(Linear) (Six-base) MAPSORT of: KIEE5236.seq  Check: 4777  from: 1  to: 753



KIEE5236.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     753 
   Size:        251     502
 
AflIII A'CryG_T

Cuts at:      0     350     747     753 
   Size:        350     397       6

  Fragments arranged by size: 

                 397     350       6
 
ApoI r'AATT_y

Cuts at:      0     201     502     753 
   Size:        201     301     251

  Fragments arranged by size: 

                 301     251     201
 
BanI G'GyrC_C

Cuts at:      0      42     165     753 
   Size:         42     123     588

  Fragments arranged by size: 

                 588     123      42
 
BanII G_rGCy'C

Cuts at:      0     694     753 
   Size:        694      59
 
Bme1580I G_kGCm'C

Cuts at:      0      47      66     168     753 
   Size:         47      19     102     585

  Fragments arranged by size: 

                 585     102      47      19
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      97     732     753 
   Size:         97     635      21

  Fragments arranged by size: 

                 635      97      21
 
BsaAI yAC'GTr

Cuts at:      0     450     753 
   Size:        450     303
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     753 
   Size:         58     695
 
BseYI C'CCAG_C

Cuts at:      0     669     753 
   Size:        669      84
 
Bsp1286I G_dGCh'C

Cuts at:      0      47      66     168     694     753 
   Size:         47      19     102     526      59

  Fragments arranged by size: 

                 526     102      59      47      19
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     296     753 
   Size:        296     457
 
BsrBI CCG'CTC

Cuts at:      0      33     332     753 
   Size:         33     299     421

  Fragments arranged by size: 

                 421     299      33
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     669     753 
   Size:        669      84
 
BtgI C'CryG_G

Cuts at:      0     573     753 
   Size:        573     180
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     397     753 
   Size:        397     356
 
BtsI GCAGTG_nn'

Cuts at:      0      94     656     753 
   Size:         94     562      97

  Fragments arranged by size: 

                 562      97      94
 
DraIII CAC_nnn'GTG

Cuts at:      0     450     753 
   Size:        450     303
 
EaeI y'GGCC_r

Cuts at:      0     468     753 
   Size:        468     285
 
EarI CTCTTCn'nnn_

Cuts at:      0     183     753 
   Size:        183     570
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     753 
   Size:        251     502
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     529     753 
   Size:        529     224
 
EcoO109I rG'GnC_Cy

Cuts at:      0     220     753 
   Size:        220     533
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     541     567     753 
   Size:        541      26     186

  Fragments arranged by size: 

                 541     186      26
 
HindIII A'AGCT_T

Cuts at:      0     145     753 
   Size:        145     608
 
MspA1I CmG'CkG

Cuts at:      0     575     753 
   Size:        575     178
 
NdeI CA'TA_TG

Cuts at:      0     361     696     753 
   Size:        361     335      57

  Fragments arranged by size: 

                 361     335      57
 
NspI r_CATG'y

Cuts at:      0     354     669     741     753 
   Size:        354     315      72      12

  Fragments arranged by size: 

                 354     315      72      12
 
PciI A'CATG_T

Cuts at:      0     350     753 
   Size:        350     403
 
PpuMI rG'GwC_Cy

Cuts at:      0     220     753 
   Size:        220     533
 
SacII CC_GC'GG

Cuts at:      0     576     753 
   Size:        576     177
 
ScaI AGT'ACT

Cuts at:      0     686     753 
   Size:        686      67
 
SexAI A'CCwGG_T

Cuts at:      0     530     753 
   Size:        530     223
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     265     478     753 
   Size:        265     213     275

  Fragments arranged by size: 

                 275     265     213
 
TatI w'GTAC_w

Cuts at:      0     684     753 
   Size:        684      69
 
XbaI T'CTAG_A

Cuts at:      0     582     753 
   Size:        582     171
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     BanI    BanII Bme1580I     BmrI    BsaAI
    BseRI    BseYI Bsp1286I    BspMI    BsrBI    BstXI     BtgI    BtgZI
     BtsI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FalI
  HindIII   MspA1I     NdeI     NspI     PciI    PpuMI    SacII     ScaI
    SexAI    TaqII     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI