(Linear) (Six-base) MAPSORT of: KIEE5241.seq  Check: 4983  from: 1  to: 750



KIEE5241.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:00  ..

AfeI AGC'GCT

Cuts at:      0      76     750 
   Size:         76     674
 
ApoI r'AATT_y

Cuts at:      0     742     750 
   Size:        742       8
 
AvaI C'yCGr_G

Cuts at:      0     639     750 
   Size:        639     111
 
BclI T'GATC_A

Cuts at:      0     578     750 
   Size:        578     172
 
BglII A'GATC_T

Cuts at:      0     207     750 
   Size:        207     543
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     750 
   Size:        331     419
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     553     750 
   Size:        553     197
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     369     750 
   Size:        369     381
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     304     334     750 
   Size:        304      30     416

  Fragments arranged by size: 

                 416     304      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     510     750 
   Size:        510     240
 
Bsp1286I G_dGCh'C

Cuts at:      0     510     750 
   Size:        510     240
 
BsrBI CCG'CTC

Cuts at:      0     122     750 
   Size:        122     628
 
BsrDI GCAATG_nn'

Cuts at:      0     329     750 
   Size:        329     421
 
BstBI TT'CG_AA

Cuts at:      0      80     750 
   Size:         80     670
 
BstYI r'GATC_y

Cuts at:      0      26     207     750 
   Size:         26     181     543

  Fragments arranged by size: 

                 543     181      26
 
BtgI C'CryG_G

Cuts at:      0      47     750 
   Size:         47     703
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     323     358     750 
   Size:        323      35     392

  Fragments arranged by size: 

                 392     323      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     165     750 
   Size:        165     585
 
EaeI y'GGCC_r

Cuts at:      0      44     606     750 
   Size:         44     562     144

  Fragments arranged by size: 

                 562     144      44
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     132     750 
   Size:         19     113     618

  Fragments arranged by size: 

                 618     113      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     750 
   Size:        331     419
 
HaeII r_GCGC'y

Cuts at:      0      78     750 
   Size:         78     672
 
MscI TGG'CCA

Cuts at:      0     608     750 
   Size:        608     142
 
PstI C_TGCA'G

Cuts at:      0     155     750 
   Size:        155     595
 
ScaI AGT'ACT

Cuts at:      0     707     750 
   Size:        707      43
 
SfcI C'TryA_G

Cuts at:      0     151     750 
   Size:        151     599
 
SmaI CCC'GGG

Cuts at:      0     641     750 
   Size:        641     109
 
SmlI C'TyrA_G

Cuts at:      0     568     750 
   Size:        568     182
 
TatI w'GTAC_w

Cuts at:      0     445     705     750 
   Size:        445     260      45

  Fragments arranged by size: 

                 445     260      45
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      48      80     750 
   Size:         48      32     670

  Fragments arranged by size: 

                 670      48      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      45     750 
   Size:         45     705
 
XmaI C'CCGG_G

Cuts at:      0     639     750 
   Size:        639     111
 
XmnI GAAnn'nnTTC

Cuts at:      0      76     750 
   Size:         76     674
 

 Enzymes that do cut:

     AfeI     ApoI     AvaI     BclI    BglII     BpmI    BpuEI     BsaI
    BsaXI  BsiHKAI Bsp1286I    BsrBI    BsrDI    BstBI    BstYI     BtgI
    CspCI   DraIII     EaeI     EciI  Eco57MI    HaeII     MscI     PstI
     ScaI     SfcI     SmaI     SmlI     TatI     TstI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
     DraI     DrdI     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I
     XbaI     XhoI     ZraI