        496     116
 
PfoI T'CCnGG_A

Cuts at:      0      80     612 
   Size:         80     532
 
PpuMI rG'GwC_Cy

Cuts at:      0     588     612 
   Size:        588      24
 
PsiI TTA'TAA

Cuts at:      0      31     523     612 
   Size:         31     492      89

  Fragments arranged by size: 

                 492      89      31
 
SanDI GG'GwC_CC

Cuts at:      0     588     612 
   Size:        588      24
 

 Enzymes that do cut:

   AflIII     AloI     ApoI    BfrBI Bme1580I     BpmI Bsp1286I    BsrDI
   DraIII     EaeI  Eco57MI EcoO109I    Hin4I   HincII     MluI     MslI
     NsiI     NspI     PfoI    PpuMI     PsiI    SanDI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI