Restriction map for
pFN21AE5229
(Linear) (Six-base) MAPSORT of: KIEE5229.seq Check: 4215 from: 1 to: 558
KIEE5229.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
AfeI AGC'GCT
Cuts at: 0 361 558
Size: 361 197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 337 369 558
Size: 337 32 189
Fragments arranged by size:
337 189 32
AvaI C'yCGr_G
Cuts at: 0 56 558
Size: 56 502
BamHI G'GATC_C
Cuts at: 0 227 558
Size: 227 331
BanI G'GyrC_C
Cuts at: 0 145 558
Size: 145 413
BanII G_rGCy'C
Cuts at: 0 398 558
Size: 398 160
BbeI G_GCGC'C
Cuts at: 0 149 558
Size: 149 409
BbvCI CC'TCA_GC
Cuts at: 0 170 558
Size: 170 388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 319 353 558
Size: 319 34 205
Fragments arranged by size:
319 205 34
BmgBI CAC'GTC
Cuts at: 0 314 558
Size: 314 244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 558
Size: 364 194
Bpu10I CC'TnA_GC
Cuts at: 0 170 558
Size: 170 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 558
Size: 115 443
BsaI GGTCTCn'nnnn_
Cuts at: 0 66 415 558
Size: 66 349 143
Fragments arranged by size:
349 143 66
BsaAI yAC'GTr
Cuts at: 0 372 558
Size: 372 186
BsaHI Gr'CG_yC
Cuts at: 0 44 146 558
Size: 44 102 412
Fragments arranged by size:
412 102 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 558
Size: 337 30 191
Fragments arranged by size:
337 191 30
BseYI C'CCAG_C
Cuts at: 0 117 558
Size: 117 441
BsiHKAI G_wGCw'C
Cuts at: 0 398 558
Size: 398 160
Bsp1286I G_dGCh'C
Cuts at: 0 398 558
Size: 398 160
BsrFI r'CCGG_y
Cuts at: 0 293 558
Size: 293 265
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 558
Size: 282 276
BstYI r'GATC_y
Cuts at: 0 227 558
Size: 227 331
BtgI C'CryG_G
Cuts at: 0 323 558
Size: 323 235
DrdI GACnn_nn'nnGTC
Cuts at: 0 312 558
Size: 312 246
EcoICRI GAG'CTC
Cuts at: 0 396 558
Size: 396 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 558
Size: 364 194
EcoNI CCTnn'n_nnAGG
Cuts at: 0 274 558
Size: 274 284
EcoO109I rG'GnC_Cy
Cuts at: 0 279 558
Size: 279 279
HaeII r_GCGC'y
Cuts at: 0 149 167 363 558
Size: 149 18 196 195
Fragments arranged by size:
196 195 149 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 251 283 558
Size: 251 32 275
Fragments arranged by size:
275 251 32
KasI G'GCGC_C
Cuts at: 0 145 558
Size: 145 413
MspA1I CmG'CkG
Cuts at: 0 205 325 558
Size: 205 120 233
Fragments arranged by size:
233 205 120
NarI GG'CG_CC
Cuts at: 0 146 558
Size: 146 412
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 558
Size: 372 186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 558
Size: 337 32 189
Fragments arranged by size:
337 189 32
SacI G_AGCT'C
Cuts at: 0 398 558
Size: 398 160
SacII CC_GC'GG
Cuts at: 0 326 558
Size: 326 232
SfoI GGC'GCC
Cuts at: 0 147 558
Size: 147 411
SmaI CCC'GGG
Cuts at: 0 58 558
Size: 58 500
SmlI C'TyrA_G
Cuts at: 0 130 558
Size: 130 428
StyI C'CwwG_G
Cuts at: 0 245 275 558
Size: 245 30 283
Fragments arranged by size:
283 245 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 406 558
Size: 406 152
XmaI C'CCGG_G
Cuts at: 0 56 558
Size: 56 502
Enzymes that do cut:
AfeI AloI AvaI BamHI BanI BanII BbeI BbvCI
BcgI BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseYI BsiHKAI Bsp1286I BsrFI BstXI BstYI BtgI
DrdI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MspA1I NarI PflMI PpiI SacI SacII SfoI SmaI
SmlI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BsaBI BsaWI BseRI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EaeI EagI EarI EciI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI