Restriction map for
pFN21AE5228
(Linear) (Six-base) MAPSORT of: KIEE5228.seq Check: 6693 from: 1 to: 708
KIEE5228.orf
With 182 enzymes: SgfI *
April 26, 2013 16:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 295 708
Size: 295 413
AleI CACnn'nnGTG
Cuts at: 0 502 708
Size: 502 206
AlwNI CAG_nnn'CTG
Cuts at: 0 267 708
Size: 267 441
AvaI C'yCGr_G
Cuts at: 0 528 708
Size: 528 180
BbsI GAAGACnn'nnnn_
Cuts at: 0 39 51 448 708
Size: 39 12 397 260
Fragments arranged by size:
397 260 39 12
BclI T'GATC_A
Cuts at: 0 641 708
Size: 641 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 255 708
Size: 255 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 160 459 708
Size: 92 68 299 249
Fragments arranged by size:
299 249 92 68
Bpu10I CC'TnA_GC
Cuts at: 0 664 708
Size: 664 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 708
Size: 493 215
BsaBI GATnn'nnATC
Cuts at: 0 646 708
Size: 646 62
BsaHI Gr'CG_yC
Cuts at: 0 416 708
Size: 416 292
BsaWI w'CCGG_w
Cuts at: 0 689 708
Size: 689 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 577 607 708
Size: 577 30 101
Fragments arranged by size:
577 101 30
BseYI C'CCAG_C
Cuts at: 0 144 270 708
Size: 144 126 438
Fragments arranged by size:
438 144 126
BsiHKAI G_wGCw'C
Cuts at: 0 509 708
Size: 509 199
BsmBI CGTCTCn'nnnn_
Cuts at: 0 448 708
Size: 448 260
Bsp1286I G_dGCh'C
Cuts at: 0 509 708
Size: 509 199
BspEI T'CCGG_A
Cuts at: 0 689 708
Size: 689 19
BspHI T'CATG_A
Cuts at: 0 212 644 708
Size: 212 432 64
Fragments arranged by size:
432 212 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 44 295 708
Size: 44 251 413
Fragments arranged by size:
413 251 44
BsrDI GCAATG_nn'
Cuts at: 0 617 708
Size: 617 91
BsrFI r'CCGG_y
Cuts at: 0 322 708
Size: 322 386
BssSI C'ACGA_G
Cuts at: 0 451 708
Size: 451 257
BstAPI GCAn_nnn'nTGC
Cuts at: 0 23 708
Size: 23 685
BstXI CCAn_nnnn'nTGG
Cuts at: 0 435 708
Size: 435 273
BtgI C'CryG_G
Cuts at: 0 401 708
Size: 401 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 180 708
Size: 180 528
ClaI AT'CG_AT
Cuts at: 0 581 708
Size: 581 127
DraIII CAC_nnn'GTG
Cuts at: 0 23 708
Size: 23 685
EaeI y'GGCC_r
Cuts at: 0 317 708
Size: 317 391
EarI CTCTTCn'nnn_
Cuts at: 0 46 559 595 708
Size: 46 513 36 113
Fragments arranged by size:
513 113 46 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 708
Size: 94 614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 160 459 708
Size: 92 68 299 249
Fragments arranged by size:
299 249 92 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 394 556 708
Size: 394 162 152
Fragments arranged by size:
394 162 152
EcoO109I rG'GnC_Cy
Cuts at: 0 387 708
Size: 387 321
HaeII r_GCGC'y
Cuts at: 0 209 708
Size: 209 499
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 65 97 577 609 708
Size: 65 32 480 32 99
Fragments arranged by size:
480 99 65 32 32
HincII GTy'rAC
Cuts at: 0 342 708
Size: 342 366
MslI CAynn'nnrTG
Cuts at: 0 199 502 708
Size: 199 303 206
Fragments arranged by size:
303 206 199
NaeI GCC'GGC
Cuts at: 0 324 708
Size: 324 384
NgoMIV G'CCGG_C
Cuts at: 0 322 708
Size: 322 386
PfoI T'CCnGG_A
Cuts at: 0 251 595 708
Size: 251 344 113
Fragments arranged by size:
344 251 113
SapI GCTCTTCn'nnn_
Cuts at: 0 46 708
Size: 46 662
SfcI C'TryA_G
Cuts at: 0 391 708
Size: 391 317
SmlI C'TyrA_G
Cuts at: 0 508 708
Size: 508 200
StyI C'CwwG_G
Cuts at: 0 359 428 708
Size: 359 69 280
Fragments arranged by size:
359 280 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 708
Size: 76 632
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 247 279 708
Size: 247 32 429
Fragments arranged by size:
429 247 32
XmnI GAAnn'nnTTC
Cuts at: 0 373 445 708
Size: 373 72 263
Fragments arranged by size:
373 263 72
Enzymes that do cut:
AarI AleI AlwNI AvaI BbsI BclI BmrI BpmI
Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseYI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BssSI
BstAPI BstXI BtgI BtgZI ClaI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MslI
NaeI NgoMIV PfoI SapI SfcI SmlI StyI TaqII
TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI BsaI BsaAI BseRI BsgI BsiEI
BsiWI BsmI BsrBI BsrGI BssHII BstBI BstEII BstYI
BstZ17I Bsu36I BtsI CspCI DraI DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI ZraI