(Linear) (Six-base) MAPSORT of: KIEE5240.seq  Check: 6737  from: 1  to: 753



KIEE5240.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     690     753 
   Size:        690      63
 
AvaI C'yCGr_G

Cuts at:      0     581     600     753 
   Size:        581      19     153

  Fragments arranged by size: 

                 581     153      19
 
BamHI G'GATC_C

Cuts at:      0     419     753 
   Size:        419     334
 
BanI G'GyrC_C

Cuts at:      0      76     753 
   Size:         76     677
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      92     753 
   Size:         92     661
 
BclI T'GATC_A

Cuts at:      0     329     753 
   Size:        329     424
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     603     696     753 
   Size:        603      93      57

  Fragments arranged by size: 

                 603      93      57
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     178     753 
   Size:        178     575
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     753 
   Size:        129     624
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     611     753 
   Size:        611     142
 
BsaBI GATnn'nnATC

Cuts at:      0      31     576     753 
   Size:         31     545     177

  Fragments arranged by size: 

                 545     177      31
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     154     331     753 
   Size:        154     177     422

  Fragments arranged by size: 

                 422     177     154
 
BsiEI CG_ry'CG

Cuts at:      0      99     753 
   Size:         99     654
 
BsrDI GCAATG_nn'

Cuts at:      0     122     753 
   Size:        122     631
 
BsrFI r'CCGG_y

Cuts at:      0     491     753 
   Size:        491     262
 
BstBI TT'CG_AA

Cuts at:      0     501     753 
   Size:        501     252
 
BstYI r'GATC_y

Cuts at:      0     156     419     753 
   Size:        156     263     334

  Fragments arranged by size: 

                 334     263     156
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      18     753 
   Size:         18     735
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     103     753 
   Size:        103     650
 
EaeI y'GGCC_r

Cuts at:      0     179     753 
   Size:        179     574
 
EarI CTCTTCn'nnn_

Cuts at:      0     634     753 
   Size:        634     119
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     690     753 
   Size:        129     561      63

  Fragments arranged by size: 

                 561     129      63
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     240     753 
   Size:        240     513
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     597     629     753 
   Size:        597      32     124

  Fragments arranged by size: 

                 597     124      32
 
HindIII A'AGCT_T

Cuts at:      0     335     753 
   Size:        335     418
 
MscI TGG'CCA

Cuts at:      0     181     753 
   Size:        181     572
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     671     753 
   Size:        671      82
 
PspXI vC'TCGA_Gb

Cuts at:      0     600     753 
   Size:        600     153
 
SfcI C'TryA_G

Cuts at:      0     540     753 
   Size:        540     213
 
SmlI C'TyrA_G

Cuts at:      0     600     753 
   Size:        600     153
 
StyI C'CwwG_G

Cuts at:      0      38     200     753 
   Size:         38     162     553

  Fragments arranged by size: 

                 553     162      38
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     116     148     753 
   Size:        116      32     605

  Fragments arranged by size: 

                 605     116      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     271     753 
   Size:        271     482
 
XhoI C'TCGA_G

Cuts at:      0     600     753 
   Size:        600     153
 

 Enzymes that do cut:

     AcuI     AvaI    BamHI     BanI     BbsI     BclI     BmrI     BplI
     BpmI     BsaI    BsaBI    BseRI    BsiEI    BsrDI    BsrFI    BstBI
    BstYI    BtgZI     DrdI     EaeI     EarI  Eco57MI     FalI    Hin4I
  HindIII     MscI    PflMI    PspXI     SfcI     SmlI     StyI     TstI
  Tth111I     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     XbaI     XcmI     XmaI
     XmnI     ZraI