Restriction map for
pFN21AE5242
(Linear) (Six-base) MAPSORT of: KIEE5242.seq Check: 2506 from: 1 to: 603
KIEE5242.orf
With 182 enzymes: SgfI *
January 29, 2014 11:52 ..
AccI GT'mk_AC
Cuts at: 0 299 603
Size: 299 304
Acc65I G'GTAC_C
Cuts at: 0 110 603
Size: 110 493
AhdI GACnn_n'nnGTC
Cuts at: 0 271 603
Size: 271 332
AleI CACnn'nnGTG
Cuts at: 0 255 424 603
Size: 255 169 179
Fragments arranged by size:
255 179 169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 575 603
Size: 575 28
AlwNI CAG_nnn'CTG
Cuts at: 0 369 603
Size: 369 234
ApaLI G'TGCA_C
Cuts at: 0 448 603
Size: 448 155
BamHI G'GATC_C
Cuts at: 0 311 603
Size: 311 292
BanI G'GyrC_C
Cuts at: 0 84 110 603
Size: 84 26 493
Fragments arranged by size:
493 84 26
BclI T'GATC_A
Cuts at: 0 203 603
Size: 203 400
BglI GCCn_nnn'nGGC
Cuts at: 0 31 603
Size: 31 572
BlpI GC'TnA_GC
Cuts at: 0 289 603
Size: 289 314
Bme1580I G_kGCm'C
Cuts at: 0 452 603
Size: 452 151
BmrI ACTGGGnnnn_n'
Cuts at: 0 317 603
Size: 317 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 316 393 603
Size: 40 276 77 210
Fragments arranged by size:
276 210 77 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 603
Size: 340 263
BsaAI yAC'GTr
Cuts at: 0 387 603
Size: 387 216
BsaWI w'CCGG_w
Cuts at: 0 494 603
Size: 494 109
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 481 603
Size: 232 249 122
Fragments arranged by size:
249 232 122
BseYI C'CCAG_C
Cuts at: 0 138 369 400 603
Size: 138 231 31 203
Fragments arranged by size:
231 203 138 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 603
Size: 545 58
BsiHKAI G_wGCw'C
Cuts at: 0 44 452 603
Size: 44 408 151
Fragments arranged by size:
408 151 44
BsmBI CGTCTCn'nnnn_
Cuts at: 0 220 603
Size: 220 383
Bsp1286I G_dGCh'C
Cuts at: 0 44 452 603
Size: 44 408 151
Fragments arranged by size:
408 151 44
BsrFI r'CCGG_y
Cuts at: 0 25 603
Size: 25 578
BssHII G'CGCG_C
Cuts at: 0 143 603
Size: 143 460
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 603
Size: 426 177
BstYI r'GATC_y
Cuts at: 0 311 491 603
Size: 311 180 112
Fragments arranged by size:
311 180 112
BtgI C'CryG_G
Cuts at: 0 75 603
Size: 75 528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 468 603
Size: 468 135
EaeI y'GGCC_r
Cuts at: 0 5 519 603
Size: 5 514 84
Fragments arranged by size:
514 84 5
EarI CTCTTCn'nnn_
Cuts at: 0 49 252 256 603
Size: 49 203 4 347
Fragments arranged by size:
347 203 49 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 316 393 603
Size: 40 276 77 210
Fragments arranged by size:
276 210 77 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 334 442 603
Size: 334 108 161
Fragments arranged by size:
334 161 108
FseI GG_CCGG'CC
Cuts at: 0 29 603
Size: 29 574
HaeII r_GCGC'y
Cuts at: 0 408 603
Size: 408 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 257 289 603
Size: 257 32 314
Fragments arranged by size:
314 257 32
KpnI G_GTAC'C
Cuts at: 0 114 603
Size: 114 489
MscI TGG'CCA
Cuts at: 0 521 603
Size: 521 82
MslI CAynn'nnrTG
Cuts at: 0 255 424 603
Size: 255 169 179
Fragments arranged by size:
255 179 169
MspA1I CmG'CkG
Cuts at: 0 77 174 282 525 603
Size: 77 97 108 243 78
Fragments arranged by size:
243 108 97 78 77
NaeI GCC'GGC
Cuts at: 0 27 603
Size: 27 576
NgoMIV G'CCGG_C
Cuts at: 0 25 603
Size: 25 578
PflMI CCAn_nnn'nTGG
Cuts at: 0 369 603
Size: 369 234
PfoI T'CCnGG_A
Cuts at: 0 409 603
Size: 409 194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 603
Size: 217 32 354
Fragments arranged by size:
354 217 32
PshAI GACnn'nnGTC
Cuts at: 0 586 603
Size: 586 17
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 205 237 603
Size: 205 32 366
Fragments arranged by size:
366 205 32
PvuII CAG'CTG
Cuts at: 0 174 282 525 603
Size: 174 108 243 78
Fragments arranged by size:
243 174 108 78
SacII CC_GC'GG
Cuts at: 0 78 603
Size: 78 525
SapI GCTCTTCn'nnn_
Cuts at: 0 49 603
Size: 49 554
SmlI C'TyrA_G
Cuts at: 0 355 603
Size: 355 248
StuI AGG'CCT
Cuts at: 0 516 603
Size: 516 87
StyI C'CwwG_G
Cuts at: 0 320 603
Size: 320 283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 135 514 603
Size: 15 120 379 89
Fragments arranged by size:
379 120 89 15
Enzymes that do cut:
AccI Acc65I AhdI AleI AlfI AlwNI ApaLI BamHI
BanI BclI BglI BlpI Bme1580I BmrI BpmI BpuEI
BsaAI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BsrFI BssHII BstXI BstYI BtgI BtgZI EaeI EarI
Eco57MI EcoNI FseI HaeII Hin4I KpnI MscI MslI
MspA1I NaeI NgoMIV PflMI PfoI PpiI PshAI PsrI
PvuII SacII SapI SmlI StuI StyI XcmI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI
BglII BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI
BsaXI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoO109I EcoRI EcoRV FalI FspI FspAI HincII
HindIII HpaI KasI MfeI MluI MmeI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpuMI PsiI PspOMI PspXI PstI
PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI