(Linear) (Six-base) MAPSORT of: KIEE5233.seq  Check: 1653  from: 1  to: 594



KIEE5233.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AccI GT'mk_AC

Cuts at:      0     159     594 
   Size:        159     435
 
ApoI r'AATT_y

Cuts at:      0     177     190     213     356     451     594 
   Size:        177      13      23     143      95     143

  Fragments arranged by size: 

                 177     143     143      95      23      13
 
BanI G'GyrC_C

Cuts at:      0     463     594 
   Size:        463     131
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     331     594 
   Size:        331     263
 
BglII A'GATC_T

Cuts at:      0     573     594 
   Size:        573      21
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      38     594 
   Size:         38     556
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     286     594 
   Size:        286     308
 
BstYI r'GATC_y

Cuts at:      0     573     594 
   Size:        573      21
 
EaeI y'GGCC_r

Cuts at:      0     544     594 
   Size:        544      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     263     283     594 
   Size:        263      20     311

  Fragments arranged by size: 

                 311     263      20
 
MscI TGG'CCA

Cuts at:      0     546     594 
   Size:        546      48
 
MspA1I CmG'CkG

Cuts at:      0      26     594 
   Size:         26     568
 
ScaI AGT'ACT

Cuts at:      0     127     594 
   Size:        127     467
 
SfcI C'TryA_G

Cuts at:      0     206     347     594 
   Size:        206     141     247

  Fragments arranged by size: 

                 247     206     141
 
StyI C'CwwG_G

Cuts at:      0      49     594 
   Size:         49     545
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     234     594 
   Size:        234     360
 
TatI w'GTAC_w

Cuts at:      0     125     594 
   Size:        125     469
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      74     106     594 
   Size:         74      32     488

  Fragments arranged by size: 

                 488      74      32
 

 Enzymes that do cut:

     AccI     ApoI     BanI     BbsI    BglII     BsaI    BseRI    BstYI
     EaeI     EarI     MscI   MspA1I     ScaI     SfcI     StyI    TaqII
     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI